Báo cáo khoa học: Classification of the short-chain dehydrogenase ⁄reductase superfamily using hidden Markov models potx
Báo cáo khoa học: Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains pdf
... due to the proximity of the negative c harge of the side chain of Glu706 from the neighboring subunit. The mutation of these residues might interfere with the oligo- merization required for the ... of the amino acid sequences of the majority of the currently known Lon proteases. The results of site-directed muta- genesis of E. coli Lon protease and insi...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx
... by the amniotic fluid pro- tease and suggest that not only the degree, but also the position of the phosphate groups influence the process. As for the other IGFBPs, the proteolytic cleavage of IGFBP-1 ... have examined the propensity of the different isoforms of IGFBP-1 to backbone cleavage in the presence of this partially purified specific protease. Results Purifica...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
... previously [18]. To assess the role of the S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions of the other subunits in a het- erooligomer, the corresponding ... In the lower panels, the mean values and standard error of mean values of relative specific radioactivity of the different constructs are plotted. The nu...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Identification of the heparin-binding domains of the interferon-induced protein kinase, PKR pptx
... indicate the positions of PKR mutants and the mutant name is shown at the bottom of the panels. (C) Dimerization activity of the point mutants. The ability of point mutants to dimerize was tes- ted using ... extracts (Fig. 6C) and all of the mutants were expressed at the same level in HT1080 cells. The kinase activity of the mutants was further tested by assa...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: "Classification of Feedback Expressions in Multimodal Data" pdf
... Looking at the cases of disagreement we could see that many of these are due to the fact that the annotators had forgotten to remove some of the features automatically proposed by ANVIL from the latest ... cannot see each other. Furthermore, the restricted contexts in which feedback expressions occur in these di- alogues allow for a very fine-grained analysis of the relat...
Ngày tải lên: 23/03/2014, 16:20
Báo cáo khoa học: "Classification of Semantic Relationships between Nominals Using Pattern Clusters" pdf
... for students and professionals ’, we use the symmetric pattern ‘X and Y’ to add the word scholarships to the group of loans and to add the word professionals to the group of students. We do not ... patterns are left but there are still several winning clusters, we assign to the pair the label of the cluster with the largest number of pattern instances in the corpus...
Ngày tải lên: 23/03/2014, 17:20
Báo cáo khoa học: Identification of the N-terminal region of TjZNT2, a Zrt⁄Irt-like protein family metal transporter, as a novel functional region involved in metal ion selectivity ppt
... with the exception of the N-terminus and the TM III–IV loop. To examine the functions of TjZNT1 and TjZNT2, these transporters were tagged with hemagglutinin (HA) at their N-termini to verify their ... region of the N-terminus that is important for the inhibition of Zn 2+ transport. Fur- thermore, the N-terminal truncated TjZNT2 lacked Mn 2+ uptake activity. These finding...
Ngày tải lên: 29/03/2014, 00:20
Báo cáo khoa học: Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx
... measuring the decrease of the enolic OAA absorbance at 280 nm [7]. The reported OAD activity was corrected considering the spontaneous decar- boxylation of OAA catalysed by the presence of the diva- lent ... One of them is encoded by the oad genes and is a homologue of the OAD membrane bound complex from Klebsiella pneu- moniae [21]. The other is coded by the cit...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf
... the rate of the conformational change, k 3H the hydride transfer and k 4 the rate of the product-off step. Hence, in these circumstances, the steady-state K M is expected to be smaller than the apparent ... malate dehydrogenase activity One of the more striking sequence differences between PfLDH and other LDHs of the same fold is the presence of a lysine residu...
Ngày tải lên: 23/03/2014, 09:20
Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx
... synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones confirms the severity of the thermal stress under our experimental condi- tions. A number of ... constitutive isoforms of HSP70 are synthesized in the gills and mantle of C. gigas [26]. The expression level of the constitutive forms increases after thermal stress, wher...
Ngày tải lên: 18/02/2014, 16:20