Báo cáo khoa học: Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps docx
Báo cáo khoa học: Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps docx
... 2009 The Authors Journal compilation ª 2009 FEBSMINIREVIEW Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps Natalia ... of the presence of variousThDP intermediates on the enzymes, and the informa-tion that has emerged regarding the state of ionization and tautomerization of the AP ring on these intermedi-ates. ... for the presence of the conjugate base in the activated or unactivated forms of YPDC [51].It is important to emphasize that determination of the state of ionization and tautomerization of enzyme-bound...
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Tài liệu Báo cáo khoa học: Reaction mechanisms of thiamin diphosphate enzymes: redox reactions pdf
... betweenHEThDP and FAD is the major source of catalyticstimulation related to this activation phenomenon. The X-ray structure of EcPOX could be solved in the rest-ing and in the activated state and revealed ... depletion of the thiamin radical and reduction of all clusters is only achievedafter addition of CoA. What is the special role of CoAfor propagation of the second electron and the associ-ated 105-fold ... understanding of reductive acetylation and active site coupling betweenE1 and E2 [39,40]. The active center of E1 with the ThDP cofactor is located at the bottom of a long fun-nel-shaped substrate...
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Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt
... foracetate and the other for propionate. The remarkabledifference is that the overall activity for activation of acetate is always higher in A. nidulans than that for the activation of propionate ... to grow at the expense of this carbon source. The main pathways involved inpropionate metabolism are that of the methylmalonyl-CoA pathway and the methylcitrate cycle. The reaction of methylmalonyl-CoA ... inoculated on eachplate and incubated for 72 h at 37 °C and photographed.Synthesis of cDNA and sequence analysisPropionate grown cultures of A. fumigatus ATCC46645were used for preparation of...
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Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
... different substrates were investigated as described inMaterials and methods. The vertical arrow indicates the point of cleavage. One mM of each substrate was used for the determination of relative hydrolytic ... analysis of the reaction mixture obtained by endo-b-galactosidase-mediated transglycosylation and time courses of the pro-duction of transglycosylation products 9 and 10 from 1 and degradation of ... a fairly good substratedespite the absence of a Gal group at the terminal position,because the kcat/Kmvalue of 1 was 31% of that of 2.However, the kcat/Kmvalue of 4, in which Glc had...
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Tài liệu Báo cáo khoa học: Survival mechanisms of pathogenic Mycobacterium tuberculosis H37Rv ppt
... signaling pathwaysby the pathogen. Upon infection, the phosphatases and kinases of several pathogenic bacteria modify hostproteins and help in the establishment of the disease. The uptake of M. ... betterunderstanding of these mechanisms and the host–path-ogen interaction will be essential not only to controlthis pandemic, but also to elucidate the novel features of macrophage defenses and host ... (e.g.microarrays and proteomics), in combination withmodern approaches, has facilitated a more rational and directional approach towards the understanding of these mechanisms. Therefore, it is clear that...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... sunder evaluation.Comparative FACS analysis of the internalization of the full-length Tat protein construct and the Tat CPPDifferences in the mechanisms of internalization between the Tat peptide ... such treatmentabolished the internalization of the GFP-fused Tat proteinbut did not alter the uptake of the Tat CPP. Thesebiochemical evidences con®rmed a pathway for the e ntry of the Tat peptide ... routes for the Tat CPP and the Tat protein.What might be the reasons underlying the observeddifferences in cellular uptake between the Tat CPP and the GST±Tat±GFP protein, that both contain the same...
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Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx
... substrates. The interpretation of this research has been further complicated by the observation that many of its pSer ⁄ Thr-Pro substrate motifs are located innatively unstructured regions of polypeptides, ... polypeptides, and are characterized byminor populations of the cis conformer. Finally, we review the data on the possibility of alternative modes of substrate binding and the complex rolethat Pin1 ... isomerization,therefore await further structural elucidation.Structural features of Pin1 substratesConcerning the function of Pin1, a first intriguingobservation is that many of its substrates...
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Báo cáo khoa học: Reaction of human UMP-CMP kinase with natural and analog substrates docx
... toavoid limitation by the coupled system, the rates were below0.2 units at A340per min. The rate was calculated assumingthat two ADP are generated during the reaction (due to the presence of NDP ... 7.5,5mMMgCl2 and the substrates at 37 °C. The reaction was stopped at 2, 4 and 6 min by adding 3 lL aliquots of the reaction mixture to 2 lL stop solution consisting of 1 mMformic acid and 10 mMeach of ... [16]. AMP kinase catalyses the reversible ATP-dependent synthesis of ADP from AMP. Therefore the reverse reaction forms ATP and AMP from two ADPmolecules. We ask the question whether UMP-CMP kinasealso...
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Báo cáo khoa học: Kinetic mechanisms of glycine oxidase fromBacillus subtilis docx
... demonstra-ting that the rate-limiting step belongs to the oxidative half- reaction. The oxidative half -reaction A major finding of this study is that the apparent rate of reoxidation of the free reduced ... glycine and sarcosine and was approximately proportional to the concentration of D-proline. This observation indicates that the rate of flavin reduction is still quite close to that of reoxidation of ... reduction with the individual substrates and its reductive half -reaction was demonstratedto be reversible. The rates of flavin reduction extrapolated tosaturating substrate concentration, and under...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... subtraction of data from a series of injections of copper chelate solutioninto a buffer blank correlating to the heat of dilution of the copper complex. After subtraction of the blank data a non-linear ... Inhibition of amyloid fibril growth as observed by TEM. Samples at 34 lM protein concentration were incubated at 25 °C in the stand-ard fibrillation buffer and after 9000 min (the plateau level of the reaction) ... in terms of the heat changeper mole of ligand against the ligand to macromoleculeratio. From the binding isotherm values for the reaction stoichiometry, association constants Ka, the change...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát hiện xâm nhập dựa trên thuật toán k meansĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam