Báo cáo khoa học: Polyphosphates from Mycobacterium bovis

Báo cáo khoa học: Polyphosphates from Mycobacterium bovis – potent inhibitors of class III adenylate cyclases pptx

... inhibition of adenylate cyclases FEBS Journal 276 (2009) 109 4–1 103 ª 2009 The Authors Journal compilation ª 2009 FEBS 1103 Polyphosphates from Mycobacterium bovis – potent inhibitors of class III adenylate ... polyphosphates with a mean chain length of 72 residues as highly potent inhibitors of dimeric class IIIa, class IIIb and class IIIc ACs...

Ngày tải lên : 30/03/2014, 02:20

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Báo cáo khoa học: EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein pptx

... char- acteristics of EspB are those of a natively partially folded protein. The far-UV CD spectra of IpaC, a homolog of EspB from Shigella ﬂexneri, revealed an absence of signiﬁcant amounts of secondary ... 75 6–7 68 ª 2005 FEBS disordered. The results from PONDRÒ suggest that the amino acid residues at 1–5 3, 12 8–2 30 and 24 7–2 87 may be disordered. This is relative...

Ngày tải lên : 16/03/2014, 18:20

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Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx

... vary from cell to cell. In some cell types, particularly (but not uniquely) phagocytes [11,1 3–1 9], reduction of the steady-state level of lysoPC slows the initial rate of incorporation of exogenous ... University of Valladolid School of Medicine, Spain The phospholipase A 2 (PLA 2 ) superfamily is a hetero- geneous group of enzymes with distinct roles in cell function [ 1...

Ngày tải lên : 18/02/2014, 17:20

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Báo cáo khoa học: Halogenated benzimidazoles and benzotriazoles as inhibitors of the NTPase/helicase activities of hepatitis C and related viruses ppt

... (IC 50 of 1. 5–5 .5 l M ) of the helicase activities of HCV, WNV and Suv3(D 1–1 59), but not JEV. The tetrachlorobenzotriazole TCBT is only a weak inhibitor of the HCV enzyme, and a moderate inhibitor of the ... N-terminal region of Suv3(D 1–1 59) NTPase/helicase is highly homologous to domains I and II, but not III, of HCV NTPase/helicase. Consequently, it is of intere...

Ngày tải lên : 08/03/2014, 02:20

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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot

... (µ M) 51015 0.30 0.25 0.20 0.15 0.10 0.05 0.00 0.5 0.4 0.3 0.2 0.1 0.0 –2 0 –1 0 –2 0 0204060 0102030 Efavirenz (n M) Efavirenz ( nM ) 1/n (fmoles of product)1/n (1/fmoles of product)1/n (1/fmoles of product) Fig. 2. Yonetani–Theorell plot of the interaction ... using the Yonetani–Theorell plot, the slope and intercepts of the two plots of 1 ⁄ v versus efavirenz concentr...

Ngày tải lên : 22/03/2014, 16:20

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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... A 340 as a result of NADH oxidation was measured. One unit of enzyme activity was deﬁned as the reduction of 1 lmolÆmin )1 of NADH (corresponding to the carboxylation of 1 lmolÆmin )1 of acetyl-CoA). Quantiﬁcation ... addition of CoA solution). The K m value for CoA of the OGOR enzyme is reported to be 80 lm [30]. Thus, decarboxyl- ation of 2-oxoglutarate can proceed as a re...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide doc

... of AChE secreted into media are G 2 a and Substrate (M) 10 –6 10 –5 10 –4 10 –3 10 –2 10 –1 10 –7 Cholinesterase activity (mAb·min –1 ) 0 200 400 600 800 1000 1200 Fig. 3. Representative experiment ... catalytic gorge of C. intestinalis AChE is similar to that of vertebrate AChEs, showing Fractional AChE activity 1.00 0.75 0.50 0.25 0.00 Inhibitor ( M) 10...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

... C-terminal was not cleaved off. The bridges are formed by residues 6 6–9 8 and 16 7–1 98 in SPRK, 6 7–9 9, 16 3–1 94 and 27 7– 281 in VPRK (VPRK numbering) and 3 4–1 23 and 17 8–2 49 in PRK. One of the disulﬁde bridges ... Faculty of Science, University of Tromsø, Tromsø, Norway 2 Department of Molecular Biotechnology, Faculty of Medicine, University of Tromsø, Trom...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx

... belonging to the family of glutathione reductase [8], of which FprA is a member. The FprA monomer consists of two domains: the FAD-binding domain (residues 2–1 08 and 32 4–4 56) consisting of the N- and C-terminal regions ... intensity of FAD corresponds to the release of protein-bound FAD on denaturation [12,13]. Hence, the possibility of GdmCl-induced release of FAD fr...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Si-face stereospeciﬁcity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt

... stereoselectivity of enzymatic transfer of hydrogen from nicotinamide cofactors. J Am Chem Soc 105, 588 6–5 890. 11 Berk H, Buckel W, Thauer RK & Frey PA (1996) Re-face stereospeciﬁcity at C4 of NAD(P) ... that of a mixture of [5- 1 H]F 420 and [5- 2 H]F 420 (c). The simulated stick spectrum of reduced F 420 (b, white) and that of the FprA oxidation product (c, white) ar...

Ngày tải lên : 20/02/2014, 03:20

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