Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot

... preferential binding of human topoisomerase I to supercoiled DNA is unknown but, if node recognition is important, then it is likely that the binding involves an interaction with two regions of DNA ... subdomain III. The results obtained implicate the linker and solvent-exposed basic residues in core subdomain III in the preferential binding of human t...

Ngày tải lên: 29/03/2014, 22:21

... Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition Troels Wind*, ... incubation for 40 min. The specific inhibitory activity of PAI-1 at the various time- points, i. e. the fraction of the total amount of PAI-1 forming a stable complex...

Ngày tải lên: 20/02/2014, 11:20

... sensitivity of the enzyme to inhibition by agmatine and putrescine, the decarboxylated deriva- tives of arginine and ornithine, respectively (Fig. 1). However, the most important difference was in ... this basis, direct repulsion of the negatively charged Asp130 would explain the increased K m value for arginine inhibition and K i value for ornithine inhibition of the...

Ngày tải lên: 16/03/2014, 12:20

... ÔHis-motifÕ,thatwas suspected to participate in the catalysis. This hypothesis was supported by the ®nding that a single mutation of the Fig. 1. Genes and domain organizations of gramicidin S and tyrocidine A synthetases. These ... to detect a conserved serine residue that might act in the catalytic center of C-domains. To further rule out the possibility of a catalyti...

Ngày tải lên: 17/03/2014, 11:20

... B2). Isolation of mitochondria and measurement of [Mg 2+ ] m by spectrofluorometry The isolation of mitochondria by differential centrifugation and the ratiometric determination of intramitochondrial Mg 2+ concentrations ... shown to mediate Mg 2+ influx into mitochondria. Overexpres- sion of the protein was found to increase Mg 2+ influx into isolated mitochondria, while del...

Ngày tải lên: 23/03/2014, 10:21

... promises to yield significant insight into biological mechanisms. In this section I would like to point out analytical aspects and potentials specific to the analysis at the subcellular level. Microsomal ... phagocytic internalization of foreign material by macrophages. In this analysis, in addition to the description of the phagosome proteome, the maturation of th...

Ngày tải lên: 23/03/2014, 20:22

... catalytic triad or a catalytic diad consisting of only Ser and His is required for catalysis. In this study, we describe a mutational analysis and mechanistic investigations of the microbial TEII Srf (242 amino ... intro- duced in these strictly conserved positions (C18A, S112A Fig. 1. Alignment of 16 TEIIs. TEII srf was aligned together with 14 other TEIIs of microbial secondar...

Ngày tải lên: 30/03/2014, 13:20

... Xiao X (2009) ATP -binding domain of heat shock protein 70 is essen- tial for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis ... further assayed by flow cytometry with annexin V–FITC ⁄ pyridine iodination (PI) double staining, which identified apop- totic cells as annexin V positive, but PI negative. This method...

Ngày tải lên: 16/02/2014, 09:20

... result- ing in hydrophobic interaction between the N-domain and the regulatory TnC -binding site [16]. This inter- action induces the dissociation of the inhibitory region, which is adjacent to the ... nor inhibition. To determine whether the inhibitory effect correlates with the binding affinity to actin-tropomyosin, we examined each TnI for its ability to cosedimen...

Ngày tải lên: 20/02/2014, 01:20

... example, it is one thing to ask for the birthplace of a person, but it is quite another to ask for all locations visited by a person over a specific period of time. Queries may contain several types of ... determining the entities in an event descrip- tion, we rely on lower-level binary classifiers, each of which has been trained to match a specific type 1 Other binnings did no...

Ngày tải lên: 20/02/2014, 09:20