Báo cáo khoa học: Sodium pump a1 and a3 subunit isoforms mediate distinct responses to ouabain and are both essential for survival of human neuroblastoma docx

... Sodium pump a1 and a3 subunit isoforms mediate distinct responses to ouabain and are both essential for survival of human neuroblastoma Larisa Karpova 1,2 , Alexander Eva 1 , Ulrike ... the a1ora3 subunit or through both subunit isoforms of the sodium pump. Ouabain- induced activation of Erk1 ⁄ 2 after silencing either a1ora3 subunits...

Ngày tải lên: 29/03/2014, 09:20

... Wang 1 and Dongyun Hao 1,2 1 Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, China 2 Biotechnology Research Centre, Jilin Academy of ... has been proven to be the recognition site of DRE-binding proteins, which are transcription factors that have authentic ERF domains [13] and that are involved in the ind...

Ngày tải lên: 18/02/2014, 13:20

... times of adenosine and adenine, inosine and hyp- oxantine, guanosine and guanine, uridine and uracil, cyti- dine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min, 15.2 and 6.1 min, 6.8 and ... [58,59] and FUGUE [60] and gave the same results, therefore confirm- ing the correctness of the chosen templates. The sequences of target and templates were aligned to...

Ngày tải lên: 18/02/2014, 17:20

... studies. Both were found thanks to their sequence similarity to other Y receptors, and the sequence comparisons sug- gested that both Y 6 and Y 7 arose before the radiation of gnathostomes in ... the epitope tag (not shown). To avoid having to rely on a high-affinity radioligand for deter- mination of the receptor’s pharmacological profile, we performed a number of funct...

Ngày tải lên: 19/02/2014, 07:20

... in the areas of morphology of the respiratory system, its adaptations and the physics of respiration [1–3]. By contrast, the molecular mechanisms of tracheole ventilation are poorly understood. Early ... dilution of biotinylated anti-rabbit IgG (Vector Laboratories) for 1 h. Tissues were washed 4 · 20 min in PBSTG and incubated in a 1 : 200 dilution of Texas Red- Streptavi...

Ngày tải lên: 20/02/2014, 23:20

... constitutive punctae formation and Ca 2+ influx. In addition, the grafted single EF-hand motif formed a dimer regardless of the presence of Ca 2+ , which conforms to the EF-hand paring paradigm. ... conformation, whereas the b-strand content largely remained similar to CD2.D1 (Fig. 1E). The isolated EF-hand binds to Ca 2+ and lanthanide ions One of the most important steps to...

Ngày tải lên: 07/03/2014, 00:20

... factor, hypoxia-inducible factor-1 (HIF-1), mediates essential homeostatic responses to reduced oxygen [43,44]. HIF-1 has been shown to mediate transcriptional acti- vation of HO-1 in a rat model of hypoxia [45] and ... phos- phatidylinositol 3-kinase (PI3K) pathway and its downstream effectors [23]. This pathway is a key sig- nal transducer of many growth factors and cy...

Ngày tải lên: 07/03/2014, 12:20

... checkpoints and it is involved in recruitment of checkpoint proteins to dynein [77]. Apoptosis/autophagy BimL and Bmf are BH3-only pro-apoptotic proteins thought to be normally sequestered to dynein and ... complex crucial for receptor immo- bilization at both excitatory and inhibitory synapses. Scaffold proteins constitute one major group of pro- teins present at the...

Ngày tải lên: 14/03/2014, 22:20

... loading controls, respectively, and also used to ascer- tain the purity of the nuclear and cytosolic preparations (A). Bands were scanned, quantified densitometrically and are expressed as arbitrary units ... values are depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA (B). Each point is the mean ± SEM of three sets of experiments. (C) Real-t...

Ngày tải lên: 16/03/2014, 02:20

... 3.5 · more expression of both the S688A (3.5 · for PCSK9 and 3.2 · for its propeptide, respec- tively; lanes 1A and 1B) and E690A PCSK9 mutants (3.4 · for PCSK9 and 3.5 · for its propeptide, respec- tively; ... cell cultures of HepG2, Huh7, HEK293 and CHOK1 cells untranfected and transfected with a hPCSK9 expression vector were collected and immuno- precipitated as...

Ngày tải lên: 16/03/2014, 06:20