Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus pptx

... periodic acid ⁄ Schiff’s reagent; LAAO, L-amino acid oxidase; MRSA, methicillin-resistant Staphylococcus aureus; PSEM, Platichthys stellatus- derived epidermal mucus; psLAAO, LAAO sequence of Platichthys ... flounder LAAO-like antibacterial protein FEBS Journal 277 (2010) 453–465 ª 2009 The Authors Journal compilation ª 2009 FEBS 465 Novel L-amino acid oxid...

Ngày tải lên: 29/03/2014, 08:20

... aureus protects the bacterium from the host immune system and promotes its spread from the site of infection to other tissues and organs of the host. Indeed, FnBP-medi- ated invasion of endothelial ... antigenically distinct [10,11]. The A domain of ClfA and FnBPA bind Fg at the C-terminus of the c-chain [7]. The interaction between the A domain of ClfA and...

Ngày tải lên: 06/03/2014, 00:20

... good inhibitors of the asymmetrical hydrolases, having K i values within the range of the K m for Ap 4 A. By contrast, none of the six analogs were good substrates or inhibitors of the bacterial ... mode and in the case of the lupin enzyme it was over 20 times slower. Such slower cleavage to give 18 could arise either from weaker binding of 3 in the active site...

Ngày tải lên: 16/03/2014, 04:20

... From the present study, together with the similarity of EmbR with AfsR and other Regulation of EmbR activity by STPKs and Mstp K. Sharma et al. 2716 FEBS Journal 273 (2006) 2711–2721 ª 2006 The ... PknA ⁄ PknH inhibits their interaction with EmbR To further comprehend the role of Mstp, the effect of Mstp-mediated dephosphorylation of kinases on their specific inte...

Ngày tải lên: 16/03/2014, 14:20

... domain of PBP2 from Staphylococcus aureus [15,16] and of the Mtg from the same organism [17], the GTase module of PBP1a from Aquifex aeolicus [18,19], and the full-length PBP1b from Escherichia ... Interestingly, the solubilization efficiency was related to the length of the carbon chain: the shorter the chain, the better the solubilization. For instan...

Ngày tải lên: 29/03/2014, 21:20

... ion mass spectrum of the doubly charged ion at m ⁄ z ¼ 660.18. The isotopic distribution of the b 2 ion (inset) indicates the presence of bromine. The MS ⁄ MS spectrum allows assignment of the sequence SW*NCYNGHCTG, ... replacement of a proton on the c-carbon of a Glu residue with a CO 2 molecule [13]. The c-glutamyl carboxylase is the sole enzyme known to use...

Ngày tải lên: 23/03/2014, 11:20

... sequence, the typ- ical HSE contains at least three nGAAn units [4]. The helical repeat of the DNA (10.5 bp), the length of the unit (5 bp) and the inverted nature of the repeats, posi- tions all of the ... to the third A 3 . The remaining contacts with the DNA are to the phosphate backbone. Consistent with the structural data, in vitro-binding assays hav...

Ngày tải lên: 18/02/2014, 04:20

... assembly of TNSALP in the endoplasmic reticulum (ER), resulting in the loss of functional TNSALP from the cell surface [10–14]. Dele- tion of T at position 1559 of the cDNA [TNS- ALP (1559delT)] of the ... According to the recommended nomenclature, the adenine of the initiator ATG codon in the cDNA of TNSALP is denoted as nucleotide +1 instead of the first...

Ngày tải lên: 19/02/2014, 17:20

... concentrations of glucose, explore the molecular mechanisms of glucotoxicity in diabetes, and define the critical role of NOX2-derived ROS in the dysfunction and apoptosis of b-cells induced by d-glucose. There ... expression of cytochrome c and its release from mitochondria to the cytoplasm (Fig. 4C), resulting in activation of caspase-3. These observations suggest that...

Ngày tải lên: 06/03/2014, 02:20

... complex. Aggregation of the fragment was deduced from the turbidity of the last eluted fraction (Fig. 1Bd). This would account for the low recovery of soluble OA complex of fragment 53–103. For the sake of compari- son, ... and these signifi- cantly modify the physical state of the fatty acid in solution. The increase in the a-helical structure in the fragme...

Ngày tải lên: 06/03/2014, 09:22