Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx

... al. RNA degradosome complex in Vibrio angustum S14 FEBS Journal 277 (2010) 5161–5173 ª 2010 The Authors Journal compilation ª 2010 FEBS 5173 Analysis of the RNA degradosome complex in Vibrio angustum ... its role in mRNA degradation, the degradosome is also responsible for the processing of 5S ribosomal RNA and tRNAs, as well as the degradation...

Ngày tải lên: 29/03/2014, 21:20

... mesoderm 0 min 1 min 2 min 3 min 4 min 9 min B A Fig. 2. Time-lapse LSM analysis of Olvas–GFP reveals the dynamic nature of the nuage. Schematic diagram of the preparation of PGCs of medaka specimen ... diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudor coding sequences were joined to the C-te...

Ngày tải lên: 18/02/2014, 16:20

... by affecting the RNA- binding ability of the resulting heteroheptameric Lsm complex (see Lsm8DC, Lsm818, Lsm881 and Lsm188). Minimal U6 binding by GFP GFP IP Input Lsm7-Myc U6 RNA U4 RNA U4 RNA U6 RNA scR1 GFP-LSM8 GFP-lsm8 ... mutational analysis of Lsm1p showing the importance of residues proposed to be involved in RNA binding and complex formation, and of...

Ngày tải lên: 07/03/2014, 02:20

... [29]. The overall analysis, examining the topology of the phylogenetic tree and the position of each gene inside the cluster, was informative regard- ing the orthology relationships of the distinct ... dashes. The active site (YTVAK) and the cofactor-binding motifs are marked in bold. The D43, T67 and A69 residues, involved in cofactor specificity, are indicated...

Ngày tải lên: 07/03/2014, 09:20

... define further the features in the C-ter- minus of 4E-BP1 that are involved in its binding to raptor. The data for the other truncation mutants shown in Fig. 1C indicate that other regions of 4E-BP1 ... after treatment of cells with insulin [25]. Replacing its N-terminus with the N-terminal part of 4E-BP1 led to a marked increase in its phosphoryla- tion (even in t...

Ngày tải lên: 16/03/2014, 06:20

... the maturation of the enzymes. Most o f them a ct pleiotropically in the synthesis of all four hydrogenases, in particular in the synthesis and insertion of the metal centre [6,7]. Two of the products of the ... transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of the HypE protein yielding a...

Ngày tải lên: 16/03/2014, 18:20

... confirm the presence of the RIP/lectin coding sequence in the tobacco genome. Only those plants that were positive in the PCR analysis were withheld for further analysis. Expression of the RIP/lectin ... Therefore, the presence of mRNAs encoding PR-1, PR-2, PR-3 and proteinase inhibitor II was verified by Northern blot analysis. In none of the transgenic plants c...

Ngày tải lên: 23/03/2014, 12:20

... accommodated within the hydrophobic core of the CUB domain. This core is conserved in the X-ray structures of proteins con- taining the CUB signature, including the mannan- binding lectin-associated ... [11,21,22]. These binding sites are nonetheless cryptic in the heterodimer, which is typically isolated from the nonheparin-binding fraction of boar seminal plasma [11]...

Ngày tải lên: 23/03/2014, 15:21

... different protein variants, we have investi- gated the role of both pro-sequences in the in vitro refolding of PCI, together with studies of the in uence of the N-terminal pro-region on its in vivo ... extremely inefficient folding of PCI in vitro [18], together with the presence of the above-mentioned long pro-sequences, suggest a possible involvement of them...

Ngày tải lên: 23/03/2014, 21:20

... (a) the con- struction of cysteine-substituted mutant toxins and the analysis of their binding activity to the receptor to investigate the role of mutated amino acid residues; (b) the analysis of ... Mapping of the mutation site. Red indicates the amino acid residues that showed inhibition of BtR175 binding by DF binding. Yellow indicates the amino acid residue...

Ngày tải lên: 29/03/2014, 22:21