Báo cáo khoa học: Site-directed mutagenesis, kinetic and inhibition studies of aspartate ammonia lyase from Bacillus sp YM55-1 pptx
Báo cáo khoa học: Site-directed mutagenesis, kinetic and inhibition studies of aspartate ammonia lyase from Bacillus sp. YM55-1 pptx
... estimate obtained from grafit software.Kinetics of reversible inhibition of AspB, T141A and H188A by 3-nitropropionate and D-malateThe reversible inhibition of AspB and the T141A and H188A mutants ... from Bacillus sp. YM55-1 FEBS Journal 276 (2009) 2994–3007 ª 2009 The Authors Journal compilation ª 2009 FEBS 3007 Site-directed mutagenesis, kinetic and inhibition studies of aspartate ammonia lyase ... (1997) The structure of L -aspartate ammonia- lyase from Escherichia coli. Biochemistry 36,9136–9144.13 Karsten WE, Gates RB & Viola RE (1986) Kinetic studies of L-aspartase from Escherichia...
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Báo cáo khoa học: Crystal structure determination and inhibition studies of a novel xylanase and a-amylase inhibitor protein (XAIP) from Scadoxus multiflorus pot
... increasing concentrations of XAIP (A) and of a-amylase from Bacillus licheniformis with increasing concentrations of XAIP (B).S. Kumar et al. Crystal structure and inhibition studies of XAIPFEBS Journal ... internal space of the TIM barrelconsiderably. Of the three observed cis peptides, two(Gly33–Phe34 and Trp253–Asp254) are found at theends of b-strands b2 and b8, respectively. These arepart of ... presence of XAIP, the activ-ities of a-amylase from Bacillus licheniformis [18] and xylanase from fungus Penicillium furniculosum [9] of family GH11 were inhibited considerably. The inhibition of...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... thestructures of PSs from E. coli and M. tuberculosis.Structural analysesAnalysis of the sites of substrate binding from NMR data, and structural comparison of the E. coli, M. tuberculosis and A. ... Enzymatic and other biochemical studies have shown that for PS from M. tuberculosis, pantoate, ATP and b-alaninehave Kmvalues of 0.13 mm, 2.6 mm and 0.8 mm,respectively. Enzymatic studies of plant ... thecontribution of this dimerization to the properties of the enzyme is not clear. Kinetic and structural studies of E. coli PS [3,8,13] and M. tuberculosis PS [7,11,12]suggest that active sites of the...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... 38-kDa spe-cies and Ser-Asp-Met-Glu-Lys- for the 19-kDa species,strongly suggesting that respective protein bandsFig. 1. Schematic representation of the structure of the precursor of kumamolisin-As, ... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentageintensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and ... different amounts of the 38-kDa mature form ( s) and the wild-typeenzyme (d) on the rate of ligation. Catalytic and substoichiometric amounts (molar ratio of 0.01 and 0.1, respectively) of these proteins...
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Báo cáo khoa học "ADIABATIC TEMPERATURE RISE AND REACTION RATE OF MASS STRUCTURE IN LOTTE CENTER HANOI PROJECT " pptx
... layer of vinyl + 3 layers of curing blanket + roof 20 days (a) Temperature analysis of the total cross section of mat foundation (b) Stress dispersion of the total cross section of ... during and after curing (c) TCI of the total cross section of mat foundation during and after curing Figure 5. Full-Scale thermal stress and TCI of mat foundation 4. Hydration history of mat ... of the Mock-up Figure 2. 5.7m ⅹ 5.7m ⅹ 5.7m location of hydration heat sensor and image 3. Analysis of internal restraining stress for mat foundation 3.1 The amount of hydration heat and...
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Báo cáo khoa học: Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa pptx
... matching parts of the sense and antisense strands of the DNA sequence were used. Thesynthesized fragment was inserted between the BamHI and XhoI sites of pGEX-6P-1. Nucleotide sequences of the recombinant ... replicateexperiments.2101199065493729(kDa)219SISISISISISI4AaCterTpN15TpN17TpN474AaCter-TpN15ATG4AaCterTpN15TpN17TpN474AaCter-TpN174AaCter-TpN476xHisPP4AaCter4AaCter4AaCter4AaCterpGEX-ΔGSTPtacABFig. 5. Design and expression of 4AaCter–TpNs. (A) Schematic structure of 4AaCter–TpNs. TpN15, 17 and 47 were fused with a 6 · Histag, 4AaCter 696–851 and the recognition site of PreScission ... involvedin the crystallization and stabilization of Cry toxin.The C-terminal extension of Cry4Aa is structurallysimilar to that of Cry1 [17]. Eight of the 13 cysteines of Cry4Aa are located in the...
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Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 docx
... this question. Seven PLAs and five 3FTx were purified from theKBf venoms, and respective cDNAs were cloned from venom glands of one of the snakes. Comparison of their mass and N-terminal sequencerevealed ... 525Sequences, geographic variations and molecularphylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2Inn-Ho Tsai1, ... prey of Bf and king cobra consists mainly of snakes and reptiles,which are distinct from those of other kraits (e.g.B. candidus and B. multicinctus, which prey on smallcatfishes, eels and rodents...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... 85% inhibition of BHLmL2 and BHAb, respectively, while 1 mm 2-mer-captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively.The influence of pH on the activities of the BHenzymes ... modification of Asp, Glu, Cys and Ser are shown in Table 5. Nosignificant inactivation of BHs from either L. maculansor A. brassicicola was observed upon treatment withreagents specific for Asp and Glu ... FEBSSubstrate specificity and inhibition of brassininhydrolases, detoxifying enzymes from the plant pathogensLeptosphaeria maculans and Alternaria brassicicolaM. Soledade C. Pedras, Zoran Minic and Vijay...
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Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf
... on splicing.A variety of tools are available to us for this purpose and further investigation of these sequence variants willnot only further our understanding of the splicingmechanism and ... and further elucidation of this complex and interesting system. Evolutionary studies representanother important field for the investigation of the ele-ments involved in splicing regulation and ... thataffect splicing and diseaseClinical studies identifying aberrant splicing mutationsare of great importance for genetic counselling, as agood proportion of unclassified variants are oftenfound...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... those from a mouse antibody toavoid immunogenicity of the antibody framework from a different species [23–25]. The success of the series of studies shows that the stable framework structure of Fv ... 38]Y/S libraryin all the CDRs of heavy chain and CDR3 of light chain[ref 39]10FN3Randomization in CDR loops of heavy and light chainsRandomizationin BC, DE and FG loopsFvFig. 4. Local ... VEGFwere selected from a restricted library constructed from only Tyr, Ser, Asp, and Ala, and X-ray structuralanalysis demonstrated the importance of Tyr residues[38]. The construction of more restricted...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ