Báo cáo khoa học: Differential binding of factor XII and activated factor XII to soluble and immobilized fibronectin – localization of the Hep-1/Fib-1 binding site for activated factor XII pdf

... glycine–HCl, pH 2.2, at the end of each binding cycle. To measure the binding properties of Erbicin and ERB- hRNase to the extracellular domain of ErbB2, ECD was immobilized onto the surface of sensor chip ... competition in the case of ERB-hcAb. Effects of soluble ErbB2-ECD on the cytotoxicity of ERB-hcAb and Herceptin On the basis of the resul...

Ngày tải lên: 23/03/2014, 06:20

... implemented to help explain their importance to the specificity and catalytic efficiency of the enzyme. The role of the Phe-Phe entranceway The fortuitous discovery of an unreacted molecule of the mechanism-based ... b- glucan substrate in the )1 subsite at the bottom of the active site pocket [20]. The entrance to the active site pocket of Exg is fl...

Ngày tải lên: 15/03/2014, 23:20

... is sometimes observed in the second loop of the a/j-neuro- toxins and j-neurotoxins [22], where it specifically contri- butes to the binding of the toxins to the neuronal AChR [2 3–2 6]. Wntxs also possess ... potential prototype of Wntxs from cobras. Therefore, we decided to synthesize Wntx-5 for the investigation of biological properties of cobra Wntxs. Sy...

Ngày tải lên: 21/02/2014, 03:20

... transformed respectively with YCp-TRP 1– yHSF, YCp-TRP1–P ADH1 –hHSF2–T ADH1 , YEp-LEU 2– P ADH1 –hHSF2–T ADH1 , YCp-TRP1–P ADH1 –hHSF4b–T ADH1 , and YEp-LEU2–P ADH1 –hHSF4b–T ADH1 , and the resident YCp-URA3–yHSF ... 100 ng of hHSF–VP16 expression plasmid, and sufficient carrier pcDNA3.1(+) to bring the total amount of DNA to 1.6 lg. Cells were cultured for 2 0–2 4...

Ngày tải lên: 07/03/2014, 00:20

... series of gene duplications, fol- lowed by their sequence divergence, led to the diversity of the iLBP multigene family [3]. Previously, FABP ⁄ Fabps and their genes were named on the basis of the ... show differential tissue-specific distribution of fabp10a and fabp10b transcripts in developing and adult zebrafish, evidence of the divergence of regula- tory elem...

Ngày tải lên: 16/03/2014, 00:20

... of the protein–protein inter- action between v-KIND and MAP2. We defined the binding core regions for the v-KIND–MAP2 interac- tion and showed that the MAP2 binding core is not only critical for ... in the cell lysates. Taken together, these data indicate that the MAP2- binding core site of v-KIND is sufficient for the v-KIND-MAP2 interaction within cells. T...

Ngày tải lên: 14/02/2014, 19:20

... addi- tion to the Mxi1-WR isoform, other Mxi1 protein iso- forms exist both in mouse and man [1 2–1 4]. Many of these isoforms appear to arise from alternative exon 1 (and promoter) usage within the mxi1 ... these two isoforms. In the present study, we have compared further the Mxi1-SRa and Mxi1-SRb isoforms at the levels of Myc antagonism in the REF assay, subcell...

Ngày tải lên: 18/02/2014, 16:20

... Fig. 5). On the other hand, the concentra- tions for half-maximal inhibition (IC 50 ) of BPA were 35.7 nm for (275Ala)-ERRc, 27% of the binding affinity for the wild-type ERRc, and 990 nm for (316Ala)-ERRc, ... basal activity of ERRc due to its strong binding, but that its binding to the mutant receptors is not suf- ficient to keep their conformation in a f...

Ngày tải lên: 18/02/2014, 16:20

... acclimation to low (5 °C) and high (18 °C) temperatures leads to differential expression of alternative forms of the LDH-A gene in white skeletal muscle of weatherfish, Misgurnus fossilis. Two isoforms of ... have to be identified. Also, for a more detailed understanding of the functional and metabolic consequences, further study needs to identify the kinetic, th...

Ngày tải lên: 19/02/2014, 02:20

... the bionucleophiles DNA and GSH. The covalent binding of the drug to GSH reduced the formation of DNA adducts and slightly decreased the cytotoxic potential of the molecule. There is now little room for doubt ... residues at the N2 position in double- stranded DNA [11]. The covalent binding to DNA is apparently responsible for the cytotoxic action [12]...

Ngày tải lên: 20/02/2014, 11:20