Báo cáo khoa học: Mechanism for the hydrolysis of hyaluronan oligosaccharides by bovine testicular hyaluronidase docx

... January 2010) doi:10.1111/j.1742-4658.2010.07600.x Synthetic hyaluronan oligosaccharides with defined structures and their pyridylaminated derivatives were used to investigate the mechanism of hydrolysis of hyaluronan by bovine testicular hyaluronidase. ... to confirming the previously proposed mechanism for the hydrolysis of HA by BTH. AB C a b cd a b cd D...

Ngày tải lên: 29/03/2014, 08:20

... the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH13 enzymes, and especially the sequence of ... b-strands b2, b3, b4 and b8 of the (b ⁄ a) 8 barrel domain, and for rBAT also with the short stretch near the C-terminus of domain B [9,19]. From the sequ...

Ngày tải lên: 18/02/2014, 13:20

... respect- ively. On the other hand, the activity of cytochrome c reductase, still detected in the presence of antimycin A, could depend on the presence of a similar enzyme on the outer membrane of plant ... possess at the N-terminus the typical presequences of the chloroplastic protein transit peptide [28], similarly to what reported for pea ferritin [29,30]. On th...

Ngày tải lên: 16/03/2014, 18:20

... depends on the promoter context, which refers both to the specific arrange- ment of the cis-acting elements in the promoter and to the nature of the factors they bind. To investigate the effect of promoter ... combination of proteins by the value obtained when the fold induction for e ach of the proteins present in the combination were added (Table 1). There...

Ngày tải lên: 23/03/2014, 13:20

... 1.5 kb apart. We therefore analyzed the promoter function of the 1.5 kb 5¢-flanking region of the HO-2 gene in the present study. YN-1 cells were transfected with each construct of the HO-1 and HO-2 ... degrada- tion in the population of hemoglobin-positive cells. To the best of our knowledge, there has been no report on the promoter function of the human HO-2 Fig...

Ngày tải lên: 19/02/2014, 06:20

... then used for further analysis. In the reductive reaction, the Michaelis–Menten con- stant K m for DHA was 1 mm, and the K m for NADPH was 50 lm. The V max was estimated at 2400 nkat per mg of ... USA) three times for 30 s. The mixture was then centrifuged in an Eppendorf microcentrifuge at full speed for 25 min, and the supernatant used for the analysis. The pro...

Ngày tải lên: 19/02/2014, 06:20

... Examination of 50% of the data in the SLs leads to identification of between 75% (AdjN) and 80% (PNV) of the TPs. (ii) For the first 40% of the SLs, and lead to the worst results, with outperforming ... precision values above 50% for the first 10% of the list, but is outper- formed by the t-test afterwards. Looking at the first 40% of the data, there is a...

Ngày tải lên: 20/02/2014, 18:20

... which facili- tates their interaction with the anionic membranes of microbial cells, and they exert their antimicrobial action by the use of nonreceptor-based mechanisms of Keywords anionic; ... membranes, but, in the presence of the peptide, they are positive. These results confirm that E. coli membranes are thermodynamically less stable in the presence of AP1, further s...

Ngày tải lên: 07/03/2014, 12:20

... extracts instances of the “trouble” or “obstacle” relations from the Web in the form of pairs of fillers for these bi- nary relations. Their approach, which is described for the Japanese language, ... and the types of risk they are facing, together with bub- ble sizes proportional to the number of alerts that the Risk Monitor could discover in the corpus. The se...

Ngày tải lên: 17/03/2014, 00:20

... present ev- idence for a linguistically defined limit on the usefulness of features which is driven by the idiosyncratic properties of the verbs and the specific attributes of the desired verb ... classes. The pur- pose of the manual classification is (i) to evaluate the reliability and performance of the clustering experiments on a preliminary set of verbs,...

Ngày tải lên: 17/03/2014, 22:20