Báo cáo khoa học: Speciﬁc Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf

... Ca2+-binding motif in an LH1 complex FEBS Journal 276 (2009) 1739–1749 ª 2009 The Authors Journal compilation ª 2009 FEBS 1749 Speciﬁc Ca2+-binding motif in the LH1 complex from photosynthetic bacterium ... of LH1 from Tch. tepidum have been puriﬁed and the amino acid sequences deter-mined [12]. In addition, the dimeric feature and the highly symmetric ring assembly of BChls in LH1, as well as the ... isalso known as the EF hand [16]. Proteins containing the EF hand are divided into two classes accordingto their functions: signaling and buffering ⁄ transportproteins. The former undergoing Ca2+-dependentconformational...

Tài liệu Báo cáo khoa học: Speciﬁc membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc

... todate, and include the association with phospholipids and membranes [13–16], the inhibition of Raf1 kinase[17,18], thrombin [19], and G-protein-coupled receptorkinase 2 [20], and the N-terminal ... speciﬁc interactions, resulting in the higher afﬁnity for PS, PtdIns(3)P, PtdIns(3,4)P2, and PtdIns(3,4,5)P3.Involvement of the CPY-binding sites of IC in itsmembrane binding As IC–CPY has ... endogenous cytoplasmic inhibitor of vacuolar carboxypeptidase Y in the yeast Saccharomyces cerevisiae, is classiﬁed as a member of the phos-phatidylethanolamine-binding protein family. The binding of...

Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterﬂy, Papilio xuthus docx

... and hardly soluble in water, hydrophilic retinoid-binding proteins are thereforerequired for stabilizing retinoids in the watery plasma as well as in the cytoplasm, and for transporting retinoidswithin ... cellular retinal-binding protein; CRBP,cellular retinol-binding protein; IRBP, interphotoreceptorretinoid-binding protein; RBP, retinol-binding protein.Note: The nucleotide sequence reported in this ... RBPbinds the ligand ubiquitously. When the eyes were light-adapted, however, the amount of RBP, as well as the ﬂuorescence of the ligand, increased in the distal portion and decreased in the...

Báo cáo khoa học: Speciﬁc cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot

... the changes in the thermal unfolding of actin induced by interaction of G-actin with actin-binding proteins [25–27], G–F transformation of actin, and stabilization ofF-actin by phalloidin and ... on the thermal unfold-ing of G- and F-actin. Previously, the thermal unfold-ing of G-actin containing different nucleotides wasindirectly studied with the DNase-I inhibition assay[23] and by ... participating both in the intra-strand contacts between actin subunits along the ﬁlament and in the lateral contacts stabilizing the inter-strand interaction, whereas the C-terminal part of the loop...

Tài liệu Báo cáo khoa học: Speciﬁc interaction between the classical swine fever virus NS5B protein and the viral genome pdf

... speciﬁc cis-element and the structure for direction of a plus-RNAsynthesis might be present in the minus-RNA, as well as i n the plus-RNA. To investigate the binding activity of the CSFV NS5B protein on the minus-RNA, ... dRpassays, and examined the in uence of d ifferent cis-actingelements on binding of NS5B protein and RNA synthesis in vitro.Materials and methodsExpression and puriﬁcation of NS5B proteins The ... and binds the 3¢ UTRandstartsRNAsynthesis,inwhichaminus-strand RNA is produced with the plus-strandgenomic RNA as a template. Then, a progeny plus-RNAis produced with the novel minus-RNA as...

Báo cáo khoa học: Speciﬁc degradation of H. pylori urease by a catalytic antibody light chain pptx

... by N-terminal amino-acid sequencing are indica-ted with red arrows; the blue underlines are the assumed cleavage sites based onmolecular sizes and sequencing. The maindigestion of the urease ... cleavage sites of the urease by N-terminal amino-acid sequencing of the peptide frag-ments. From the band (1), a sequence of GLIVT wasdetected with the intensity of 2 pmol. As a minor scis-sile ... E124-G125.On the other hand, band 2 gave three main sequences:GLIVT (0.9 pmol), AINHA (0.9 pmol), and DVQVA(0.8 pmol). The ﬁrst one was identical to the N-ter-minal sequence of the main band (1), and...

Báo cáo khoa học: Speciﬁc biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in ﬁssion yeast docx

... calcium–calmodulin-dependent protein kinase kinase has a role in utilizing glucose through interaction with AMP-dependent protein kinase [40], which is implicated in diabetes [41]. In S. pombe, Ssp2 is the ... mm).Glucose was nearly exhausted at the end of the experiment for the initial 4.4 mm glucose, and the dou-bling times from the cell number increase during the earlier period (4–8 h, 3.0 mm remaining ... thus increased the lifespan by approximately 10times under the fasting condition. These remarkableﬁndings of a lifespan increase under fasting conditions by previous starvation were further investigated...

Báo cáo khoa học: Speciﬁc TSC22 domain transcripts are hypertonically induced and alternatively spliced to protect mouse kidney cells during osmotic stress pptx

... Distinct regulation of osmoprotective genes in yeast and mammals. Aldose reductase osmoticresponse element is induced independent of p38 and stress-activated protein kinase ⁄ Jun N-terminalkinase ... DNA-binding speciﬁcity of the resulting proteins[39]. Alternative splicing of the transactivationdomains in Pax-8 [40], the POU homeodomain familyprotein Pit-1 [41] and the zinc ﬁnger transcription ... functionally distinctisoforms from a single gene by use of different combi-nations of splice junctions. For example, alternativesplicing within the DNA-binding domain of Pax-6alters DNA-binding speciﬁcity...

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... concentration. The dissociation constants, Kdvalues, enthalpies of binding and stoichiometries weredetermined from the binding isotherm by ﬁtting a 1 : 1binding model to the data using the software ... This was interpreted as a lack of fosfomycinbinding to these mutant proteins [12]. Binding of fosfomycinto either wild-type MurA or the K22 mutant proteins in the absence of UDPNAG is not associated ... confor-mation) whereas in the liganded form (ÔclosedÕ conforma-tion) a loop in the upper domain forms a lid on the activesite, thereby shielding the ligands from solvent and gener-ating a compact...

Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf

... to30 kDa in size. Subunits of all known GST structuresexhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly conservedGSH-binding site (G-site) and the more ... Citrus sinensis GSTU1 (REI) and the PEI mutant, as well as for Citrus sinensis GSTU2 (PKV) and the RKV mutant, by submitting the amino acid sequencesto swiss-model [35]. Then, the alignments of the ... analog and with the product reveals two completely differentbinding modes for the intermediate and the product,suggesting that a speciﬁc motion is associated with the collapse of the intermediate...

Xem thêm