Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot

... Linewe- aver–Burk plots with 0–0 .05 m M and 0. 2–0 .35 mM substrate. Units of the ordinate and abscissa are lmolÆmin )1 and mM )1 , respec- tively; values correspond to the original plot. Derived values of ... bound as isolated in E 1 or modification of the inhibitor-binding site. A corresponding conforma- tional change at the cofactor-binding site was moni- tored from coe...

Ngày tải lên: 16/03/2014, 00:20

... [ 2–6 ]), however, it becomes apparent that the ligands ) including O 2 andCOtotheferrousformand H 2 O, OH – ,N 3 – and CN – to the ferric form ) cannot gain access to the closed haem pockets of ... pH 7.0 and 30 °C, indeed, Tyuma et al. [17] reported the P 50 values of 1.00 Torr for the a chains and 0.45 Torr for the b chains, whereas HbA showed P 50 ¼ 16.59 Torr in t...

Ngày tải lên: 17/03/2014, 10:20

... the transfer of energy from an excited donor fluorophore directly to an acceptor fluorophore, leading to an increased fluorescence emission of the acceptor and a quenching of the emission fluorescence of the ... the donor [5 2–5 4]. For FRET to occur, the donor and acceptor must have a sufficient spectral overlap, a favorable ori- entation and a separation of 1–1 0 nm [52]. B...

Ngày tải lên: 23/03/2014, 15:21

... the sharper the test of different perspec- tives. To account for large variation in the number of words and vocabulary size across corpora, we nor- malize the total number of words in a document collection ... number of documents |D|, average document length ¯ |d| , and vocabulary size V of the three corpora. The first perspective corpus consists of arti- cles published on th...

Ngày tải lên: 23/03/2014, 18:20

... Authors Journal compilation ª 2010 FEBS 1885 Purification of three aminotransferases from Hydrogenobacter thermophilus TK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis Masafumi ... the first report of the primary structure of bacterial gluta- mate:glyoxylate aminotransferase and alanine: glyoxylate aminotransferase, and...

Ngày tải lên: 29/03/2014, 09:20

... volume of 50 lL containing 0.75 lL of a template, 150 pmol of a forward primer (A), 150 pmol of a reverse primer (B or C), 1 · G-Taq buffer, 10 nmol each of dATP, dGTP, dCTP and dTTP, and 0.5 ... amino-acid sequences for designing degenerate primers for PCR amplification (data not shown). Cloning of anserinase cDNA PCR was performed using a set of primers (A and B) and...

Ngày tải lên: 19/02/2014, 07:20

... factor of 18.8% and an R free of 22.3% with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2). The resulting model is depicted in Fig. 2. The Ca backbones of ... [38] and in the CD-degrading TVA- II [13], but not in the CD-degrading enzymes ThMA and BaCD nor in the majority of a-amylases. Ca-II is located in the loop between a1anda2 of...

Ngày tải lên: 08/03/2014, 02:20

... of the epidermal growth factor receptor (EGFR) is dysregu- lated in various types of malignancy as a result of gene amplification, mutations, or abnormally increased ligand production. Therefore, ... S (2007) Effect of combining epidermal growth factor receptor inhibitors and cisplatin on proliferation and apoptosis of oral squamous cell carcinoma cells. Int J Oncol 30, 146...

Ngày tải lên: 16/02/2014, 09:20

... augmenter of liver regeneration (ALR) [16]. In 1996, the cDNA sequence of ALR was reported by Giorda et al. [17]. Unlike complete mitogens such as hepatic growth factor or transform- ing growth factor-a, ... pretreatment with a gradient of either CsA (0.1, 1.0, 10 or 15 lm ) or its analog NIM811 (0.1, 1.0, 10 or 15 l m) for 15 min, the cells were incubated with CCCP (30 lm) for...

Ngày tải lên: 16/02/2014, 09:20

... 2010 FEBS and size of adipocytes. A major role of adipocytes is to store large amounts of triglycerides during periods of energy excess and to mobilize these depots during periods of nutritional ... anti- (rabbit IgG) for 30 min (Vector Laboratories, Burlingame, CA, USA), and this was followed by staining with the avidin–biotin–peroxidase complex system (Vectastain ABC Kit; Ve...

Ngày tải lên: 16/02/2014, 09:20