Báo cáo khoa học: Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps docx

... 2009 The Authors Journal compilation ª 2009 FEBS MINIREVIEW Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps Natalia ... of the presence of various ThDP intermediates on the enzymes, and the informa- tion that has emerged regarding the state of ionizat...

Ngày tải lên: 29/03/2014, 23:20

... between HEThDP and FAD is the major source of catalytic stimulation related to this activation phenomenon. The X-ray structure of EcPOX could be solved in the rest- ing and in the activated state and revealed ... depletion of the thiamin radical and reduction of all clusters is only achieved after addition of CoA. What is the special role of CoA for propagati...

Ngày tải lên: 18/02/2014, 08:20

... for acetate and the other for propionate. The remarkable difference is that the overall activity for activation of acetate is always higher in A. nidulans than that for the activation of propionate ... to grow at the expense of this carbon source. The main pathways involved in propionate metabolism are that of the methylmalonyl- CoA pathway and the methylcitrate...

Ngày tải lên: 23/03/2014, 15:20

... different substrates were investigated as described in Materials and methods. The vertical arrow indicates the point of cleavage. One mM of each substrate was used for the determination of relative hydrolytic ... analysis of the reaction mixture obtained by endo-b- galactosidase-mediated transglycosylation and time courses of the pro- duction of transglycosylation p...

Ngày tải lên: 31/03/2014, 07:20

... signaling pathways by the pathogen. Upon infection, the phosphatases and kinases of several pathogenic bacteria modify host proteins and help in the establishment of the disease. The uptake of M. ... better understanding of these mechanisms and the host–path- ogen interaction will be essential not only to control this pandemic, but also to elucidate the novel fea...

Ngày tải lên: 16/02/2014, 15:20

... s under evaluation. Comparative FACS analysis of the internalization of the full-length Tat protein construct and the Tat CPP Differences in the mechanisms of internalization between the Tat peptide ... such treatment abolished the internalization of the GFP-fused Tat protein but did not alter the uptake of the Tat CPP. These biochemical evidences con®rmed a pa...

Ngày tải lên: 21/02/2014, 03:20

... substrates. The interpretation of this research has been further complicated by the observation that many of its pSer ⁄ Thr-Pro substrate motifs are located in natively unstructured regions of polypeptides, ... polypeptides, and are characterized by minor populations of the cis conformer. Finally, we review the data on the possibility of alternative modes of substrate...

Ngày tải lên: 07/03/2014, 05:20

... to avoid limitation by the coupled system, the rates were below 0.2 units at A 340 per min. The rate was calculated assuming that two ADP are generated during the reaction (due to the presence of NDP ... 7.5, 5m M MgCl 2 and the substrates at 37 °C. The reaction was stopped at 2, 4 and 6 min by adding 3 lL aliquots of the reaction mixture to 2 lL stop solut...

Ngày tải lên: 08/03/2014, 02:20

... demonstra- ting that the rate-limiting step belongs to the oxidative half- reaction. The oxidative half -reaction A major finding of this study is that the apparent rate of reoxidation of the free reduced ... glycine and sarcosine and was approximately proportional to the concentration of D -proline. This observation indicates that the rate of flavin reduction is s...

Ngày tải lên: 08/03/2014, 08:20

... subtraction of data from a series of injections of copper chelate solution into a buffer blank correlating to the heat of dilution of the copper complex. After subtraction of the blank data a non- linear ... Inhibition of amyloid fibril growth as observed by TEM. Samples at 34 lM protein concentration were incubated at 25 °C in the stand- ard fibrillation buffer and...

Ngày tải lên: 19/02/2014, 06:20