Báo cáo khoa học: X-ray crystallographic and enzymatic analy

Báo cáo khoa học: X-ray crystallographic and enzymatic analyses of shikimate dehydrogenase from Staphylococcus epidermidis pot

... structures of Staphylococcus epidermidis SDH (SeSDH) both in its ligand-free form and in complex with shikimate. SeSDH has a k cat of 22.8 s )1 and a K m of 73 lm towards shikimate, and a K m of 100 ... from S. epidermidis FEBS Journal 276 (2009) 1125–1139 ª 2009 The Authors Journal compilation ª 2009 FEBS 1139 X-ray crystallographic and enzymatic analyses o...

Ngày tải lên : 30/03/2014, 02:20

15
308
0

Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... expressed, and puriﬁed in Escherichia coli system. The enzymatic characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm toward shikimate, k cat of 7.1 s )1 and ... properties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028 mm and k...

Ngày tải lên : 19/02/2014, 05:20

11
529
0

Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... lysozyme. This hypothesis is not con- ﬁrmed by the present analysis of enzymatic properties of ChiG, or by our analysis of the ChiG and ChiC structures. Despite the deletion of the B-loop, the ... that catalyze the hydrolysis of chitin, a carbohydrate poly- mer of 1,4-b-linked GlcNAc. Chitin is found in the cuticle of insect shells, in shells of crustaceans, and in the c...

Ngày tải lên : 07/03/2014, 11:20

12
399
0

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... human and pufferfish (torafugu and spotted green pufferfish) IL-10 sequences. Pufferfish and carp IL-10 genes, clustered together and distant from IL-20 and IL- 24, as recently determined from analysis ... (AAG41401), IL-26 (NP_060872) and interferon-a (P01579); from torafugu IL-10 (CAD62446); from spotted green pufferfish IL-10 (CAD 67773), IL-20 (AY294557) and IL-24 (AY2945...

Ngày tải lên : 20/02/2014, 02:21

8
584
0

Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai Ken-ichi Suzuki, Takao Ojima and Kiyoyoshi Nishita Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries ... phosphate (pH 7.0), and an appropriate amount of enzyme at 30 °C. The reducing sugar liberated by hydrolysis of CMC was determined by the method of Nelson and So...

Ngày tải lên : 20/02/2014, 23:20

8
511
0

Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... ﬂuorescence intensity as function of time of double- labeled liposomes made from PS (A) or from PC (B) after the addition of MfGl-IIorLPSReatt = 50 s and subsequent addition of LBP (0.2 m M )att = 100 ... were kind gifts of K H. Wiesmu ¨ ller 1 (Tu ¨ bingen, Germany). Bovine brain 3-sn-PS and egg 3-sn-PC were obtained from Sigma (Deisenhofen, Germany). For pre- paration o...

Ngày tải lên : 21/02/2014, 00:20

9
665
1

Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of ﬂavonol synthase from Citrus unshiu pptx

... evolved from a common ancestral gene, and the essence of most of the conserved amino acids has been further substantiated by site-directed mutagenesis of FHT [7,9] and by documentation of ligand ... substrate (dihydroquercetin) and product (quercetin) were spotted by absorbance at 366 nm, and the product was extracted with methanol. The solution was ﬁltered and concentrate...

Ngày tải lên : 21/02/2014, 03:20

9
864
0

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... graph of R 1 , R 2 and { 1 H}– 15 N NOE val- ues along the sequence of the TTF1 HD at 11.7 T and 286 K. Fig. S2. Plot of J(0.87x N )–J(0) correlation from 15 N relaxation measurements of the ... and Ala60–Gln67), together with those from Arg58 and Gln59 in the C-terminal end of helix III, are located apart from the major cluster, towards the lower intercept of the th...

Ngày tải lên : 18/02/2014, 16:20

14
744
0

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. It ... structure of conomarphin was similar to those of other conotoxin cDNAs. The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 re...

Ngày tải lên : 18/02/2014, 17:20

12
616
0

Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elaﬁn) do not depend on its protease inhibitory function pptx

... nature of the molecule, as assessed from the salt and heparin dependence of the antimicrobial and antifungal effects. Results Antimicrobial effects of recombinant wild-type trappin-2 and trappin-2 ... (2006) Secretion of serine peptidase by a clinical strain of Candida albicans: inﬂuence of growth conditions and cleavage of human serum proteins and extracellular ma...

Ngày tải lên : 18/02/2014, 17:20

13
610
0