Báo cáo khoa học: The biochemical properties of the mitochondrial thiamine pyrophosphate carrier from Drosophila melanogaster ppt

... al. Mitochondrial transport of thiamine pyrophosphate FEBS Journal 277 (2010) 1172–1181 ª 2010 The Authors Journal compilation ª 2010 FEBS 1181 The biochemical properties of the mitochondrial thiamine pyrophosphate ... congested-like tracheaegene of Drosophila melanogaster encodes a member of the mitochondrial carrier family required for gas-ﬁlling of the tracheal system and expansion of the wings aftereclosion. ... mitochondria.Although most of these cofactors have to be imported from the cytosol into mitochondria, very little isknown about the molecular basis of their transportacross the mitochondrial membrane. Thiamine...

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... used for the quantiﬁcation of the enzymatically formed H2S.Measurement of pyruvate formation The activity of the puriﬁed d-CDes enzyme was followedthrough the measurement of the amount of pyruvateformed ... through an inhibition of threonine deaminase.On the other hand, the presence of the yedO genestimulates cell growth in the presence of d-cysteine assole sulfur source because the bacterium can ... anddecreased at the beginning of the light cycle. The d-CDes gene expression or the stability of the d-CDesmRNA was negatively regulated by light (Fig. 6A). The Western blot results using the d-CDes...

Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

... increased. The results suggestedthat the effect of salts on the oligomeric state of CDase I-5 correlated with the dissociation of the dodecameric form of the enzyme.DiscussionAn earlier study on the ... 7.0. The tertiary structure indicated that the effect of pH on dissociation of the oligomer was mainly due to the pro-tonation of H539. Unlike the pH-dependent process, the dissociation of wild-type ... identify the mechanism and forces that contribute to the supramo-lecular assembly and function of the enzyme. In addi-tion, the role of histidine residues at the interfaces in the formation of the...

Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf

... severalrepetitions of the motif. Repetition of the motif seems todetermine the speciﬁcity of binding to the target RNAsequence as well. Indeed, analyzing the PPR proteins of the Arabidopsis genome ... deletion of seven out of nine did not change the RNA-binding properties [41]. Deﬁning the exact structure of the HCF152 homodimer together with the petB precursor(unspliced) RNA will uncover the ... be located at the C-terminus of the protein although binding of the C-terminus half (CH) isonly half of the full-length protein (Fig. 3). Interestingly, the binding efﬁciency of the full-length...

Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf

... the projection of the tilt of the z axis on the x¢,y¢ plane (deﬁned direction of tilt of z and the x¢ axis), and j % a + c deﬁnes the location of the rhombic (x, y) axes. The orientation of the ... at position E7 as the source of the H-bond to ligand on the basis of apartial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree globin ... consistent with the % 10° tilt of the Fe-CN vector from the heme normal to avoid van der waalsoverlap with the Tyr32(B10) Og. The location of the rhombic axes, j % 50 ± 10°,isinagreementwiththeexpectations...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... positions of molecular mass markers are shown to the left,and the size in base pairs of the products to the right (the Meis2 equivalent of Meis3.2 would be expected at 149 bp). (C, D) RNA from aseries ... However, the activity of the full-length Meis1awas not tested, and on the basis of our work we expectthat its activity would be inhibited by the conservedHth domain. The Meis1a isoform, in which the C-terminal ... independently of Pbx, and does notpossess the autoinhibitory function of the Hth domain.In summary, our data suggest that one function of the conserved Hth domain is to inhibit the activity of the transcriptional...

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... of a severe mutant. At the otherend of the spectrum, where we have low energy, thereis 75% probability for the mutation to be nonsevere if the energy is 0.125 or lower. Thus, on the basis of ... algorithms have been developed recently [4–7]. The major drawback of these analyses is the lack of information regarding the activity or loss of activity of the target protein, as only a few variants ... activity) but, in all of these cases except for the 1% value, the data were always harder to separate (seeTable 3). In the case of the 1% limit, the distributionbetween the two classes is highly...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity of the pseudouridine synthase RluD in Escherichia coli doc

... obtained with the transition at posi-tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the othertwo ... bySouthern analysis. The rluD::cat strain had a full deletion of both rluD and yﬁH, together with their annotated pro-moter sequences. The inserted cat cassette had a distance of 90 nt from the ... dedicated to the memory of ProfessorJames Ofengand from the Univeristy of Miami. Wethank Dr U¨. Maiva¨li for critically reading the manu-script and Joachim Gerhold (both from Tartu Univer-sity)...

Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx

... Given that the roles of these residues deﬁnitely conﬁrm each other, the dif-ference in their signiﬁcance might be attributable to the importance and ⁄ or necessity of the receipt of the phenol-hydroxyl ... environments of BPA in the ligand-binding pocket of the ERRc. The proximity of each amino acid residue (within adistance of 5 A˚) to BPA is shown in the boxes depicting the a -heli-ces. The portrait ... for the major role of Arg316 in arresting the ligandWhen the amino acid sequences of the LBD of all the NRs were aligned to that of ERRc, it became notice-able that 26 receptors among the total...

Tài liệu Báo cáo khoa học: N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity ppt

... each extremity of the polypeptidechain, must of necessity arise from contacts that aremade outwith the active site of the enzyme. The effect of removal of the N-terminal extension The full N-terminal ... residues69–81 from the S. castellii sequence is small comparedto that achieved by location of the full-length extensionat the N-terminal end of the polypeptide. The beneﬁt of these alterations ... in the respective polypeptides werereplaced by their counterparts from the othersequence. The chimaera that consisted of residues 1–34 from S. cerevisiae IA3fused to residues 35–81 from the S....

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