Báo cáo khoa học: Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin potx

... mutant toxins and the analysis of their binding activity to the receptor to investigate the role of mutated amino acid residues; (b) the analysis of the influence of the modification of the cysteine ... comparison with DF, the inhibitory effect of IAF should be restricted to a narrower region. The region on Cry1Aa toxins for contact and interac...

Ngày tải lên: 29/03/2014, 22:21

... subtracted from the consumption recorded after addition of 1 m M phenol. The effects of PHI and PHR concentrations on the overall PH activity were evaluated by systematic variation of PHI concentration (0.3; ... shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reflects the occurr...

Ngày tải lên: 21/02/2014, 00:20

... a soft ligand and therefore shows a preference for soft metals. More- over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ion. ... to air resulted in the slow formation of a disulfide linkage by cysteine oxidation of the a-domain and a concominant release of one Zn ion, but the spec- troscopic feat...

Ngày tải lên: 16/02/2014, 15:20

... to stabilization of the sol state of silica. One mechanism for the gel to sol conversion could be hydrolysis of the oxygen bridge of the poly- merized ⁄ polycondensed silicic acid. The linkage ... bonding between the imidazole nitrogen of the conserved His and the hydroxyl of the active-site Ser increases the nucleophilicity of the Ser oxygen, facilitat...

Ngày tải lên: 18/02/2014, 16:20

... between the subunits in the interface region create conformational changes in both chains. The molecular recognition by the ion channel binding region of the Kunitz module differs from that of other ... other serine proteinases, the loss of proteinase activity at high pH probably results from the deprotonation of the N-terminus and the disruption of the s...

Ngày tải lên: 14/03/2014, 22:20

... of multimerization promoted by the N terminus and the coiled-coil domain [16,17]. However, the consequences of multimerization and association with other proteins for in vivo activity regulation of DMPK ... clear. Activation of Citron kinase and ROCK-I is mediated by RhoA binding to a Rho -binding domain located in the C-terminal part of the coiled-coil region...

Ngày tải lên: 16/03/2014, 13:20

... if L -isoleucine and L -valine are degraded in A. nidulans as they are in yeast, then the formation and conversion of the Fig. 2. D -Galacturonic acid provokes induction of the alc genes. (A) Northern blot analysis ... loss -of- function mutants. In one of the crossfed strains, alc500 TalcR, proper inducibility of alcR and aldA was demonstrated by Northern blot analys...

Ngày tải lên: 17/03/2014, 10:20

... ques- tion 1 through S1. We call contextual information the context of a question in this paper. A summary of forum threads in the form of question-context-answer can not only highlight the main content, ... predicted rums), and then use them to expand question and compute cosine similarity. The structural features of forums provide strong clues for contexts. For examp...

Ngày tải lên: 23/03/2014, 17:20

... regulates the expression level of the P2X 7 receptor. Results Presence of the P2X 7 receptor and its modification in alveolar epithelial cell lines First, we tested the expression of the P2X 7 receptor ... result for P2X 4 in cardiac myocytes. They demonstrated that the gly- cosylation of the P2X 4 receptor is required for its localization to the cytoplasm...

Ngày tải lên: 19/02/2014, 00:20

... easy-to-use and fast. A new system can be built using only a few commands. To control the system, users only need to modify a configuration file. In addition to the special attention to usability, the ... types), including Monotone (M), Swap (S), and Discontinuous (D). 20 probabilities of the three orientations (Galley and Manning, 2008). 3.2 Translation Rule Extraction...

Ngày tải lên: 19/02/2014, 20:20