Báo cáo khoa học: Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf

... Ca 2+ -binding motif in an LH1 complex FEBS Journal 276 (2009) 1739–1749 ª 2009 The Authors Journal compilation ª 2009 FEBS 1749 Specific Ca 2+ -binding motif in the LH1 complex from photosynthetic bacterium ... of LH1 from Tch. tepidum have been purified and the amino acid sequences deter- mined [12]. In addition, the dimeric feature and the highly...

Ngày tải lên: 30/03/2014, 02:20

... to date, and include the association with phospholipids and membranes [13–16], the inhibition of Raf1 kinase [17,18], thrombin [19], and G-protein-coupled receptor kinase 2 [20], and the N-terminal ... specific interactions, resulting in the higher affinity for PS, PtdIns(3)P, PtdIns(3,4)P 2 , and PtdIns(3,4,5)P 3 . Involvement of the CPY-binding sites of I C in its me...

Ngày tải lên: 19/02/2014, 05:20

... and hardly soluble in water, hydrophilic retinoid-binding proteins are therefore required for stabilizing retinoids in the watery plasma as well as in the cytoplasm, and for transporting retinoids within ... cellular retinal-binding protein; CRBP, cellular retinol-binding protein; IRBP, interphotoreceptor retinoid-binding protein; RBP, retinol-binding protein. Note: The nu...

Ngày tải lên: 17/03/2014, 03:20

... the changes in the thermal unfolding of actin induced by interaction of G-actin with actin-binding proteins [25– 27], G–F transformation of actin, and stabilization of F-actin by phalloidin and ... on the thermal unfold- ing of G- and F-actin. Previously, the thermal unfold- ing of G-actin containing different nucleotides was indirectly studied with the DNase-I inhibitio...

Ngày tải lên: 06/03/2014, 22:21

... specific cis-element and the structure for direction of a plus-RNA synthesis might be present in the minus-RNA, as well as i n the plus-RNA. To investigate the binding activity of the CSFV NS5B protein on the minus-RNA, ... dRp assays, and examined the in uence of d ifferent cis-acting elements on binding of NS5B protein and RNA synthesis in vitro. Materials and...

Ngày tải lên: 19/02/2014, 16:20

... by N-terminal amino-acid sequencing are indica- ted with red arrows; the blue underlines are the assumed cleavage sites based on molecular sizes and sequencing. The main digestion of the urease ... cleavage sites of the urease by N-terminal amino-acid sequencing of the peptide frag- ments. From the band (1), a sequence of GLIVT was detected with the intensity of 2 pmol...

Ngày tải lên: 07/03/2014, 21:20

... calcium– calmodulin-dependent protein kinase kinase has a role in utilizing glucose through interaction with AMP- dependent protein kinase [40], which is implicated in diabetes [41]. In S. pombe, Ssp2 is the ... mm). Glucose was nearly exhausted at the end of the experiment for the initial 4.4 mm glucose, and the dou- bling times from the cell number increase during the...

Ngày tải lên: 14/03/2014, 23:20

... Distinct regulation of osmoprotective genes in yeast and mammals. Aldose reductase osmotic response element is induced independent of p38 and stress-activated protein kinase ⁄ Jun N-terminal kinase ... DNA-binding specificity of the resulting proteins [39]. Alternative splicing of the transactivation domains in Pax-8 [40], the POU homeodomain family protein Pit-1 [41] and the...

Ngày tải lên: 30/03/2014, 10:20

... concentration. The dissociation constants, K d values, enthalpies of binding and stoichiometries were determined from the binding isotherm by fitting a 1 : 1 binding model to the data using the software ... This was interpreted as a lack of fosfomycin binding to these mutant proteins [12]. Binding of fosfomycin to either wild-type MurA or the K22 mutant proteins in the ab...

Ngày tải lên: 19/02/2014, 13:20

... to 30 kDa in size. Subunits of all known GST structures exhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly conserved GSH-binding site (G-site) and the more ... Citrus sinensis GSTU1 (REI) and the PEI mutant, as well as for Citrus sinensis GSTU2 (PKV) and the RKV mutant, by submitting the amino acid sequences to swiss-model...

Ngày tải lên: 15/03/2014, 09:20