Báo cáo khoa học: Effect of monovalent cations and G-quadruplex structures on the outcome of intramolecular homologous recombination doc

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... affect the association of the substrate with the enzyme in anyone of several ways, e.g. through solvation effects on substrates or enzyme active sites and through theireffects on the thermodynamic ... (a) the main driving force for the folding is the unfavourableinteraction between the osmolyte and the peptide back-bone, and (b) the total contribution of side chains to the stability of the ... have little or no effect on the solvation properties of the substrate and the enzyme active sites or on theirthermodynamic activities. Another explanation forthese observations comes from our...

Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35ﬁTyr mutant pdf

... while the other one extends into the solvent and does not form any hydrogen bond with the proteinatoms. The former propionate displays the conservedconformation in the structures of the Phe35ﬁTyr ... toward the heme. The mutation from the nonpolar residue Phe35 to the polarresidue Tyr35 makes slight changes in the side chainconformation of this residue. The shift of the Ca atom of Tyr35 of the ... 2002conformation of the heme in the Phe35ﬁTyr mutant isbasically the same as that in wild-type cyt b5. One of the twopropionates is hydrogen bonded to the main- and side chainatoms of Ser64,...

Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc

... stabilize the homodimers (datanot shown). These results suggest that the stabilization of CD38 homodimers by crosslinkers depends strongly on the conformation and orientation between the CD38monomers.It ... expression of CD38 on the plasma membrane and the half-lives of these CD38 mutant proteins on the plasma membrane were signiﬁcantly reduced. Together,these results show that CD38 is expressed as noncova-lently ... values of each of the clones). The relative enzyme activity of each of the mutants is given in parentheses. It was determined by setting the Vmaxadjustedactivity of CD38-WT to 100% and then...

Tài liệu Báo cáo khoa học: FGF-2, IL-1b and TGF-b regulate ﬁbroblast expression of S100A8 doc

... For other controls, pri-mary or secondary antibodies were omitted.Acknowledgements The authors are obliged to Professor Rolf Howlett for the preparation and donation of wound specimens and Drs ... upstream of the transcrip-tion initiation site, untranslated intron 1 and sequencesupstream of exon 1, were used to evaluate activities of deletion constructs after transient transfection into ... notinduced under any condition. Induction of S100A8 in the absence of S100A9 was conﬁrmed in primary ﬁbroblasts. S100A8 mRNA induction byFGF-2 and IL-1b was partially dependent on the mitogen-activated-protein-kinase...

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... represents the node, “X” represents the constitute label, the headword or POS of the headword of the node.In the following, the units of PIQ are bits. ConclusionAmong the feature types in the same ... possibility to modify the headword) the exact headword information 3.7333(Y= the headword of the current constitute) Conclusion The predictive information quantity of the headword of the current node ... summation of the feature type combinations6 Conclusion The paper proposes an information-theory-basedfeature type analysis method, which not onlypresents a series of heuristic conclusion on the predictive...

Báo cáo khoa học: "Constituent-Based Morphological Parsing: A New Approach to the Problem of Word-Recognition" pdf

... Reduplication is the verbal reduplication morpheme. Of interest in this example are regressive Vowel Harmony 4, and, of course, reduplication. The input consists of the stream of segments and a ... cliticization. Also exemplary of the division of duty between the morphophonological parser and the syntactic parser is the dual status of subcategorization in Warlpiri. For example, the ergative ... but the fine details of the phonological structure are not needed. The potential non-isomorphism between phonological and syntactic structure is derived from the narrow bandwidth of the channel...

Báo cáo khoa học: Energetic coupling along an allosteric communication channel drives the binding of Jun-Fos heterodimeric transcription factor to DNA docx

... in concert, their effect is much greater than the sum of the individualparts. Indeed, the effect of the double-alanine substitu-tion of basic residues within the Jun-Fos heterodimer on the ... substitu-tion of all other residues reduces the binding of the Jun-Jun homodimer to DNA by more than one order of magnitude, even though alanine substitution of these residues alone in the context of ... various constructs of the Jun-Fos heterodimer were obtained from the ﬁt of a one-site model, based on the binding of a ligand toa macromolecule using the law of mass action, to the corresponding...

Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc

... indicatethat a functional deletion of Synoviolin causes a changein the intracellular trafﬁcking of NCT. Effect of Synoviolin on the production of AbNCT is one of the essential cofactors of the c-secretasecomplex. ... mostlikely that the accumulation of NCT in synoviolin-nullcells is a result of the suppression of the degradation of NCT. To further investigate this, we next com-pared the degradation of NCT with ... B, the overexpression of Synoviolinenhanced the production of Ab40 and Ab42 by abouttwofold, whereas the secretion of soluble APP was notchanged in these cells. Figure 5C also showed that the endogenous...

Báo cáo khoa học: Functional interplay between viral and cellular SR proteins in control of post-transcriptional gene regulation pptx

... binding to a con-sensus element within the long control region of the viral genome, and also functions together with the E1protein in viral DNA replication [16]. The E2 protein consists of the N-terminal ... inter-actions of SR proteins [1–5]. Reversible phosphoryla-tion of SR proteins is important for assembly and function of the spliceosome and for proper regulation of alternative splicing, and also controls ... spectro-scopic characterization of the coronavirus infectiousbronchitis virus nucleoprotein and elucidation of the role of phosphorylation in RNA binding by using sur-face plasmon resonance. J Virol 79...

Báo cáo khoa học: Direct CIII–HﬂB interaction is responsible for the inhibition of the HﬂB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx

... ATP-dependence of proteolysis of r32by HﬂB. (Upper)Band corresponding to r32 on 11% SDS ⁄ PAGE, in the presence orabsence of 5 mM ATP. Numbers on the top of each lane indicate the time of digestion ... thismode of antiproteolytic action of CIII a general mode of CIII activity, or does it apply only for CII? Weexamined the mode of the inhibitory action of CIII on proteolysis of the heat shock ... a function of concentration (Fig. 3). Under the conditions of ourexperiment, near-total inhibition by CIIIC could beobserved, whereas in the presence of CIII, only partialinhibition was achieved....

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