Báo cáo khoa học: Accumulation of polyubiquitinated proteins by overexpression of RBCC protein interacting with docx

... 6385 Accumulation of polyubiquitinated proteins by overexpression of RBCC protein interacting with protein kinase C2, a splice variant of ubiquitin ligase RBCC protein interacting with protein ... shuttling protein RBCC protein interacting with protein kinase C1 (RBCK1) possesses transcriptional and ubiquitin ligase activities. We have recently...

Ngày tải lên: 29/03/2014, 22:21

... MINIREVIEW 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein ligand complexes Kiyoshi Ozawa, Peter ... synthesis of proteins in the presence of other pro- teins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into ......

Ngày tải lên: 07/03/2014, 12:20

... helicase clearly interacts with the C-terminus of NS3 (Fig. 1B) [7]. By structural modelling of an inhibitor binding to full-length NS3, we found that most of the contacts were with the pep- tide backbone, ... the lower yields and stabilities com- pared with those obtained with the truncated protease, it is the protein that has been used in all of our studies of the NS3...

Ngày tải lên: 16/03/2014, 06:20

... PTN colocalizes with that of surface-expressed nucleolin. Results Inhibition of HIV-infection by PTN We investigated the inhibitory effect of PTN on HIV infection by using the experimental model of HeLa ... synthesized by MWG (Ebersberg, Germany). The presence of the muta- tions was confirmed by double stranded DNA sequencing. Transfections of CHO-K1 cells with recombin...

Ngày tải lên: 30/03/2014, 20:20

... shown). M proteins mediate the binding of GAGs to streptococci To establish the role of M proteins for the GAG interaction we again first focused on the AP1 system. AP1 expresses two members of the M protein ... findings with purified proteins, recom- binant M1 protein and protein H were applied in slots to a nitrocellulose membrane and probed with 125 I-labelled DS. As a co...

Ngày tải lên: 20/02/2014, 11:20

... acquisition, the idea of virtual accumulation, abstraction levels on LSs, and a design for organization and exploitation of large lexical systems based on the notions of accumulation. 1 Acquisition ... function model of CLOS). Finally, the values of the output variables of the left-hand side nonterminal (aol, ao2 ) are collected and returned as the result of its expand...

Ngày tải lên: 20/02/2014, 18:20

... flavoproteins [17]. The correlations found now allows us to determine, with some confidence, the endocyclic angle / of the N(5) and the N(10) atom of reduced flavoproteins by the method developed by ... the electronic structure of the protein- bound cofactor and its interactions with the apoproteins. Strong hydrogen bonds exist between protein- bound flavin and the apoproteins at...

Ngày tải lên: 16/03/2014, 16:20

... (B) The numbers of predicted flavoproteins as percentages of the total proteins for the species in (A). (C) The RIs of flavoproteins in these genomes. Yellow bars indicate genomes with low redundancy, ... FMN-utilizing proteins and  1160 for FAD-utilizing proteins) . Analysis of the structural classification for FMN-dependent proteins reveals a strong bias towards the clan Nitr...

Ngày tải lên: 28/03/2014, 22:20

... (B) The numbers of predicted flavoproteins as percentages of the total proteins for the species in (A). (C) The RIs of flavoproteins in these genomes. Yellow bars indicate genomes with low redundancy, ... FMN-utilizing proteins and  1160 for FAD-utilizing proteins) . Analysis of the structural classification for FMN-dependent proteins reveals a strong bias towards the clan Nitr...

Ngày tải lên: 28/03/2014, 22:21

... protein N-myristoyla- tion in plants. We first evaluated the utility of this system for analysis of N-myristoylated proteins. N-myristoylation of the tested proteins was detected by labeling with ... of in vitro-synthesized N-myristoylated proteins To confirm the N-myristoylation of proteins synthe- sized with the cell-free system, we next performed MALDI-TOF MS analys...

Ngày tải lên: 29/03/2014, 21:20