Báo cáo khoa học: Differential binding of factor XII and activated factor XII to soluble and immobilized fibronectin – localization of the Hep-1/Fib-1 binding site for activated factor XII pdf
Báo cáo khoa học: Differential binding of human immunoagents and Herceptin to the ErbB2 receptor ppt
... glycine–HCl, pH 2.2, at the end of each binding cycle. To measure the binding properties of Erbicin and ERB-hRNase to the extracellular domain of ErbB2, ECD was immobilized onto the surface of sensor chip ... competitionin the case of ERB-hcAb.Effects of soluble ErbB2-ECD on the cytotoxicity of ERB-hcAb and HerceptinOn the basis of the results discussed above, the antitumour effects of ERB-hcAb and Herceptin ... Effects of soluble ErbB2-ECD on the binding and cytotoxic-ity of ERB-hcAb and Herceptin. Binding curves of ERB-hcAb (A) and Herceptin (B) to SKBR3 cells obtained by ELISA performed in the absence...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
... implemented to help explain their importance to the specificity and catalytic efficiency of the enzyme. The role of the Phe-Phe entranceway The fortuitous discovery of an unreacted molecule of the mechanism-based ... b-glucan substrate in the )1 subsite at the bottom of the active site pocket [20]. The entrance to the active site pocket of Exg is flanked by a pair of phenylalanyl resi-dues, Phe144 and Phe258 (both ... side chain B -factor (A˚2)24221917Average ligand B -factor (A˚2) BGC1a24Average ligand B -factor (A˚2) BGC2b75Average ligand B -factor (A˚2) L3-1c38Average ligand B -factor (A˚2)...
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Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx
... issometimes observed in the second loop of the a/j-neuro-toxins and j-neurotoxins [22], where it specifically contri-butes to the binding of the toxins to the neuronal AChR[2 3–2 6]. Wntxs also possess ... potential prototype of Wntxs from cobras. Therefore, we decided to synthesizeWntx-5 for the investigation of biological properties of cobra Wntxs.Synthesis and purification of synthetic Wntx-5 ... 6 2–6 8 amino acids and belong to the structural family of Ôthree-fingeredÕ folded toxins, whichincludes the cardiotoxins, muscarinic toxins, acetylcholin-esterase inhibitors and the a-neurotoxins...
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Báo cáo khoa học: Differential recognition of heat shock elements by members of the heat shock transcription factor family ppt
... transformed respectively with YCp-TRP 1– yHSF, YCp-TRP1–PADH1–hHSF2–TADH1, YEp-LEU 2– PADH1–hHSF2–TADH1, YCp-TRP1–PADH1–hHSF4b–TADH1, and YEp-LEU2–PADH1–hHSF4b–TADH1, and the residentYCp-URA3–yHSF ... 100 ng of hHSF–VP16 expression plasmid, and sufficient carrierpcDNA3.1(+) to bring the total amount of DNA to 1.6 lg.Cells were cultured for 2 0–2 4 h following transfection, and firefly and Renilla ... shocktranscription factors functionally substitute for yeastHSF. EMBO J 16, 646 6–6 477.29 Perisic O, Xiao H & Lis JT (1989) Stable binding of Drosophila heat shock factor to head -to- head and tail- to- tail...
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Báo cáo khoa học: Differential tissue-specific distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebrafish (Danio rerio) docx
... series of gene duplications, fol-lowed by their sequence divergence, led to the diversity of the iLBP multigene family [3].Previously, FABP ⁄ Fabps and their genes werenamed on the basis of the ... show differential tissue-specific distribution of fabp10a and fabp10b transcripts in developing and adult zebrafish, evidence of the divergence of regula-tory elements in the promoters of the fabp10a ... organizationcommon to all members of the iLBP multigene familyin vertebrates [1]. The sizes of each of the three introns of this duplicated fabp10b were 353, 375 and 258 bp,respectively. Each of the intron...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... of the protein–protein inter-action between v-KIND and MAP2. We defined the binding core regions for the v-KIND–MAP2 interac-tion and showed that the MAP2 binding core is notonly critical for ... in the cell lysates.Taken together, these data indicate that the MAP2- binding core site of v-KIND is sufficient for the v-KIND-MAP2 interaction within cells. The MAP2 -binding core module of ... thatresidues 70 2–7 44 of the smallest construct CD2-1-6-2contain the core sequence for v-KIND binding. To evaluate the v-KIND KIND2 -binding specificity of residues 70 2–7 44 of MAP2, we performed a pull-down...
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Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt
... addi-tion to the Mxi1-WR isoform, other Mxi1 protein iso-forms exist both in mouse and man [1 2–1 4]. Many of these isoforms appear to arise from alternative exon 1 (and promoter) usage within the mxi1 ... these two isoforms. In the presentstudy, we have compared further the Mxi1-SRa and Mxi1-SRb isoforms at the levels of Myc antagonism in the REF assay, subcellular localization, and transcrip-tional ... localize to the nucleus. (A) Schematic representation of the constructs used for the immunolocalization experiments shown in (B) and (C). Note that the Myc-tagged isoforms carry the tag on their...
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Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx
... Fig. 5). On the other hand, the concentra-tions for half-maximal inhibition (IC50) of BPA were35.7 nm for (275Ala)-ERRc, 27% of the binding affinity for the wild-type ERRc, and 990 nm for (316Ala)-ERRc, ... basal activity of ERRc due to its strong binding, but that its binding to the mutant receptors is not suf-ficient to keep their conformation in a fully activated form. The Arg316 fi Ala and Glu275 ... example, the phenol-hydroxyl group of BPA is tethered by hydrogenbonds to the Glu275 and Arg316 residues in the ERRc-LBD (Fig. 2). For a better understanding of the basal binding potentials to capture...
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt
... acclimation to low (5 °C) and high (18 °C) temperatures leads to differential expression of alternative forms of the LDH-A gene in white skeletal muscle of weatherfish, Misgurnus fossilis.Two isoforms of ... have to be identified. Also, for a more detailed understanding of the functional and metabolic consequences, further study needs to identify the kinetic, thermodynamic and regulatoryproperties of ... in their 3¢-UTR sequence binding sites for specific proteins that increase ⁄ decrease the rate of poly(A) shortening, i.e. affect the lifetime of the mRNA [32], therefore it is likely that the...
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Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc
... the bionucleophiles DNA and GSH. The covalent binding of the drug to GSH reduced the formation of DNA adducts and slightly decreased the cytotoxic potential of the molecule. There is now little room for doubt ... residues at the N2 position in double-stranded DNA [11]. The covalent binding to DNA isapparently responsible for the cytotoxic action [12] and the capacity of the drug to trigger apoptosis in ... Indirectly, these experimentssupport the idea that the formation of drug–DNA covalentcomplexes is responsible for the cytotoxic action [12].Finally, we evaluated the effect of BSO on the formationof...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roBT Tieng anh 6 UNIT 2Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ