Báo cáo khoa học: Site-directed mutagenesis, kinetic and inhibition studies of aspartate ammonia lyase from Bacillus sp YM55-1 pptx
Báo cáo khoa học: Site-directed mutagenesis, kinetic and inhibition studies of aspartate ammonia lyase from Bacillus sp. YM55-1 pptx
... estimate obtained from grafit software. Kinetics of reversible inhibition of AspB, T141A and H188A by 3-nitropropionate and D-malate The reversible inhibition of AspB and the T141A and H188A mutants ... from Bacillus sp. YM55-1 FEBS Journal 276 (2009) 2994–3007 ª 2009 The Authors Journal compilation ª 2009 FEBS 3007 Site-directed mutagenesis, kinetic and in...
Ngày tải lên: 29/03/2014, 23:20
Báo cáo khoa học: Crystal structure determination and inhibition studies of a novel xylanase and a-amylase inhibitor protein (XAIP) from Scadoxus multiflorus pot
... increasing concentrations of XAIP (A) and of a-amylase from Bacillus licheniformis with increasing concentrations of XAIP (B). S. Kumar et al. Crystal structure and inhibition studies of XAIP FEBS Journal ... internal space of the TIM barrel considerably. Of the three observed cis peptides, two (Gly33–Phe34 and Trp253–Asp254) are found at the ends of b-strands b2...
Ngày tải lên: 29/03/2014, 09:20
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... the structures of PSs from E. coli and M. tuberculosis. Structural analyses Analysis of the sites of substrate binding from NMR data, and structural comparison of the E. coli, M. tuberculosis and A. ... Enzymatic and other biochemical studies have shown that for PS from M. tuberculosis, pantoate, ATP and b-alanine have K m values of 0.13 mm, 2.6 mm and 0.8 mm, re...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... 38-kDa spe- cies and Ser-Asp-Met-Glu-Lys- for the 19-kDa species, strongly suggesting that respective protein bands Fig. 1. Schematic representation of the structure of the precursor of kumamolisin-As, ... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensities o...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học "ADIABATIC TEMPERATURE RISE AND REACTION RATE OF MASS STRUCTURE IN LOTTE CENTER HANOI PROJECT " pptx
... layer of vinyl + 3 layers of curing blanket + roof 20 days (a) Temperature analysis of the total cross section of mat foundation (b) Stress dispersion of the total cross section of ... during and after curing (c) TCI of the total cross section of mat foundation during and after curing Figure 5. Full-Scale thermal stress and TCI of mat foundation 4....
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa pptx
... matching parts of the sense and antisense strands of the DNA sequence were used. The synthesized fragment was inserted between the BamHI and XhoI sites of pGEX-6P-1. Nucleotide sequences of the recombinant ... replicate experiments. 21 0 119 90 65 4 9 3 7 2 9 ( kDa ) 21 9 S I S ISISI S I S I 4 AaCter TpN15 TpN17 T pN47 4 A aCt e r - TpN15 ATG 4 A aCte r TpN15 T pN17 TpN47 4 Aa C t...
Ngày tải lên: 29/03/2014, 09:20
Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 docx
... this question. Seven PLAs and five 3FTx were purified from the KBf venoms, and respective cDNAs were cloned from venom glands of one of the snakes. Comparison of their mass and N-terminal sequence revealed ... 525 Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic anal...
Ngày tải lên: 16/03/2014, 12:20
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... 85% inhibition of BHLmL2 and BHAb, respectively, while 1 mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BH enzymes ... modification of Asp, Glu, Cys and Ser are shown in Table 5. No significant inactivation of BHs from either L. maculans or A. brassicicola was observed upon treatment wi...
Ngày tải lên: 18/02/2014, 14:20
Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf
... on splicing. A variety of tools are available to us for this purpose and further investigation of these sequence variants will not only further our understanding of the splicing mechanism and ... and further elucidation of this complex and interesting system. Evolutionary studies represent another important field for the investigation of the ele- ments involved in splicing...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... those from a mouse antibody to avoid immunogenicity of the antibody framework from a different species [23–25]. The success of the series of studies shows that the stable framework structure of Fv ... 38] Y/S library in all the CDRs of heavy chain and CDR3 of light chain [ref 39] 10 FN3 Randomization in CDR loops of heavy and light chains Randomization in BC, DE and...
Ngày tải lên: 06/03/2014, 11:20