Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the ﬂounder Platichthys stellatus pptx

... periodic acid ⁄ Schiff’s reagent; LAAO, L-amino acid oxidase; MRSA, methicillin-resistant Staphylococcus aureus; PSEM, Platichthys stellatus- derived epidermal mucus; psLAAO, LAAO sequence of Platichthys ... ﬂounder LAAO-like antibacterial proteinFEBS Journal 277 (2010) 453–465 ª 2009 The Authors Journal compilation ª 2009 FEBS 465 Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant ... with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the ﬂounder Platichthys stellatus Kosuke Kasai1,2, Takashi Ishikawa1, Takafumi...

Báo cáo khoa học: The A domain of ﬁbronectin-binding protein B of Staphylococcus aureus contains a novel ﬁbronectin binding site pdf

... aureus protects the bacterium from the host immune system andpromotes its spread from the site of infection to othertissues and organs of the host. Indeed, FnBP-medi-ated invasion of endothelial ... antigenically distinct [10,11]. The A domain of ClfA and FnBPA bind Fg at the C-terminus of the c-chain [7]. The interaction between the A domain of ClfA and the c-chain of Fg has beenstudied in detail. ... [15]. They are located in the equivalent positions to the aforementioned residuesP336 and Y338 of ClfA. One of the objectives of thisstudy was to determine the mechanism of Fg bindingby the...

Báo cáo khoa học: Novel diadenosine polyphosphate analogs with oxymethylene bridges replacing oxygen in the polyphosphate chain pdf

... good inhibitors of the asymmetrical hydrolases, having Kivalues within the range of the Kmfor Ap4A. By contrast, none of the six analogs were good substrates orinhibitors of the bacterial ... mode and in the case of the lupin enzyme itwas over 20 times slower. Such slower cleavage to give18 could arise either from weaker binding of 3 in the active site of the hydrolase in the reverse ... ab,a¢b¢-disubstituted analogs of Ap4A (e.g. ApCHFppCHFpA was cleaved at 3% of the rate of AppppA) [22]. They constitute a symmet-rical mode of cleavage of 5 by water attack at Pb. The failure of these hydrolases...

Báo cáo khoa học: EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine⁄threonine kinases and phosphatase in Mycobacterium tuberculosis doc

... From the present study, together with the similarity of EmbR with AfsR and otherRegulation of EmbR activity by STPKs and Mstp K. Sharma et al.2716 FEBS Journal 273 (2006) 2711–2721 ª 2006 The ... PknA ⁄ PknH inhibits their interaction with EmbRTo further comprehend the role of Mstp, the effect of Mstp-mediated dephosphorylation of kinases on theirspeciﬁc interaction with endogenous substrate, ... alone (lane 5) or in the presence of GST(lane 4). Therefore, the complex was formed only via the phosphorylated form of PknA. The absence of recovery of EmbR upon pre-incubation with dephos-phorylated...

Báo cáo khoa học: Optimization of conditions for the glycosyltransferase activity of penicillin-binding protein 1a from Thermotoga maritima ppt

... domain of PBP2 from Staphylococcus aureus [15,16] and of the Mtg from the same organism [17], the GTase module of PBP1a from Aquifex aeolicus [18,19], and the full-length PBP1b from Escherichia ... Interestingly, the solubilization efﬁciencywas related to the length of the carbon chain: the shorter the chain, the better the solubilization. For instance, the solubilization efﬁciency of Tm-1a* with ... effect of the domains. The thermal stability of CYMAL-4-puriﬁed Tm-1a*also displays two thermal transitions at the highervalues of 79 ± 1 and 89 ± 1 °C. These values arecompatible with a thermophilic...

Báo cáo khoa học: Novel c-carboxyglutamic acid-containing peptides from the venom of Conus textile docx

... ionmass spectrum of the doubly charged ion at m ⁄ z ¼660.18. The isotopic distribution of the b2ion (inset)indicates the presence of bromine. The MS ⁄ MSspectrum allows assignment of the sequenceSW*NCYNGHCTG, ... replacement of a proton on the c-carbon of aGlu residue with a CO2molecule [13]. The c-glutamylcarboxylase is the sole enzyme known to use vitamin Kas a cofactor. Carboxylation of Glu in the nascentpolypeptide ... neuronsand it has been suggested that the presence of the cCCS motif is involved in their biological activity [1]. The predicted modular structure of the precursorforms of Gla(1)–TxVI, Gla(2)–TxVI ⁄...

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... sequence, the typ-ical HSE contains at least three nGAAn units [4]. The helical repeat of the DNA (10.5 bp), the length of the unit (5 bp) and the inverted nature of the repeats, posi-tions all of the ... to the third A3. The remaining contacts with the DNA are to the phosphate backbone. Consistent with the structural data, in vitro-binding assays haveshown that the order of importance of the ... modules: the DNA-binding domain (DBD)at the amino-terminus and the oligomerization domainin the central region of the protein [1,4]. The HSFDBD belongs to the ‘winged’ helix-turn-helix family of DNA-binding...

Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspeciﬁc alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf

... assembly of TNSALP in the endoplasmic reticulum (ER), resulting in the loss of functional TNSALP from the cell surface [10–14]. Dele-tion of T at position 1559 of the cDNA [TNS-ALP (1559delT)] of the ... Accordingto the recommended nomenclature, the adenine of the initiator ATG codon in the cDNA of TNSALP isdenoted as nucleotide +1 instead of the ﬁrst nucleotide of the cDNA clone originally isolated ... intoCOP II vesicles at the exit site from ER and therefore the mutant protein remains longer in the lumen of the ER and ﬁnally is diverted to the degradation pathway.Because the degradation is blocked...

Báo cáo khoa học: Suppression of NADPH oxidase 2 substantially restores glucose-induced dysfunction of pancreatic NIT-1 cells doc

... concentrations of glucose, explore the molecularmechanisms of glucotoxicity in diabetes, and deﬁne the critical role of NOX2-derived ROS in the dysfunctionand apoptosis of b-cells induced by d-glucose.There ... expression of cytochrome c and its release from mitochondria to the cytoplasm (Fig. 4C), resulting inactivation of caspase-3. These observations suggest thatp38MAPK and p53 mediate the apoptosis of ... dysfunction of b-cells, we ﬁrst identiﬁed the source of ROS generated in response to d-glucose bydetermination of the effects on d-glucose-induced ROSlevels of various inhibitors of ROS-generating...

Báo cáo khoa học: The oleic acid complexes of proteolytic fragments of a-lactalbumin display apoptotic activity pdf

... complex.Aggregation of the fragment was deduced from the turbidity of the last eluted fraction (Fig. 1Bd). Thiswould account for the low recovery of soluble OAcomplex of fragment 53–103. For the sake of compari-son, ... and these signiﬁ-cantly modify the physical state of the fatty acid insolution. The increase in the a-helical structure in the fragment species upon addition of OA (Fig. 2) derives from the ... properties in the presence of a-LA orits fragments. With a decrease in the CAC of the fatty acid in the presence of the protein or its fragments,soluble and smaller aggregates of protein–OA...

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