Báo cáo khoa học: Copines-1, -2, -3, -6 and -7 show different calcium-dependent intracellular membrane translocation and targeting pdf
Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx
... toxic xenobiotics and byproducts of oxidative metabolism. In addition to their catalytic activities, plant GSTs are also involved in the response to different biotic and abiotic stresses, and can be ... aliqu ots were withdrawn and assa yed f or ac tivity. 3504 G. A. Kotzia and N. E. Labrou (Eur. J. Biochem. 271) Ó FEBS 2004 Expression and purification of maize GST I and other...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7 pot
... caspa- ses-1, -2, -3, -4, -6, -7, -11 and CED-3 could all cleave caspase-2, to varying extents [36,38–40]. In contrast, our data indicates that concentrations of caspases-3, -7 and -8 capable ... [44]) and pCUP1-(LEU2)-caspase-7 [35] and ligated into pGALL-(LEU2) to generate pGALL-(LEU2)- caspase-3, pGALL-(LEU2)-caspase-6 and pGALL-(LEU2)- caspase-7, respectively....
Ngày tải lên: 23/03/2014, 13:20
Tài liệu Báo cáo khoa học: Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity docx
... activity and p38 MAPK activation) upstream of GLUT4 translocation and glucose uptake in 3T3-L1 adipocytes. Acknowledgements We thank Drs Hans Joost and Annette Schu ¨ rmann (Aachen and Potsdam, ... These data show that in 3T3-L1 adipocytes both arsenite- and insulin- induced signalling pathways project towards a similar cel- lular response, namely GLUT1 and GLUT4 translocatio...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt
... in noninfected larvae in both anterior and posterior spiracles, in the digestive tract, the ring gland, the antenna buds and testis (Fig. 3A,B and data not shown). In adult flies, GFP was expressed ... expression of both phk-2 and phk-3 is strongly reduced by ecdysone in vivo [41]. These data support the hypothesis that Phk-2 and Phk-3 may interact with ligands different from Phk-1...
Ngày tải lên: 08/03/2014, 08:20
Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... sig- nalling [3–5] and of cytokines such as interleukin (IL )-6 [6]. It is also involved in transformation and tumour progression [7] and its activation, as detected in breast, head and neck, lung and colon ... control ODN (con) and the dead cells were counted after 16, 24 and 48 h of culture using trypan blue staining. (C) Cells were treated with 0.5, 1 and 2 lg of hpST3dO...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... Substrate S-2288. v max and K m values for G372A-FVIIa were 2.2 mODÆmin )1 ÆnM )1 and 7.1 mM and those for for FVIIa were 4.9 mODÆ min )1 ÆnM )1 and 1.8 mM; (B) Substrate S-2366. v max and K m values for ... mODÆmin )1 ÆnM )1 and 3.9 mM and those for FVIIa were 6.9 mODÆmin )1 ÆnM )1 and 1.9 mM; (C) Substrate S-2765. v max and K m values were not estimated; (D) Substrate S-22...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... The pH was adjusted to 2.5, 8.0 and 11.5 and the samples were incubated at 37 °C, 50 °Cand 70 °C. Aliquots were taken at different time-points from each sample and analyzed by MALDI-TOF MS. The addition ... of Met1, and Met23 substituted for Leu, Fig. 3. Comparison of the cyclization reaction as a function of tem- perature and pH. (A), (B) and (C): (d), (s )and( .) correspond to...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: FGF-2, IL-1b and TGF-b regulate fibroblast expression of S100A8 doc
... (Fig. 3D) and cells stimulated for 24 h with IL-1b (samples 8 and 9), FGF-2 (sample 10), or FGF-2 and heparin (samples 4 and 5) did not express the gene. IL-1b and FGF-2 combined (samples 6 and 7, Fig. ... (FGF-2) and transform- ing growth factor-b (TGF-b) regulate fibroblast function, differentiation and proliferation. S100A8 and S100A9 are members of the S100 family of Ca 2...
Ngày tải lên: 19/02/2014, 18:20
Báo cáo khoa học: Typical 2-Cys peroxiredoxins – modulation by covalent transformations and noncovalent interactions doc
... 1 and 3), whereas the remaining single dehydration (reaction 2) brings about the one-electron reduction and oxidation of Cys P and Cys R , respectively. In essence, 2-Cys Prxs employ three different ... whose special photosynthetic trait relies on the differential functioning of chloroplasts in two different types of leaf cell: bundle sheath and mesophyll cells [103]. Notably, 2-C...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Typical 2-Cys peroxiredoxins – structures, mechanisms and functions ppt
... both the FF and LU images, the four residues conserved in all Prxs (C P , Arg, Thr and Pro) and C R are colored green, with sulfur, oxygen and nitrogen atoms colored dark yellow, red and blue, ... in our understanding of the catalytic and regulatory mechanisms of ‘typical 2-Cys’ peroxiredoxins and of the biological roles played by these important enzymes in oxidative stress and...
Ngày tải lên: 07/03/2014, 01:20