Báo cáo khoa học: The mitochondrial protein frataxin is essential for heme biosynthesis in plants potx

... exists on the role of this protein in the biogenesis of heme- containing proteins in plants. To gain insight into this process, we decided to study the role of frataxin using the enzyme catalase as ... heme- containing proteins [25]. Although the participation of frataxin in delivering iron to heme synthesis is frequently mentioned in the lit- erature, scarce...

Ngày tải lên: 28/03/2014, 23:20

... 2). DNA-binding activity of the mutant RecD proteins To further examine whether the loss or reduction in activities of the mutant RecD proteins are due to their inability to bind DNA, the binding ... towards gaining an insight into why RecD is essential in P. syringae, we have analyzed the in vitro biochemical activities of this protein. We report here the comparative a...

Ngày tải lên: 30/03/2014, 04:20

... of the ATP bind- ing domain is mutated to arginine, still interacted with PTI1-4. These data indicate that the kinase activity of OXI1 is not required for the interaction with PTI1-4. OXI1 interacts ... protein kinase; MAPKK, mitogen-activated protein kinase kinase; MBP, myelin basic protein; OXI1, oxidative signal-inducible 1; PDK1, 3-phosphoinositide-dependent kinase 1; PTI...

Ngày tải lên: 14/02/2014, 19:20

... with the N-terminal domain, MA, associated with the inner layer of the viral membrane, and the remaining domains forming the innermost protein layers accor- ding to their positions in Gag: MA, CA-NTD, CA-CTD, ... protein (MA; the Gag N-terminal domain), remains associated with the viral membrane, forming a discontinuous inner layer. The nucleocapsid protein (NC) remain...

Ngày tải lên: 18/02/2014, 06:20

... paid during folding of both the CAI peptide and a-helix 9 of CTD during formation of the peptide– protein and protein protein complexes. These NMR experiments also allowed the determina- tion of the ... 6110–6117 ª 2009 The Author Journal compilation ª 2009 FEBS Gag polyprotein, yields the matrix protein (MA), the capsid protein (CA), the nucleocapsid protein (NC) an...

Ngày tải lên: 18/02/2014, 06:20

... parameters describing the lipid ⁄ protein interface [23] remaining constant. The curves for the nAChR shown on the right were calculated as described in the Results. The following fitted curves to the data points ... uniform [8]. By contrast, there is evidence for special lipid-binding sites having high structural specificity, or dominating the lipid dependence of func...

Ngày tải lên: 19/02/2014, 18:20

... of multispan- ning membrane proteins, we have investigated the mechan- ism of how the sensor kinase protein KdpD inserts into the membrane. We found that KdpD inserts into the membrane independently ... KdpD-N. The protein was not accessible to the externally added proteinase K, indicating that it remains in the cytoplasm. Intriguingly, the formation of the dimeric for...

Ngày tải lên: 20/02/2014, 11:20

... structural changes in the CA-CTD, as is evident by the inability to form a C198-C218 disulfide bond, which is surprising consid- ering that 87 HAGPIA 92 is located in the CA-NTD [46]. The latter result ... to rescue infectivity [75]. In the TRIM–CyPA protein, CyPA replaces the PRYSPRY domain, but maintains the same function because the CyPA domain of TRIM5a–CyPA b...

Ngày tải lên: 07/03/2014, 00:20

... chains. Another interesting outlook is the identification of a putative cytosolic binding partner of the ISP, e.g. by using chemical cross-linking approaches and MS, giving interesting insights into ... membrane insertion. This is a remarkable find- ing in the light of the naturally occurring KR motif in plant ISPs. This observation raises the question whether the Tat transl...

Ngày tải lên: 07/03/2014, 11:20

... is localized on the tip of the FAD-binding domain, in close spatial proximity to the protein region encoded by exon VIa of the mSMOl isoform. This observation has led us to hypothesize that these ... between the two isoforms is the presence of an extra protein domain in mSMO l, encoded by the exon VIa [10]. Comparative analysis of the amino acid sequence of the v...

Ngày tải lên: 07/03/2014, 17:20