Báo cáo khoa học: Structural requirements for Caenorhabditis elegans DcpS substrates based on fluorescence and HPLC enzyme kinetic studies pdf

... The Authors Journal compilation ª 2010 FEBS 3013 Structural requirements for Caenorhabditis elegans DcpS substrates based on fluorescence and HPLC enzyme kinetic studies Anna Wypijewska 1 , Elzbieta ... the substrate affinity and kinetics of this reaction with the substrates were not determined quantitatively [14]. To make a detailed comparison of C. elegans...

Ngày tải lên: 29/03/2014, 09:20

... USA 95, 6448–6453. 23 Noinville S, Bruston F, El Amri C, Baron D & Nicolas P (2003) Conformation, orientation, and adsorption kinetics of dermaseptin B2 onto synthetic supports at aqueous ⁄ ... structured and aggre- gated in water and shows b-sheet formation in phosphate buffer solutions [28,48]. Similarly, Drs S9 was shown to fold into a non-amphipathic a-helical conformation in tr...

Ngày tải lên: 23/03/2014, 07:20

... Caplan, M.J. (2001) Ion pumps in polarized cells: sorting and regulation of the Na,K -and H,K-ATPases. J. Biol. Chem. 276, 2 9617–29620. 30. Simon s, K. & Ikonen, E. (1997) Functional raft s in ... f the ER or on the extracellular side (Fig. 2). Constructs were restriction-mapped and sequenced to verify correctness of the fragments. GFP, optimized for maximal fluorescence [39]...

Ngày tải lên: 31/03/2014, 09:20

... L429 and Y77 in strand b4, and between P431 and the aliphatic part of Q80 side chain in the loop connecting strands b4 and b5. As mentioned above, conserved Q234 in nNOS and other GIQVD- and KXTQT-containing ... an extended conformation and form an additional b-strand that extends a pre-formed b-sheet, with the glutamine residue occupying an invariant position in the DYNLL1 bind...

Ngày tải lên: 29/03/2014, 09:20

... depends, at most, on the N-gram length and the the N-gram count itself. The values of the α, β, and γ parame- ters depend on the context w 1 . w n−1 . For each context, the values of α, β, and γ must ... and γ must be set to produce a normalized conditional probability dis- tribution. Additional constraints on the previous 1 For n = 2, we take the expression p(w n |w 2 . . ....

Ngày tải lên: 20/02/2014, 09:20

... The interaction was mapped onto the LRR and AXH domain of Anp32 and Atx1 respectively, and was shown to be stronger for expanded Atx1 [16,18]. The temporal and cell-specific expression pattern of ... )0.12 Backbone conformation )1.69 Procheck Ramachandran statistics (%) Most favored region 75.1 Additional allowed regions 23.8 Generously allowed regions 0.4 Disallowed regions 0.8 a Ca...

Ngày tải lên: 18/02/2014, 17:20

... N-II (residues 56–61) and region N-III (residues 80–85) (Figs 3B and 5A). Compared with TvuCMBP, EcoMBP and TliTMBP, the positions and the conformations of the three regions are strikingly different ... The conformations of Glc3 bound to TvuCMBP and the third glucose resi- due, Glc3¢, of maltotetraose bound to EcoMBP are superimposed well, and Glc2 and Glc4 also adopt sim- ilar...

Ngày tải lên: 19/02/2014, 00:20

... During storage for at least 1 month, no aggre- gates or monomeric c units were formed from pure c 11 ring preparations. However, after addition of isola- ted c monomers and incubation overnight, ... intact ones. Hence, intrinsic features of the I. tartaricus c subunits are responsible for the formation of c 11 oligomers in the fully assembled rings. Results and Discussion Synthesis a...

Ngày tải lên: 07/03/2014, 21:20

... that switches con- formations upon effector binding is in an identical conformation in both structures and resembles that observed for induced TetR (Fig. 1C). In this confor- mation, segment 100–105 ... unexpected. A central function of TetR is its ability to adopt different conformations. According to the conforma- tional switch model, TetR exists in two conforma- tions. In the ligand-fr...

Ngày tải lên: 16/03/2014, 00:20

... caused by conformational control of the bound sub- strate, which enforces a gauche(–) conformer for v 1 instead of the trans conformers observed for the asparagine oxygenase AsnO and other mem- bers ... mode results from conformational control of the enzyme on the side chain rotamer of the bound substrate. For example, in AsnO, a trans con- former is selected for the v 1 torsion...

Ngày tải lên: 16/03/2014, 02:20