Báo cáo khoa học: Cytoskeleton-modulating effectors of enteropathogenic and enterohaemorrhagic Escherichia coli: Tir, EspFU and actin pedestal assembly pdf

... FEBS MINIREVIEW Cytoskeleton-modulating effectors of enteropathogenic and enterohaemorrhagic Escherichia coli: Tir, EspF U and actin pedestal assembly Kenneth G. Campellone Department of Molecular and Cell ... Dynamin is required for F -actin assembly and pedestal formation by enteropathogenic Escherichia coli (EPEC). Cell Microbiol 9, 438–449. Regulat...

Ngày tải lên: 29/03/2014, 09:20

... indicated as c¢ and c 552 , respectively. The arrow and arrowhead indicate the positions of the PHCP and PH c 552 proteins, respectively. The band denoted by the asterisk on the right-hand side is ... coli Escherichia coli DH5a was used for the maintenance and propagation of all plasmids. The E. coli RI89, RI242 and JCB387 strains were examined with regard to the synthesis of...

Ngày tải lên: 06/03/2014, 00:20

... in the levels of HSF1 and MTA1 (a component of the NuRD complex containing HDAC1 and HDAC2) proteins and to binding of HSF1 to MTA1 [78]. The complex is recruited to the promoters of estrogen-responsive genes ... consisting of multiple inverted repeats of the pentanucleotide sequence nGAAn. A single HSF of yeast can bind to heat shock elements that differ in the configurati...

Ngày tải lên: 18/02/2014, 04:20

... PUF-8, Drosophila and human Pumilio and yeast Puf3 recog- AB CD Fig. 6. Analysis of binding affinity between APUM-2 and the FAS-2 3¢ UTR transcripts with nucleotide substitutions upstream and down- stream of ... that of other well- characterized members of PUF family. These proteins must bind to transcripts in the plant and regulate their translation and ⁄ or turnover. A la...

Ngày tải lên: 18/02/2014, 06:20

... of VsEndA (Fig. 1). The theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA. Expression and purification From 7 L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and ... Biology Centre, Faculty of Science, University of Tromsø, Norway 2 Department of Molecular Biotechnology, Faculty of Medicine, University of Tromsø, Norway The marine and...

Ngày tải lên: 19/02/2014, 05:20

... large number of orthologs (Figs 2,9). This suggests an essential role for FMN despite the low affinity of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. ... protein is clearly a member of a superfamily of (S)- a-hydroxyacid dehydrogenases, and the coenzyme pattern of Idi-2 protein, where the roles of FMN and of N...

Ngày tải lên: 19/02/2014, 13:20

... Lankelma 1,2 1 Department of Molecular Cell Physiology, Institute of Molecular Cell Biology, Faculty of Earth and Life Sciences, Vrije Universiteit, Amsterdam, the Netherlands; 2 Department of Medical Oncology, ... target genes expressed [16], and also affects the type of cellular response that is evoked [17,18]. Activation of ERK is required for proliferation of fibroblasts...

Ngày tải lên: 19/02/2014, 16:20

... half-lives of 22 and 23 h for wild type GAT1 in the absence and presence of dMM, respectively, and of 12 and 5.5 h for the DDN and DDQ mutants, respectively. Fig. 7. Plasma membrane trafficking of GFP-tagged ... proteins with N-glycans of mature complex type and the small band to the proteins with N-glycans of mannose-rich type. All bands of the mutants had reduced mo...

Ngày tải lên: 19/02/2014, 17:20

... motif, Fig. 3. Model of SEA domain of MUC1. Ribbon representation of the modelled SEA domain of human MUC1. The model consists of three a-helices and six b-strands forming an a ⁄ b sandwich fold. The ... MUC3- SEA and MUC1FDTR ⁄ MUC12SEA glycoproteins showed a major M2-reactive and CT-2 reactive non- cleaved glycoform of about 105–120 kDa (Fig. 4A,C, MUC3SEA and MUC12SEA)...

Ngày tải lên: 19/02/2014, 18:20

... UK; 2 Department of Chemistry, University of Otago, Dunedin, New Zealand; 3 Department of Neurology, Medical School, University of Newcastle upon Tyne, UK The selective manipulation of the expression and ... mitochondria and cells. In addition to facilitating the development of antigenomic therapies, such a reagent might also be useful in investi- gating the rate of turnover...

Ngày tải lên: 20/02/2014, 11:20