Báo cáo khoa học: Protein oligomerization induced by oleic acid at the solid–liquid interface – equine lysozyme cytotoxic complexes potx
Báo cáo khoa học: Protein oligomerization induced by oleic acid at the solid–liquid interface – equine lysozyme cytotoxic complexes potx
... ª 2009 The Authors Journal compilation ª 2009 FEBS 3989 Protein oligomerization induced by oleic acid at the solid–liquid interface – equine lysozyme cytotoxic complexes Kristina Wilhelm 1 , Adas ... display the minimum at 230 nm typical of the native state equine lysozyme spectrum at 25 °C, but exhibit the same shape as the spectrum for the...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf
... visfatin–PPREwt (5¢-CAAT AC AGGGCAAAGATCATGGAAG-3¢) or visfatin–PPRE- mut (5¢-CAATAC AGGAAAAAGAAAATGGAAG-3¢) oli- gonucleotides were added to the mixture 10 min before the DR1–visfatin–PPREwt. The ... (DR1–visfatin–PPREwt) 6 –TK–pGL3 was generated by inserting six copies of the double-strand oligonucleotides (forward, 5¢-CAATAC AGGGCAAAGAT CATGGAAG-3¢; and reverse, 5¢-CTTCCATGATCTTT...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
... congeners by demonstrating the activation of caspase-3, as a sensitive marker, using L-MAT as a model T-cell line [8]. The role played by the aromatic hydrocarbon recep- tor (AhR)-mediated pathway ... although the immediate target for PKCh-mediated phosphorylation in the SEK1 ⁄ JNK pathway is unknown [28]. Therefore, in TCDD -induced L-MAT cell apoptosis it is possible that PKC...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Protein engineering of pyruvate carboxylase Investigation on the function of acetyl-CoA and the quaternary structure doc
... disrupted by soni- cation and then centrifuged. The precipitate that formed was removed by centrifugation, and ammonium sulfate was added to the supernatant to 4 0–5 0% saturation for intact PC ... progress of the reaction was followed by monitoring the formation of ADP in the presence of phosphoenolpyru- vate and pyruvate kinase. The pyruvate formed was then reduced to la...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Cyclosporin A-induced oxidative stress is not the consequence of an increase in mitochondrial membrane potential doc
... done in state III respiration. DNP (final concentration 20 lm) and state IV respiration served as negative controls. Mitochondria were incubated for 15 min at 37 °C. At the end of the incubation period, ... MPTP by CsA altered the flow of electrons along the electron transfer chain, mitochondrial ATP synthesis and respiration rate would be expected to alter. Our results showed tha...
Ngày tải lên: 30/03/2014, 08:20
Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx
... E1, at the bottom of which lies the ThDP cofactor some 2 0–2 5 A ˚ from the protein ÔsurfaceÕ [14]. Thus it may be that these mutations are affecting the efficacy of the recognition of the tethered ... relevant temperature and the catalytic activity at that temperature was then determined. The inactivation temperatures for all the E1 mutants were found to be 5–1 0 °...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Noninvasive imaging of microRNA124a-mediated repression of the chromosome 14 ORF 24 gene during neurogenesis potx
... G2-4d G2-6d + ++ + – – – MAP2 NeuroD Oct4 β-actin c14orf24 β-actin 4 days 2 days 4 days 2 days 4 days Day after non treatment Day after RA treatment ++ + – – – –+ + 70 60 50 40 30 20 10 0 ++ – + Day ... showed that c14orf24 proteins were predominantly present in the cytoplasm of P19 cells before the treatments, and that they disappeared during the neuronal diffe...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Site-directed mutagenesis of selected residues at the active site of aryl-alcohol oxidase, an H2O2-producing ligninolytic enzyme pot
... that this residue could affect the FAD environment, modulating the catalytic rate of the enzyme. Finaly, L315 affects the enzyme k cat , although it is not located in the near vicinity of the ... are situated at similar distances from the hydroxyl of the docked substrate, they could cooperate in facilitating the hydride transfer from sub- strate to FAD. The decrease of a...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a hemeregulated phosphodiesterase from Escherichia coli pptx
... study M95A – – – 0.0013 530 [5] M95L – – – 0.0017 410 [5] M95H – – – 0.016 43 [5] D40A – – – 0.051 14 [13] D40N – – – 0.033 21 [13] a Fast O 2 dissociation (or fast auto-oxidation) may have hampered the ... values. The Fe(II)–O 2 complexes and auto-oxidation We attempted to obtain spectra of the Fe(II)–O 2 heme complexes of the Ec...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: "Automatic Detection of Poor Speech Recognition at the Dialogue Level" potx
... features) suggest that there is some redundancy between these feature sets (at least with respect to the task). Fourth, the fact that the best estimated accuracy was achieved using all of the ... 3: Feature representation of dialogue D1. The dialogue quality features attempt to capture aspects of the naturalness of the dialogue. Rejec- tions represents the number of...
Ngày tải lên: 23/03/2014, 19:20