Báo cáo khoa học: Creating hybrid proteins by insertion of exogenous peptides into permissive sites of a class A b-lactamase doc

... three-dimensional structure of TEM-1 is well characterized [7–9]. Like all class A b-lactamases, TEM-1 folds into a structure formed by an a ⁄ b-domain and an all -a- domain (Fig. 1A) . At the junction ... structures of many naturally occur- ring proteins reveal that they are composed of separate domains arising from the insertion of a new stretch of coding sequenc...

Ngày tải lên: 30/03/2014, 02:20

... beneficial. Because ANGPTL2 promotes vascular inflammation via the a5 b1 integrin ⁄ Rac1 ⁄ NF-jB pathway [15] and vascular injury accompanied by inflammation is con- sidered an early feature of arteriosclerosis ... Y, Akao M, Yasunaga K, Yamauchi T, Morisada T, Ito Y, Urano T, Kimura Y, Kubota Y, Maekawa H et al. (2005) Angiopoietin-related growth factor antago- nizes obesity and insulin re...

Ngày tải lên: 14/02/2014, 22:20

... polyoma- virus capsid proteins that serve as nuclear localization signals. Trans Kans Acad Sci 96, 35–39. 5 Moreland RB & Garcea RL (1991) Characterization of a nuclear localization sequence ... VP3– EGFP, and to determine the role of the mitochondrial pathway in apoptosis, cleavage of caspase 9 was investi- gated. Figure 5D shows caspase 9 cleavage (resulting in the appearance...

Ngày tải lên: 16/02/2014, 09:20

... decrease the stability of the canavany- lated species. The role of tRNA as a cofactor for aminoacylation in those aminoacyl-tRNA synthetases that require tRNA for amino acid activation is well documented [9], ... Preparation and colorimetric analysis of l-canavanine. Anal Biochem 77, 147–151. 60 Ozinskas AJ & Rosenthal GA (1986) l-Canavanine syn- thesis by zinc-mediated guanidin...

Ngày tải lên: 18/02/2014, 13:20

... phosphorylation, are activated and bind to a common mediator Smad (Co-Smad, Smad4). The complexes move into the nucleus and act as regulators of transcription. The activity of BMP is regulated by the ... transcripts are found in the organizer of gastrula embryos, later in the prechordal plate and axial mesoderm (Fig. 2). By contrast, noggin2 tran- scripts are detected at the end...

Ngày tải lên: 19/02/2014, 00:20

... major adduct of the anticancer drug cisplatin. Nature 377, 649–652. 29 Zlatanova J, Yaneva J & Leuba SH (1998) Proteins that specifically recognize cisplatin-damaged DNA: a clue to anticancer ... Ichimiya S, Nakagawara A, Sakuma Y, Kimura S, Ikeda T, Satoh M, Takahashi N, Sato N & Mori M (2000) p73: structure and function. Pathol Int 50, 589–593. 23 Levrero M, De Laurenzi V, Cos...

Ngày tải lên: 19/02/2014, 05:20

... 745–751. 29 Osanai K, Takahashi K, Nakamura K, Takahashi M, Ishigaki M, Sakuma T, Toga H, Suzuki T & Voelker DR (2005) Expression and characterization of Rab38, a new member of the Rab small G protein ... membrane, most of these mitochondrial pro- teins behave as if they have a- helical transmembrane domains, rather than b-barrels. These proteins are usually predicted to have...

Ngày tải lên: 19/02/2014, 07:20

... boundaries as hidden variables and include probabilities for let- ter transitions within segments. The ad- vantage of this model family is that it can learn from small datasets and easily gen- eralises ... terms of training set size. We want to remind the reader that our two algorithms are aimed at small datasets. We randomly split each dataset into 10 subsets where each subset was a...

Ngày tải lên: 20/02/2014, 04:20

... bedrag- gled 56-year-old [professor]. Also, in a particu- lar domain, words may have a non-standard usage. Systems trained on labeled data can learn the do- main usage and leverage other regularities, ... evaluate the benefit of N-gram data on multi- class classification problems. For each task, we have some labeled data indicating the correct out- put for each example. We evaluate with...

Ngày tải lên: 20/02/2014, 04:20

... Lee and Seneff, 2008; Nagata et al., 2004; Nagata et al., 2005; Nagata et al., 2006; Tetreault et al., 2010b). This is one of the most active research areas in natural language processing of learner ... corpus Ryo Nagata Konan University 8-9-1 Okamoto, Kobe 658-0072 Japan rnagata @ konan-u.ac.jp. Edward Whittaker Vera Sheinman The Japan Institute for Educational Measurement Inc. 3-2-4 Kit...

Ngày tải lên: 20/02/2014, 04:20