Báo cáo khoa học: Channel-forming activities of peroxisomal membrane proteins from the yeast Saccharomyces cerevisiae pot

... ª 2009 The Authors Journal compilation ª 2009 FEBS Channel-forming activities of peroxisomal membrane proteins from the yeast Saccharomyces cerevisiae Silke Grunau 1,2, *, Sabrina Mindthoff 1, *, ... peroxisomes from the yeast Saccharomyces cerevisiae grown on oleic acid were investigated for the presence of channel (pore)-forming proteins in the mem...

Ngày tải lên: 30/03/2014, 02:20

... Oxidative stress response in the yeast Saccharomyces cerevisiae. Yeast 14, 1511–1527. 22 Moradas-Fereira P & Costa V (2000) Adaptive response of the yeast Saccharomyces cerevisiae to reactive oxygen species: ... that, at least in part, the Ca 2+ flux comes from outside the cells. Following the addition of BAPTA, the pattern of the luminescence spectrum re...

Ngày tải lên: 15/03/2014, 23:20

... PCR’, using the first PCR product as template. To make use of the BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product of the nes- ted PCR was replaced with the corresponding ... isozymes, and that the degradation of TFM is less sensitive to alterations of these residues than is the degrada- tion of physiological substrates. These results sup...

Ngày tải lên: 07/03/2014, 05:20

... interfere with the pyrimid- ine nucleotide pools of this pathogen. All DHODH proteins of family 2 must be translo- cated from the cytosol to the inner membrane of mito- chondria. The proteins are ... showed that the purified enzymes were of the expected molecular mass of 48 kDa for the full-length enzyme and 42 kDa for the truncated enzyme. The yield of re...

Ngày tải lên: 07/03/2014, 12:20

... regarding the importance of the pro- nounced secondary structure in this region. Mutation of either proline could result in reduction or increase in the length of the helical secondary structure of the ... facing the inside of the cell. Five of the residues lie within the previously characterized N-terminal regulatory domain and two mutations are located within...

Ngày tải lên: 07/03/2014, 15:20

... as the number of web pages containing the words the and ’and’. 891 matched. On the basis of these, we then calculate the probability of a certain qualia element given a certain role on the ... clue c, the query c(n) is sent to the web search engine and the abstracts of the first m docu- ments matching this query are downloaded. Then the snippets are processed to find...

Ngày tải lên: 08/03/2014, 02:21

... 8.5, we studied thermal stability of 17b-HSDcl from CD and UV melting curves, from which the temperature of denaturation (T d ) and the van’t Hoff enthalpy of denaturation (DH° vH )of1 7b-HSDcl were ... Inspection of Fig. 2B reveals two transitions, the first in the pH range pH 7–5, and the second in the pH range 5–3. The observed transitions in the aci- dic pH ran...

Ngày tải lên: 16/03/2014, 13:20

... examine the topology of the binding site of the anti-MP8 Ig, 3A3, especially to evaluate the size of the cavity remaining around the iron atom. For this purpose, we studied the formation of Fe(II)-nitrosoalkane ... regioselectivity of the reaction in favor of the formation of 2-nitrophenol [3]. Consequently, it was tempt- ing to examine whether the hemoabzyme...

Ngày tải lên: 19/02/2014, 12:20

... their membranes, and thus the lack of a specific molecular microbial target minimizes resistance development. The fundamental principle of action of most peptides depends on the ability of these molecules ... concentrations of 10 l M to  1000 l M and at higher concentrations the peptide preci- pitated. Thus, the index expresses the level of antibacterial activity in re...

Ngày tải lên: 07/03/2014, 15:20

... arms of the replication fork, the lagging and the leading strand, respectively [61,64]. We therefore favour the idea that physical uncoupling of the leading strand and the lagging strand DNA synthesis may ... Analysis of the essential functions of the C-terminal protein ⁄ protein interaction domain of Saccharomyces cerevisiae pol e and its unexpected ability to s...

Ngày tải lên: 30/03/2014, 10:20