Báo cáo khoa học: 9-Deazaguanine derivatives connected by a linker to difluoromethylene phosphonic acid are slow-binding picomolar inhibitors of trimeric purine nucleoside phosphorylase potx

... The Authors Journal compilation ª 2010 FEBS 9-Deazaguanine derivatives connected by a linker to difluoromethylene phosphonic acid are slow-binding picomolar inhibitors of trimeric purine nucleoside phosphorylase Katarzyna ... Yatsu T, Hashimoto M, Hikishima S, Magnowska M, Bzowska A & Yokomatsu T (2008) 9-Deazaguanine derivatives: synthesis and in...

Ngày tải lên: 29/03/2014, 08:20

... International, Mon- treal, Canada). The signal was quantified by densitometry using Syngene’s Chemigenius 2XE imager and genetool software (VWR International). Untransfected and transfected HepG2 and ... sulfation of the prodomain, the common naturally occurring A5 3V variation that has no significant effect on plasma cholesterol levels and the R46L variation, a naturally occurring varia...

Ngày tải lên: 16/03/2014, 06:20

... CA, USA), zinc sulfate (Caledon Laboratory Chemicals, Georgetown, Canada), ammonium formate buffer (Sigma-Aldrich, Oakville, Canada), ammonium hydroxide (BDH Chemi- cals ⁄ VWR, Mississauga, Canada), ... divided by a factor of 33. UV spectra were acquired using a Cary 5G UV-Vis-NIR spectrophotometer (Varian Canada, Mississauga, Canada) in a 1 cm quartz cuvette at room temperature (22...

Ngày tải lên: 16/03/2014, 06:20

... terms of training set size. We want to remind the reader that our two algorithms are aimed at small datasets. We randomly split each dataset into 10 subsets where each subset was a test set and ... 2006). They used a natural language tagger which was trained on the output of ParaMor and Morfes- sor. The goal was to mimic each algorithm since ParaMor is rule-based and there is no...

Ngày tải lên: 20/02/2014, 04:20

... When a source docu- ment is available in multiple languages, a translator can benefit from the disambiguating information in each. Translator CT can take advantage of such in- formation without making ... work required to train and apply a syntax-aware statisti- cal machine translation system. 1 Introduction A parser is an algorithm for inferring the structure of its input, guide...

Ngày tải lên: 20/02/2014, 16:20

... sentences collected in Japanese are manually translated into English. We obtain a bilingual database. The database is called the ATR Dialogue Database(ADD). ATR aims to build ADD to one million words ... and also that nouns are similar to the extent that they share verbs. These automatic classification approaches are also partially successful. However, Hindle says that th...

Ngày tải lên: 20/02/2014, 21:20

... experiments, increasing amounts (5, 10, 50, 100 and 200-fold excess) of unlabeled visfatin–PPREwt (5¢-CAAT AC AGGGCAAAGATCATGGAAG-3¢) or visfatin–PPRE- mut (5¢-CAATAC AGGAAAAAGAAAATGGAAG-3¢) oli- gonucleotides ... through nicotinamide phosphoribosyltransferase activity. Diabetologia 52, 2455–2463. 13 Fukuhara A, Matsuda M, Nishizawa M, Segawa K, Tanaka M, Kishimoto K, Matsuki Y, Murakami M...

Ngày tải lên: 06/03/2014, 22:21

... conformational transitions linked to Na + binding Raimondo De Cristofaro 1 , Andrea Carotti 2 , Sepideh Akhavan 3, *, Roberta Palla 3 , Flora Peyvandi 3 , Cosimo Altomare 2 and Pier Mannuccio Mannucci 3 1 ... s.r.l., Cremella, Italy). The chromatographic peaks were analyzed and quantified using borwin-1 software (Jasco Europe). The results were expressed as a percentage with respect to t...

Ngày tải lên: 07/03/2014, 12:20

... presaturation to minimize any residual water and methanol signals. A total of 65 536 complex data points were recorded with a sweep width of 5531 Hz and 32 scans. The data was processed with a ... em including a vacuum degasser, quaternary HPLC pump, an autosampler, and a diode array detector. Chromatographic separation was carried out on a Phenomenex Prodigy ODS3 (5-lm part...

Ngày tải lên: 07/03/2014, 16:20

... seems to dominate. Among these are Ste24p a nd Axl1p involved in the maturation of the a- mating factor [6,7] and the mitochondrial signal peptid- ases, Mas1 and Mas2 [8]. We used human cholecystokinin ... H 2 Oandtransferredtoa 2 m L Eppendorf tube. An equal amount of acid washed glass beads (Sigma-Aldrich) was added, followed by 200 lLof0.1 M NaH 2 PO 4 (pH 4.5) including var...

Ngày tải lên: 07/03/2014, 16:20