Báo cáo khoa học: Application of the Fc fusion format to generate tag-free bi-specific diabodies doc

... mgÆL )1 culture, i.e. five times that of the secreted tag-free hEx3-Db. Thus, secretion of BsAbs as the Fc fusion format increased the amount of prepared tag-free diabodies due to the high produc- tivity (approximately ... mm gradient of NaCl in 50 mm phosphate solution (pH 6.0). Preparation of tag-free hEx3 diabodies from the Fc fusion format To c...

Ngày tải lên: 29/03/2014, 08:20

... per monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second DNA had less influence (Fig. 5A). To ensure that the estimation of the ... no tryptophan residue, and therefore, the fluorescence of these mutants corresponded to that of the inserted trypto- phan residue. The fluorescence peaked...

Ngày tải lên: 19/02/2014, 06:20

Ngày tải lên: 31/03/2014, 00:20

... IF 0 represents total concentration of the binding sites. Binding of IF to the receptor IF, with or without ligands, interacted with the specific receptor cubilin immobilized on the surface of the detect- ing ... organization of IF, where the Fig. 6. Interaction of IF with the receptor-coated BIACore chip in the presence or absence of the ligand. At time 120 s I...

Ngày tải lên: 19/02/2014, 05:20

... closure therapy Vacuum-assisted closure therapy (VAC Vakuumquellen, KCI Therapiegeräte, Höchstadt, Germany) was designed to pro- mote the formation of granulation tissue in the wound bed, either ... aftermath With respect to the tsunami's psychoemotional aftermath, the full impact of the wave on the mental health of the survivors is still unknown [2]. In February...

Ngày tải lên: 25/10/2012, 10:41

... as the ATP-unbound and Ca 2+ -bound E1Ca 2+ form. In the histogram (Fig. 2A) of the dis- tribution of the height of the projection of the embed- ded molecule above the flat membrane surface, the average ... in the range 10 n M to 100 lM. (B, C) Typical distributions of the lifetime of the elevated conformations of SERCA in the presence of 100 l M free...

Ngày tải lên: 14/02/2014, 14:20

... by T-pilus assembly. The assem- bled T4SS then triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of infected host cells or into the extracellular milieu. ... colonizes the surface area of the gastric mucosa in the human stomach and is one of the most adapted microbial pathogens.  50% of the world’s population carries t...

Ngày tải lên: 14/02/2014, 19:20

... 2). A first point of intervention within the biosynthetic pathway of ADAM10 is provided by directly interfer- ing with the expression of the gene for ADAM10: the promoter region of the gene for ADAM10 ... Acetylcholine esterase inhibitors, which are already used in the symptomatic treatment of AD, enhance the transport of ADAM10 to the cell surface and the non-a...

Ngày tải lên: 16/02/2014, 09:20

... which occurred either in the cells or during sample prepara- tion. The singly charged peptide with monoisotopic mass of 846.48, which corresponds to the predicted protonated mass of the myristoylated ... Notably, JSP1 seemed to stimulate preferentially the phosphorylation of the p46 isoform of JNK, whereas sorbitol treatment induced phosphorylation of both the p46...

Ngày tải lên: 16/02/2014, 14:20

... how the developmental activity of HSF1 is regulated. The identity of the developmental signal that promotes a monomer -to- trimer transition of HSF1 in the olfactory epithelium warrants further investigations. ... accordance with the fundamental role of HSF1 in the heat shock response, the require- ment of HSF1 and HSF4 in development of the lens and olfactory epit...

Ngày tải lên: 18/02/2014, 04:20