Báo cáo khoa học: Structural and mutational analyses of protein–protein interactions between transthyretin and retinol-binding protein doc

... Davis 3 and Edward Darzynkiewicz 1 1 Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Poland 2 Department of Chemistry, University of Warsaw, Poland 3 ... ª 2010 FEBS of the HIT family of pyrophosphatases. EMBO J 21, 4699–4708. 18 Brenner C (2002) Hint, Fhit, and GalT: function, struc- ture, evolution, and mechanism of...

Ngày tải lên: 29/03/2014, 09:20

... nuclear protein (Anp32a ⁄ LANP) belongs to a family of evolutionarily-conserved phosphoproteins involved in a com- plex network of protein protein interactions. In an effort to understand the cellular ... regions of the molecule. As part of a long-term function-oriented structural effort aimed at understanding the molecular bases of polyQ disease proteins, we report here a...

Ngày tải lên: 18/02/2014, 17:20

... in Chang cells On the basis of the different usages of exon 1 and exon 1a in Huh7 and Chang cells, we decided to ana- lyze the relationship between usage of exon 1a and P1 promoter methylation. ... regions of exon 8 and exon 9 in the genomic DNA of Chang cells were sequenced and aligned with Occ WT . (F) Schematic of mRNA coding sequence for human occludin, showing the...

Ngày tải lên: 18/02/2014, 18:20

... differences between the active sites of this enzyme and arginase I. In spite of the rel- atively low agmatinase activity of the Asn149Asp vari- ant, it is clear that the interactions of arginase ... properties of the wild-type, His120Asn, His145Asn and Asn149Asp variants of arginase II Purified wild-type, His120Asn and His145Asn variants of arginase II were active even...

Ngày tải lên: 20/02/2014, 02:21

... for interactions with RNA ligands, including the N7 atom of purines. Therefore, there is the possible involvement of the N7 atom of the neigh- boring tandem guanines, G27 and G28 or G37 and G38 ... ribozyme in the pres- ence of Mg 2+ demonstrated the importance of the N7 group of purines and Mg 2+ ions in the organization of the catalytic site of the ribozyme and in...

Ngày tải lên: 16/03/2014, 06:20

... availability of a range of structures of NNRTI- resistant RTs drugs will provide greater understanding of the molecular basis of drug resistance mechanisms. Such data will contribute to the design of novel ... NNRTI binding sites of wild-type and mutant RTs for the following complexes: (A) Lys101Glu and nevirapine, (B) Glu138Lys and nevirapine, and (C) Glu138Lys and PE...

Ngày tải lên: 16/03/2014, 13:20

... tation of the syntactic structure is preliminary to the understanding process (2). While the work of this second group of resear- chers was concerned mainly with the understanding of individual ... individual sentences, the work of the partisans of semantics was about the understanding of whole texts. This shifting of attention substained the idea that syntax and sema...

Ngày tải lên: 18/03/2014, 02:20

... noncytosolic location. Such analyses are known to suffer frequently from artifacts induced by the truncation of the protein studied and the nature of the reporter gene used, and therefore these studies ... the N-terminus and the long loop between HRVII and HRVIII located in the extra- cytosolic space, and consequently the C-terminus in the cytosol. The localization of th...

Ngày tải lên: 23/03/2014, 13:20

... fragments ‘book and newspaper’, ‘newspaper and book’, ‘book and note’, ‘note and book’ and ‘note and newspaper’. In this case the three words are assigned to a category. Note that a pair of nodes connected ... Discovery of Patterns Our first step is the discovery of patterns that are useful for lexical category acquisition. We use two main stages: discovery of pattern candi...

Ngày tải lên: 23/03/2014, 18:20

... on the results of the MS and sugar component analyses, pN4 was estimated to comprise three methyl mannoses, two mannoses and two N-acetylglucosamines, and the linkage position of the O-methyl ... diam- eter of the circles is proportional to the cube root of the detected amount of PA-glycans. The positions of standard PA-glycans are indicated by crosses labeled (a)–(g). The...

Ngày tải lên: 28/03/2014, 23:20