Báo cáo khoa học: Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b doc

... transfection of sh1, sh2 and sh3 (Fig. S1A). Induction of gene expression of Snail, EGR-1 and SP-1 by TPA To investigate whether any other transcription factors cooperate with Snail for activation of the ... h of TPA treatment, EGR-1 is recruited to the same region and associates with SP-1. Subsequently (within 4–6 h of TPA treatment), Snail is also r...

Ngày tải lên: 29/03/2014, 08:20

... regulation of growth arrest and apoptosis [16], stimulation of cell proliferation [17] and protection against apoptosis [18]. Other S100 proteins also have proliferative and anti-apoptotic effects ... Dose- and time-dependence of induction of S100A8 mRNA by FGF-2 and heparin in confluent 3T3 cells. (A) Confluent 3T3 cells treated with FGF-2 (1.5 p M-15 nM) and heparin (1 I...

Ngày tải lên: 19/02/2014, 18:20

... Sharpe 2 and Ellen Solomon 1 1 Division of Medical and Molecular Genetics, and 2 Department of Craniofacial Development, GKT School of Medicine, Guy's Hospital, London, UK NBR1 (named as next to ... protein products of both pGBT9NBR1 333 and pGBT9NBR1 Cterm were able to interact with full-length NBR1 (see Fig. 1B, i,iii,iv and vi). FEZ1 and CIB interact with e...

Ngày tải lên: 08/03/2014, 10:20

... been found to be specific and potent HAD inhibitors. To check for covalent modifications of the enzyme, the effect of the inhibitor on the molecular mass value of HAD was measured; instead of an adduct ... lower with the top-down approach, and quantitation of multiply modified isomers is more efficient. Bottom-up proteolysis destroys the information on the size of the protei...

Ngày tải lên: 18/02/2014, 16:20

... sucrose and sorbitol). Counteracting osmolytes consist of the methylamine class of osmolytes, which are believed to have the special ability to protect intracellular proteins against the inactivation ... the presence of methylamines and sugar osmolytes. This study, in fact, has led to the classification of osmolytes into three different classes based on their effects on stabi...

Ngày tải lên: 07/03/2014, 00:20

... aim of the automatic-parsing component of a machine-translation program is the adequate recognition of the boundaries and functions of syntac- tic units. On the basis of this recognition, automatic ... alternatively with several of these grammar tables, provided the rule format is adhered to. The ad- vantage of this approach is supposed to be greater simplicity and...

Ngày tải lên: 07/03/2014, 18:20

... electron transfer reactions of cyt b 5 Phe35fiTyr and Phe35fiLeu variants with cytochrome c, with the wild-type and the Tyr83Phe, Tyr83Leu variants of plastocyanin, and with the inorganic complexes ... of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the wild-type cyt b 5 is 0.21 A ˚ , within the error limit. The side chain of Tyr35 of the Phe3...

Ngày tải lên: 08/03/2014, 10:20

... The degradation of IjBresults in the translocation of NF-jB to the nucleus and the transcriptional activation of target genes [30,31]. As LZK triggered the transcriptional activity of NF-jB, as ... cells [15, 37–39], and MLK2 and MLK3 play important roles in activation of the JNK pathway leading to neuronal apoptosis in response to kainate [36]. In contrast, the...

Ngày tải lên: 17/03/2014, 09:20

... in both constitutive and regulated splicing via binding to cis-elements of pre-mRNA or interaction with other splicing factors. The RS domain interacts with both proteins and RNAs [1–3]. In particular, ... inter- actions of SR proteins [1–5]. Reversible phosphoryla- tion of SR proteins is important for assembly and function of the spliceosome and for proper regulation o...

Ngày tải lên: 23/03/2014, 06:20

... & Ziparo E (1995) Inflamma- tory mediators increase surface expression of integrin ligands, adhesion to lymphocytes, and secretion of interleukin 6 in mouse Sertoli cells. Proc Natl Acad Sci ... Association of R4 transcriptional activity with a jB element. (A) Further map- ping of transcriptional activities to a subsec- tion (R4-1) of R4. The R4 sequence was arbitrari...

Ngày tải lên: 29/03/2014, 00:20