Báo cáo khoa học: Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 docx

... Journal compilation ª 2009 FEBS Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 Hugo M. Botelho 1 , Michael Koch 2 ,Gu ¨ nter Fritz 2 and Cla ´ udio M. Gomes 1 1 ... further explore the interplay between Zn 2+ and Ca 2+ binding to S100A2 and to address how metallation affects the protein conformation and stability...

Ngày tải lên: 30/03/2014, 02:20

... mice [28]. The instability of DA at the neutral pH of the cytosol causes the forma- tion of toxic aminochromes, and these species are not formed when DA is protonated at the acidic pH of the storage ... enriched in the light fraction LP2, together with the pre-synaptic vesicle marker synaptophysin. Lower concentrations of DAT were present in the vesicular fractio...

Ngày tải lên: 14/02/2014, 21:20

... [23], a peptide from the Win motif of MLL1 bound to the WD40 repeat protein, WDR5 [24,25] and the C-terminal SET domain in the presence and absence of histone peptides and the cofactor product, S-adenosyl-homocysteine ... coordinates two zinc ions using the two conserved CGXCXXC motifs (Fig. 2A). The zinc ions are required for the structural integrity of the...

Ngày tải lên: 16/02/2014, 14:20

... maintaining the homeostasis of key metal ions within the body. Like other MT isoforms, GIF binds both monovalent and divalent metal ions with high affinity. They have the ability to inhibit heavy metal toxicity ... within the brain. Further studies are required to determine the function of GIF within the normal, injured and neurodegenerative brain, and to establ...

Ngày tải lên: 16/02/2014, 15:20

... almost linear and can be obtained by calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredthatthe formation of the N-terminal ... (Met1)-ONC (M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance of the (Met1...

Ngày tải lên: 19/02/2014, 12:20

... condi- tions). Figure 4B shows the X-loading weights of the X-variables (measured variables other than NADPH) and the Y-loading of NADPH on the two PCs. As NADPH is in the first quadrant of Fig. 4B (the ... [26]. Overproduction of 6PGDH resulted in an increase of synthesis of pyr- idine nucleotide cofactors, as the total pool of these increased. Whether the synth...

Ngày tải lên: 07/03/2014, 17:20

... kDa band) that the clearance and further metabo- lism of the allergen was altered as a result of the inflammation in the lungs of sensitized animals. Up to now there are few data available on the ... noticed. The pattern of labelled protein bands differed between the tissues. Only in lung a 16 kDa protein band was detected 24 h after i.t. administration of [ 75 Se...

Ngày tải lên: 07/03/2014, 21:20

... label the nucleus, and the cells were then fused to nontransfected murine 3T3 cells in the presence of a protein synthesis inhibitor, cyclohexi- mide, and further incubated for 1 h to see whether GFP- ... indicates that the translocation of the substrate–Trn-1 complex through NPCs to the nucleoplasmic side is independent of both Ran and energy and that RanGTP and...

Ngày tải lên: 07/03/2014, 21:20

... presents the Turker with the name and website of a hotel. The HIT instructions ask the Turker to assume that they work for the hotel’s mar- keting department, and to pretend that their boss wants them ... gen- eral. Moreover, they are potentially detectable via off -the- shelf plagiarism detection software. 310 of gold-standard data, based on the distortion of pop- ulari...

Ngày tải lên: 07/03/2014, 22:20

... expectations on the par~ of the user and the needs of the user. Within each type of interaction there can be many user types and the same infoz~nation may be needed by these different types of ... is said and how it is interpreted. The ~ separate aspects of this question that must be considered are the func- tion and the domain of the discourse. I. T...

Ngày tải lên: 17/03/2014, 19:20