Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... sequence Primer pair Annealing temp ( C) C5 3 (sense) GTGCTTGC GAATTCCCCGGGA C5 3 C2 03 60 C2 03 (antisense) CTC GAATTCAGTTGACGCCGTCTTCCAGAACC N294 (sense) CGTAGACCGTGCACCAGCACGAATCCTAAAC N294–N295 66 N295 ... (antisense) G GAATTCCAGCGGTTTAAACTCAATG N222 (antisense) CTC GAATTCAGTTCACGCCGTCTTCCAG N298–N222 64 C5 4 (antisense) CTC GAATTCCACTAGGTAGGCCGAAG C5 3 C5 4 60 Expression of the...
Ngày tải lên: 30/03/2014, 03:20
Báo cáo khoa học: Metabolic pathway that produces essential fatty acids from polymethylene-interrupted polyunsaturated fatty acids in animal cells pot
... acid-supplemented cells. In neither the control cells nor the cells cultured with dihomo -c- linolenic acid was it present at a detectable level. The formation of 16:2 D-7,10 and accumulation of linoleic acid in ... and ZP102 cells were incubated with sciadonic acid, sciadonic acid was incorporated into the cellular lipids of both (Fig. 5A ,C) . Sciadonic acid- depend...
Ngày tải lên: 23/03/2014, 09:20
Báo cáo khoa học: NMR and MS evidences for a random assembled O-specific chain structure in the LPS of the bacterium Xanthomonas campestris pv. Vitians A case of unsystematic biosynthetic polymerization potx
... reaction can be conducted according to the ABC transporter pathway in which subsequent residues are added by the glycosyl transferases to the nonreducing end of the acceptor chain at the cytoplasmic ... reflected in the chemical shifts of C1 of Fuc3NAc, which is downfield in comparison to the other fragments. A change in the / and w angles has been shown to give r...
Ngày tải lên: 31/03/2014, 09:20
Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf
... reverse, 5¢-GGGGGATCCCAAGTACTGTT-3¢) and GAPDH (for- ward, 5¢-CCCATCACCATCTTCCAGGA-3¢; reverse, 5¢- TGCTTCACCACCTTCTTGAT-3¢)at94 C for 30 s, 56 C for 15 s and 72 C for 2 min, for 30 cycles. The expected ... synergistic effect on the induction of cytokines ⁄ chemokines To determine whether there is any combined effect of AGE-HSA and LPS on the induction of cytokines ⁄ chemokines...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
... ATATAAGCTTATCCTCTGATAGC AK LF GACCCATCATCGAGGACTA AK SF ACCACAAGGGTTTCAAGCAG AK LR CCACACCAGGAAGGTCTTGT AK SR GGTGGAGGAAACCTTGGACT eIF4A LF ACGTCAACATGTCCGACAAA eIF4A SF CGGTGGAGACAACAAGGACT eIF4A LR TGCGTTTCGTTTGACTTCAC eIF4A ... TGCTACAGCAACTGGTGATCAGAAGGG LR1 CCCTTCCTGATCACCATGTTGCTGT LR2 GGCCATCATACAGGTGACTAGGAGGGT LR3 GGGTGATTTGACACACGGTTTTGATGGA PWF1 ATGGGATATGTTCTCAGT CHH-SF ACAGATTTAC...
Ngày tải lên: 16/03/2014, 06:20
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... E52D-rev:5¢-GCGCCACTTCTCGACGGG GTCGGTCAGCAGGC-3¢. E52H-fwd:5¢-GCCTGCTGAC C CACCCCGTCGAGAAGTGGCGC-3¢. E52H-rev:5¢-GC GCCACTTCTCGACGGG GTGGGTCAGCAGGC-3¢. E52Q- fwd:5¢-GCCTGCTGACC CAGCCCGTCGAGAAGTGG CGC-3¢. ... E52Q-rev:5¢-GCGCCACTTCTCGACGGGCT G GGTCAGCAGGC-3¢. Y70W-fwd:5¢-CTGCTGGAGCT GATGT GGAAAGATCCCAAGAAG-3¢. Y70W-rev :5¢-CTT CTTGGGATCTTT CCACATCAGCTCCAGCAG-3¢. Q81N- fwd:5¢-TGGGCCATGCCCTTT...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc
... [38,39]. Splicing-speci c PCR was performed using the following primers: ‘a’, 5¢-TCTCCCAAAGCTTCTCGATGC-3¢ (for- ward primer speci c for the Noxo1 cDNA); ‘b’, 5¢-CCCAAAGCTTCTCGGTCAGGC-3¢ (forward ... 3663–3677 ª 2006 The Authors Journal compilation ª 2006 FEBS GGCAGGCCCCCGATACCCAG-3¢ and 5¢-CGTCTCGA G GAGGCGGCCCGCAGCGCGAGA-3¢; sequences from the mRNA are underlined. With the two pr...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... RTnI 96)181 bound actin and TnC in the absence and presence, respectively, of Ca 2+ . These phenomena should directly reflect the mechanism of Ca 2+ switching involving the alternative binding of the C- terminal ... mollusks. In molluskan troponin, the activation is probably induced by streng- thening of the interaction between the structural TnC- binding site and t...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... underlined, with the half site also present in the operator in bold). Name Length (bp) Sequence 1IR 39 GAACAAGGACAGGGCATTGACTTGTCCCTGTCCCTTAAT 1DR 45 ATACCC GGGTTTAAAGGGGACAGATTCAGGCTGTTATCCACACCC 1DR-short ... domain, which is involved in protein protein interactions in the dimeric RepA–operon complex, but which actually binds DNA in the monomeric RepA–iteron complex. Her...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... La Jolla, CA, USA), was used for the mutation of Arg213 fi Ala. The oligonucleotides u sed were: 5¢-gcgccaatcctggacgctgacgtcatcgacgcg-3¢ (forward) and 5¢-cgcgtcgatgacgtcagcgtccaggattggcgc-3¢ (reverse). ... 5¢-and3¢-ends, the respective attB1 and attB2 recombination sites were: 5¢-taacagagccg accgtcgcccgg-3¢ (forward primer) and 5¢-cttatgcgtgcaa cgccctctt-3¢ (reverse primer). The PCR...
Ngày tải lên: 07/03/2014, 16:20