... position of its initial encounter with the RCL to the other pole of the serpin [7– 10]. The translocation results in distortion of the proteinase [11] and inactivation of the enzymatic machinery ... effect on the conformation of b strand 5A and of the buried cluster in positions 53, 56, and 334. In contrast, in the presence of vitronectin, the K335Asubstitution caused a twofold to fivefold ... effects of the K335A substitution in the absence and presence of vitronectin, we propose that the conformational change of PAI-1 following the binding of vitronectin implicates a reorientation of the...