Báo cáo khoa học: Biochemical and molecular characterization

Báo cáo khoa học: Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2 docx

... ª 2009 FEBS Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A 2 Aida Karray, Fakher Frikha, Abir Ben Bacha, Yassine Ben Ali, Youssef Gargouri and Soﬁane ... zymogen of phospholipase A in human pancreatic juice. Biochim Biophys Acta 227, 213–217. 12 Evenberg A, Meyer H, Verheij HM & de Haas GH (1977) Isolation and propertie...

Ngày tải lên : 30/03/2014, 01:20

10
358
0

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... better clarify the occurrence of LOXs and their inﬂuence on hazelnut seed quality, we carried out the biochemical and molecular characterization of the main LOX isoforms expressed during seed ... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main p...

Ngày tải lên : 21/02/2014, 00:20

11
404
0

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... localization and association with the development of fruiting bodies. J. Gen. Microbiol. 125, 147–157. 328 S. Chen et al. (Eur. J. Biochem. 271) Ó FEBS 2003 Biochemical and molecular characterization of ... °C. Enzyme characterization The molecular mass of puriﬁed laccase was determined by SDS/PAGE (15% w/v acrylamide gels) using low molecular mass standards (Bio-Rad)....

Ngày tải lên : 07/03/2014, 15:20

11
703
0

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... standard molecular weight proteins. The ﬂow rate was set to 0.8 mL Æmin )1 . The subunit molecular mass was also estimated by SDS–PAGE. Characterization and comparative analyses of HYD Js and ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate...

Ngày tải lên : 18/02/2014, 08:20

14
621
0

Báo cáo khoa học: Biochemical and spectroscopic characterization of the bacterial phytochrome of Pseudomonas aeruginosa pdf

... reaction. Here we describe the further biochemical and spectroscopic characterization of BphP. Results Expression, puriﬁcation and initial characterization of recombinant P. aeruginosa phytochrome The ... NaOH apo Pfr Pr protein 32 P Fig. 6. Autoradiogram of BphP. Autoradiogram after [ 32 P]ATP[cP] labeling, SDS ⁄ PAGE and electroblotting. (A) Autoradiogram of the apo a...

Ngày tải lên : 16/03/2014, 18:20

10
412
0

Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

... function of time and protein concentration. One unit of enzyme activity was deﬁned as the amount of enzyme that catalyzes the cleavage of 1 lmol of inosine per minute at 80 °C. Determination of kinetic ... respon- sible for the different substrate speciﬁcity of hPNP and hMTAP (Glu201, Asn243 and Val245 of hPNP instead of Ser178, Asp220 and Asp222 of hMTAP, respect-...

Ngày tải lên : 23/03/2014, 09:21

14
384
0

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... DNA and RNA were performed as described previously [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the ... al. Characterization of MAPEG members FEBS Journal 272 (2005) 1688–1703 ª 2005 FEBS 1699 BamHI and EcoRI and then the reformatted BamHI-EcoRI fragment was subcloned into BamHI a...

Ngày tải lên : 19/02/2014, 17:20

16
524
0

Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc

... respect to the composition of its secretion apparatus and the molecular details of its secretion mechanisms. This review describes the molecular properties of the Cag-T4SS and compares these with ... phosphorylation of its effector protein CagA into various host cells is a hallmark of Cag- T4SS activity. Despite the molecular characterization of a number of effects...

Ngày tải lên : 06/03/2014, 00:21

10
393
0

Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

... and GluTR, whose spectrum and DLS signal are shown in Fig. S11. The migration positions of GluTR and GluRS, were determined by comparison with those of a homogeneous sample of GluRS (S) and of ... subjected to TLC and quantiﬁcation of radiolabelled ADP and AMP. The kinetics of [ 3 H]AMP and [ 3 H]ADP formation in the absence of tRNA Glu (black symbols) or of both...

Ngày tải lên : 07/03/2014, 03:20

20
497
0

Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the ﬁrst ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt

... tetraloop out of which A20 of SRD RNA corresponding to the A4324 of rat ribosomal RNA was ﬂipped [27]. Lamjapin could deadenylate A20 of SRD RNAandreleasedtheRNAfragmentwiththeexactsizeof the R-fragment ... release more than one mole of adenine from each mole of ribosomes in 10 min and even up to 12 mol of adenines in 40 min (Fig. 4B). Base and position speciﬁcity of lam...

Ngày tải lên : 08/03/2014, 10:20

7
479
0