Báo cáo khoa học: Structural stability of the cofactor bindi

Báo cáo khoa học: Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase – the role of evolutionarily conserved hydrophobic contacts potx

... 731 9–7 328 ª 2009 The Authors Journal compilation ª 2009 FEBS Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase – the role of evolutionarily conserved ... interactions between the subunits and are involved in cofactor binding, substrate binding and catalysis (Fig. 1). On the other hand,...

Ngày tải lên : 30/03/2014, 02:20

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Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx

... such as Escherichia coli. The only committed step is the conversion of phenylacetate into phenylacetyl-CoA. The paa operon of E. coli encodes 14 polypeptides involved in the catabolism of phenylacetate. We ... phosphate, pH 4, containing 8% (v/v) acetonitrile for 15 min and then developed with a linear gradient of 8–4 0% acetonitrile in the same buffer for 5min .T...

Ngày tải lên : 23/03/2014, 18:20

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Báo cáo khoa học: Structural origins for selectivity and speciﬁcity in an engineered bacterial repressor–inducer pair pdf

... comprises the dimer interface. The two effector- binding sites present in the dimer are identical and, because the binding sites are located within the protein interface, each binding site is lined ... shown in grey and blue). The stereo represen- tation shows that the binding position of the ligands and the orientations of the side chains lining the b...

Ngày tải lên : 16/03/2014, 00:20

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Tài liệu Báo cáo khoa học: Disulﬁde bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt

... T 2 values in the apo protein and in all of the investigated holo proteins, thus plays a key role in regulating the positioning of the helix–loop–helix motif with respect to the b-barrel in order ... whereas the bound GCA mostly in uenced the C-terminal region of the protein, in agreement with the site selectivity observed for the two ligands. Interest...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... solutions. The ligand bound was interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain car- bonyl. Binding of a xanthine base from XMP in the same position, would lead to the ... N7 (Fig. 1C). The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the ribose ar...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... regulatory inserts: an autoinhibitory insert in the FMN domain [2 7–3 0], a C-terminal tail (CT) [3 1– 33] and possibly a small insertion or b-finger in the connecting domain [34,35] (Fig. 3A,B). CaM binding to ... H 4 B cofactor rather than by the flavoprotein domain [16]. The H 4 B radical is then reduced within the enzyme by the flavoprotein domain in order to conti...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... [27]. Extending these studies, we examine here the effect of increasing the bulk of the residue at position 74. Surprisingly, the Y74W mutant exhibited loss of both activity and stability. There was ... which lies at the dimer interface of PfTIM, appears to be important in promoting subunit dissocia- tion [27] and also in maintaining the geometry of the active si...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... of binding of E-64 with ervatamin-A and ervatamin-C The modes of binding of E-64 with ervatamin-A and ervatamin-C have been analyzed and compared with the structures of other complexes of the ... sequence. As the active site for this class of enzymes is at the interface of the two domains, interdomain plasticity plays a role in the activity of the e...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... immunoglo- bulin-like domains while the intracellular part is a tyrosine kinase domain. The ligand -binding sites of the receptors are located to the three ﬁrst immuno- globulin-like domains (reviewed in ... heparin- binding domain in the C-terminal region of the growth factor domain but the exact residues responsible for the binding have not been identiﬁed [25]....

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... a C-terminal extension to the protein kinase folds back on the kinase domain and interferes with the substrate binding sites. In the case of the titin and twitchin kinases, the autoinhibitory sequence ... hexadecameric enzyme consisting of four copies of four subunits: (abcd) 4 . An intrinsic calmodulin (CaM, the d subunit) binds directly to the c protein kinase chai...

Ngày tải lên : 19/02/2014, 17:20

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