Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot

... FEBS in vitro model in order to investigate some of the fea- tures of the polyamine–phosphate interactions. Specifi- cally, we examined the role played by each polyamine in the self-assembly of in ... named nuclear aggregates of polyamines (NAPs). Interestingly, other authors have described the phosphate-induced self-assembly of polyamines in a different...

Ngày tải lên: 29/03/2014, 23:20

... responsive cells. These data provide a possible model of drug interaction in colorectal cell lines, which involves the complex interplay of the molecules regulating the cell cycle. Clinically, the cytostatic ... also reduced in the plates Fig. 3. Effect of combining CDO with cyto- toxic agents in HCT116 and GEO cells. The numerical sum of BrdU incorporation into cultu...

Ngày tải lên: 07/03/2014, 15:20

... con- version of GDP-Man into products in the presence of both enzymes (e.g. with Drosophila GMD alone the conversion of GDP-Man into the intermediate was  80%, whereas in the presence of both enzymes the conversion ... (alternatively spliced form of GMD-1), gmd-2 and ger-1. The amounts of cDNA used in the PCRs were normalized on the basis of the intensit...

Ngày tải lên: 16/03/2014, 13:20

... members of the nuclear receptor superfamily [5] and exhibit a typ- ical modular structure comprising the N-terminal A ⁄ B domain, the DNA-binding or C domain, the hinge or D domain, the ligand-binding ... responsible for the increased activity of LmRXR. Comparison of amino acid sequences present in the ligand-binding domains of HsRXR and LmRXR showed that most of...

Ngày tải lên: 07/03/2014, 12:20

... simulation of DK9 thrombin in explicit water solvent supports the role of the A-chain in affecting the conformation and catalytic properties of the B-chain, particularly in some insertion loops of the ... flexibility of their side-chains to the solvent the smaller the intrinsic fluorescence of the enzyme. The smaller gain in the intrinsic fluorescence o...

Ngày tải lên: 07/03/2014, 12:20

... for the inhibi- tion of the protein expression by CSP using two models. In the most simple model the inhibition is predominantly due to the binding of CSP to one component of the system (e.g. a ... function of CSPs, and for potentially improving the performance of the combined in vitro transcription ⁄ translation system, we investigated the in uence of dif...

Ngày tải lên: 07/03/2014, 21:20

... upon cysteine exchange are the black appearance of the C30S protein and the orange colour of the C130S mutant. Changing the other cysteine within these pairs did not result in matching colour ... bridge and the introduction of a polar group probably interferes with binding of the adenine portion of the FAD that is normally attached to this region of the protein...

Ngày tải lên: 17/03/2014, 10:20

... peptide–membrane interaction. The magnitude of the membrane dipole potential is affected by membrane binding and by the insertion of molecules (including peptides). The change of the potential mag- nitude ... partition into membranes is present in the short segment at the end of the sequence, corresponding to the cholesterol- binding domain (MPRP-S). The peptides...

Ngày tải lên: 23/03/2014, 07:20

... the dynamics of the network in terms of a set of nonlinear ordinary differential equations. Mass-flow description of the UTase subnetwork and binding of a-KG to PII Any of the three subunits of ... value of 20% (for 1.0 mm a-KG) for ammonium in excess of 1 mm (data not shown). The heights of both the peak in the dependence of the variation of the...

Ngày tải lên: 23/03/2014, 13:20

... Jun N-terminal kinases (JNKs) and mitogen activated protein kinases. JNKs phosphorylate the N terminus of the trans- acting domain of Jun, thereby increasing its transactiva- tion potency [13–16]. Inhibition ... All of the following steps were performed at 4 °C. The membrane strip containing the sample was cut and incubated in denaturing solution (6 M guanidine/HCl in 1 ·...

Ngày tải lên: 23/03/2014, 20:22