Báo cáo khoa học: Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A docx

... +34 915 6424 31 Tel: +34 917 459 511 E-mail: mbruix@iqfr.csic.es(Received 12 January 2 010 , revised 11 March 2 010 , accepted 15 March 2 010 )doi :10 .11 11/ j .17 42-4658.2 010 .07649.xHuman dynein light chain ... values for the 20lowest-energy conformers of the best cluster are0. 71 ± 0 .11 A˚ for the backbone and 1. 11 ± 0.09 A˚ for the heavy atoms. The mean total energy of the ensemble is ) 814 6 kcalÆmol )1 , ... compilation ª 2 010 FEBS Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A Marı´aF. Garcı´a-Mayoral 1 ,Mo´nica Martı´nez-Moreno2, Juan...

Báo cáo khoa học: Structural basis for the erythro-stereospeciﬁcity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... 17 5 .1 1 91. 1 19 1 .1 Yes 3.40 ± 0.45 2 611 ± 19 6 767 ± 18 3D-Arginine 17 5 .1 1 91. 1 17 5.2 No – – –L-Homoarginine 18 9 .1 205 .1 205.0 Yes 7.05 ± 2.35 8 31 ± 16 6 11 8 ± 47 .1 L-Canavanine 17 7 .1 193 .1 193 .1 ... 17 .52 14 .18 13 .98No. of atoms 2954 3232 317 0No. of water molecules 288 4 51 3 81 No. of heterogens 13 10 12 rmsd from idealBond lengths (A˚) 0.008 0. 011 0. 010 Bond angles (°) 1. 13 1. 45 1. 36Torsions ... OccupancyVioC•L-arginine•Fe(II)•tartrate 17 3.9 12 6.3 16 8.8 )15 3.5 0.75 )15 9.9 73.7 11 8.9 76.4 0.25VioC•hArg )16 8.6 15 9.9 14 8.4 17 7.2 0.40 )16 3.0 90.0 12 1.7 71. 5 0.40VioC•hArg•Fe(II)•succinate )16 0.0 91. 2 12 6.6 60.8 0.70High-resolution...

Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt

... P2 1 2 1 2 1 P2 1 2 1 2 1 P2 1 2 1 2 1 P2 1 2 1 2 1 P2 1 2 1 2 1 P2 1 2 1 2 1 Beamline X 11 X 11 X 11 X 11 X 11 ID14 -1 Unit cell:a ¼ 56.86 A˚56.73 A˚57.28 A˚56.80 A˚56. 71 A˚56.78 A˚b ¼ 83. 21 A˚83.36 A˚82.68 A˚83 .14 A˚83.59 ... bindingTable 1. Data collection and reﬁnement statistics.Ligand-removed MannoseGlcNAc(b1–2)ManGlcNAc(b1–2)Man(a1–3)ManOMe M592 NA2FSpace group P2 1 2 1 2 1 P2 1 2 1 2 1 P2 1 2 1 2 1 P2 1 2 1 2 1 P2 1 2 1 2 1 P2 1 2 1 2 1 Beamline ... of the F and Y dihedral anglesdeﬁned as: F ¼ O5-C1-O1-Cx¢ and Y ¼ C1-O1-Cx¢-CC(x +1) for the b (1 2) and a (1 3) linkages. For a (1 6) link-ages, three dihedral angles F ¼ O5-C1-O1-C6¢, Y ¼ C1-O1-C6¢-C5¢-and...

Báo cáo khoa học: Structural basis for the changed substrate speciﬁcity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx

... 240Wildtypeb 1. 2 29.5 1 14.2 1. 2 · 10 7 1 dCN45D 0.9 3.4 1 1.6 1. 8 · 10 64N64D 11 8 3.4 0.8 1. 6 13 559 5 31 N45D ⁄ N64Da96.4 8.3 1 4.04 42000 17 1Wildtypeb2.3 34.2 1 16.5 7.2 · 10 6 1 dAN45D 11 9 3.8 ... 0 .15 6 1 0.076 38 605Wildtypeb665 31. 3 1 15 .1 23000 1 AZTN45D 11 .7 0.06 1 0.03 2564 1. 7N64D 11 .1 0.074 0.8 0.037 3333 1. 3N45D ⁄ N64Da7.2 0 .10 7 1 0.052 7200 0.6Wildtypea8.3 0.073 1 0.036 ... destabilizes the normal interactions with Glu1 71. In the dT complex, the negative charge of Asp64 repels Glu1 71 and the LID region becomes more ﬂexible and the part aroundGlu1 71 and 17 2 is not...

Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

... reﬂections 11 1 339 11 1 287 18 1 528Unique reﬂections 15 803 15 798 47 6 91 Completeness 96.7 (89.7)b96.5 (87.7)b 10 0.0 (10 0.0)bI ⁄ r(I) 43.5 (11 .5)b43.3 (11 .3)b24.0 (8.6)bRmerge(%)a 18 .7 ... and Glu3 61 form protru-sions at the entrance of the cleft, and the distance between Nf of Lys229 and Oe1 of Glu3 61 is 16 .7 A˚.On the other hand, the width of the sugar-binding cleftof EcoMBP ... segments (residues 12 8– 13 0, 280–282 and 3 31 333). The sugar-binding site islocated at a cleft formed by the two domains. Structural homology was searched for using the DALI server [25], and many bacterial...

Báo cáo khoa học: Structural basis for recognition of Co2+ by RNA aptamers pot

... RNA ligands,including the N7 atom of purines. Therefore, there is the possible involvement of the N7 atom of the neigh-boring tandem guanines, G27 and G28 or G37 and G38 in aptamer no. 18 , as ... (2008) 16 51 16 62 ª 2008 The Authors Journal compilation ª 2008 FEBS 16 53these purines are located in the single-stranded regionaccessible for chemical probing [19 ]. Thus, N7 atomsof the above ... intermediate. RNA 10 , 18 67 18 87.23 Kim SH, Shin WC & Warrant RW (19 85) Heavy metalion-nucleic acid interaction. Methods Enzymol 11 4, 15 6 16 7.24 Leontis NB & Westhof E (19 98) The 5S rRNA...

Báo cáo khoa học: Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase potx

... +4 21 2 59 41 0222Tel: +4 21 2 59 41 0275E-mail: chempbsa@savba.sk(Received 17 December 2 010 , revised 10 April 2 011 , accepted 13 April 2 011 )doi :10 .11 11/ j .17 42-4658.2 011 .0 812 7.xXylanase A from the ... xylanase A–ligand complexFEBS Journal 278 (2 011 ) 210 5– 211 6 Journal compilation ª 2 011 FEBS. No claim to original Slovakian government works 211 1(formerly GH5) family [1, 8 ,13 15 ]. These enzymes ... 4A,B. The Xyl in subsite )1 is also coordinatedwith Trp 113 , Asn164, and the catalytic Glu165 and Glu253 (Fig. 4B). The MeGlcA moiety interacts with the edges of the aromatic rings of the Trp289 and Tyr290...

Báo cáo khoa học: Structural basis for stereo-speciﬁc catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans pptx

... red for arginine, blue for glutamate and aspartate, green for histidine, cyan for polar, orange for aromatic and aliphatic side chains,brown for Thr77–Gly79 and dark red for Gly153-Asp176. The ... 533627 ⁄ 30959 714 20 ⁄ 19 159 715 81 ⁄ 19 152 60 515 ⁄ 19 338Completeness (%)a98.8 (93.9) 98.4 ( 91. 9) 98.4 ( 91. 8) 99.0 (97.8)Mean I ⁄ ra28 .17 (6.84) 18 .34 (6.84) 18 .43 (6.93) 16 .66 (6.80)Rsym(%)a5.3 ... GXGX2GX 17 D [GXGX2GX 17 D (15 3– 17 6) in HGDH; Fig. 1] and is therefore referred as the nucleotide-binding domain. The strictly conservedAsp176 is the key residue in the recognition and bind-ing...

Báo cáo khoa học: Structural basis for poor uracil excision from hairpin DNA An NMR study pptx

... G6(H1)–C 21 (H 41/ H42), G7(H1)–C20(H 41/ H42), A8(H2)–T 19 (H3), T9(H3)–A 18 (H2), C 10 (H 41/ H42)–G 17 (1NH) and C 11 (H 41/ H42)–G 16 (H1) establish a hydrogen bonded base-pairing between G6:C 21 (G6 and ... locked into t, g+ and t conformations, respectively, similar to the stem. On the other hand, for T 14 and T 15 ,thef is locked into g–conformation whereas the d adopts 14 8 and 11 0 ° onaverage, ... between G6:C 21 (G6 and C 21 ), G7:C20,A8:T 19 ,T9:A 18 ,C 10 :G 17 and C 11 :G 16 and hence the conformation ofstems of both of the hairpins. Qualitative analysis of the relative NOESY...

Báo cáo khoa học: Structural requirements for Caenorhabditis elegans DcpS substrates based on ﬂuorescence and HPLC enzyme kinetic studies pdf

... Km(min )1 Æmg )1 )Fluorescence methodm7GpppG m7GMP + GDP 1. 17 ± 0 .14 1. 53 ± 0 .11 1. 30 ± 0 .18 m7GpppA m7GMP + ADP 0.60 ± 0 .11 1. 09 ± 0 .11 1. 83 ± 0.38m7Gpppm6Am7GMP + m6ADP 1. 03 ... m6ADP 1. 03 ± 0 .16 1. 33 ± 0 .12 1. 30 ± 0.23m7Gpppm7Gm7GMP + m7GDP 1. 12 ± 0 .14 0. 91 ± 0 .10 0. 81 ± 0 .13 m7Gpppm2¢-OGm7GMP + m2¢-OGDP 1. 23 ± 0 .13 1. 66 ± 0 .12 1. 35 ± 0 .17 m7Gppp2¢dG ... Warsaw, PolandFax: +48 22 554 07 71 Tel: +48 22 554 0779E-mail: elab@biogeo.uw.edu.pl(Received 25 February 2 010 , revised 8 May2 010 , accepted 12 May 2 010 )doi :10 .11 11/ j .17 42-4658.2 010 .07709.xThe...

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