Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot
Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot
... preferential binding of
human topoisomerase I to supercoiled DNA is
unknown but, if node recognition is important, then it
is likely that the binding involves an interaction with
two regions of DNA ... subdomain III. The results obtained implicate the linker and
solvent-exposed basic residues in core subdomain III in the preferential
binding of human t...
Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... Mutational analysis of plasminogen activator inhibitor-1
Interactions of a-helix F and its neighbouring structural elements regulates
the activity and the rate of latency transition
Troels Wind*, ... incubation for 40 min.
The specific inhibitory activity of PAI-1 at the various time-
points, i. e. the fraction of the total amount of PAI-1 forming
a stable complex...
Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... sensitivity of the enzyme to inhibition by
agmatine and putrescine, the decarboxylated deriva-
tives of arginine and ornithine, respectively (Fig. 1).
However, the most important difference was in ... this basis,
direct repulsion of the negatively charged Asp130
would explain the increased K
m
value for arginine
inhibition and K
i
value for ornithine inhibition of the...
Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
... ÔHis-motifÕ,thatwas
suspected to participate in the catalysis. This hypothesis was
supported by the ®nding that a single mutation of the
Fig. 1. Genes and domain organizations of
gramicidin S and tyrocidine A synthetases.
These ... to detect a conserved
serine residue that might act in the catalytic center of
C-domains. To further rule out the possibility of a catalyti...
Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt
... B2).
Isolation of mitochondria and measurement of
[Mg
2+
]
m
by spectrofluorometry
The isolation of mitochondria by differential centrifugation
and the ratiometric determination of intramitochondrial
Mg
2+
concentrations ... shown to
mediate Mg
2+
influx into mitochondria. Overexpres-
sion of the protein was found to increase Mg
2+
influx
into isolated mitochondria, while del...
Báo cáo khoa học: Proteome analysis at the level of subcellular structures Mathias Dreger pot
... promises to yield significant insight
into biological mechanisms. In this section I would like to
point out analytical aspects and potentials specific to the
analysis at the subcellular level.
Microsomal ... phagocytic internalization of foreign material
by macrophages. In this analysis, in addition to the
description of the phagosome proteome, the maturation
of th...
Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis pdf
... catalytic triad or a catalytic diad
consisting of only Ser and His is required for catalysis.
In this study, we describe a mutational analysis and
mechanistic investigations of the microbial TEII
Srf
(242
amino ... intro-
duced in these strictly conserved positions (C18A, S112A
Fig. 1. Alignment of 16 TEIIs. TEII
srf
was aligned together with 14 other TEIIs of microbial secondar...
Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx
... Xiao X (2009)
ATP -binding domain of heat shock protein 70 is essen-
tial for its effects on the inhibition of the release of the
second mitochondria-derived activator of caspase and
apoptosis ... further assayed
by flow cytometry with annexin V–FITC ⁄ pyridine
iodination (PI) double staining, which identified apop-
totic cells as annexin V positive, but PI negative. This
method...
Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... result-
ing in hydrophobic interaction between the N-domain
and the regulatory TnC -binding site [16]. This inter-
action induces the dissociation of the inhibitory region,
which is adjacent to the ... nor
inhibition.
To determine whether the inhibitory effect correlates
with the binding affinity to actin-tropomyosin, we
examined each TnI for its ability to cosedimen...
Tài liệu Báo cáo khoa học: "Event Matching Using the Transitive Closure of Dependency Relations" pdf
... example, it is one thing to ask
for the birthplace of a person, but it is quite another
to ask for all locations visited by a person over a
specific period of time.
Queries may contain several types of ... determining the entities in an event descrip-
tion, we rely on lower-level binary classifiers, each
of which has been trained to match a specific type
1
Other binnings did no...