... efficient in catalyzing the transamination of a number of amino acids, including glutamine, phenylalanine, leucine, kynurenine, methionine, tyrosine, histidine, cys- teine, and aminobutyrate (Table ... I/glutamine transaminase K (EC 2.6.1.64; KAT-I) is a multifunctional enzyme. In vitro, the enzyme catalyzes the t ransamination o f several amino acids (e.g. glutamine, methion...
Ngày tải lên: 07/03/2014, 16:20
... were used as primary antisera (Table 3). Secondary antibodies (anti-rat IgG, anti-guinea pig IgG and anti-rabbit IgG; all raised in goat and conjugated to alkaline-phosphatase; Sigma, Saint Louis, ... dispersed in the X organ (Fig. 3A) , most likely as a result of arte- factual displacement of the cell bodies during the prep- aration of the organ. In the sinus gland, d...
Ngày tải lên: 18/02/2014, 11:20
Báo cáo khoa học: Predicting the substrate specificity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit pptx
... Fragariaxananassa UGT78D1 A. thaliana UGT8 6A1 A. thaliana UGT8 7A1 A. thaliana UGT8 3A1 A. thaliana UGT8 2A1 A. thaliana UGT8 5A1 A. thaliana SbHMNGT S. bicolor UGT76D1 A. thaliana UGT76E1 A. thaliana S39507 ... thaliana OsSGT1 O. sativa UGT74F1 A. thaliana UGT74F2 A. thaliana NtGT2 N. tabacum UGT75C1 A. thaliana UGT75B1 A. thaliana UGT75D1 A. thaliana UGT84B1 A. thaliana UGT8...
Ngày tải lên: 28/03/2014, 23:20
Tài liệu Báo cáo khoa học: Structural and functional specificities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx
... domain and the final 30 residues in the C-terminal end of the growth factor domain. These splice variants are also present in human thyroid papillary carcinomas (L. J. Reigstad, J. E. Varhaug and ... brain and in the adult brain it was expressed in several anatomical nuclei. PDGF-D mRNA is expressed in the developing rat eyes and also adult eyes of rat, cow, monkey and...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Casein kinase 2 specifically binds to and phosphorylates the carboxy termini of ENaC subunits ppt
... study phosphorylation of bENaC, oocytes were injected with a cRNA mixture containing a, c, and a hemagglutinin A (HA)-tagged bENaC. The HA epitope was introduced in the ecto domain at a position shown ... one shown in A) . (C) GST fusion proteins containing the C termini of a, b,andcENaC were phosphorylated by fraction 76. Autoradiogram and Coomassie blue staining are...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu Báo cáo khoa học: "Predicting the fluency of text with shallow structural features: case studies of machine translation and human-written text" doc
... high fluency. Results are shown in Table 3. Overall the best classifier is the multi-layer perceptron. On the task using all available data of machine and human translations, the classification accuracy is 86.99%. ... Head- line generation based on statistical translation. In Proceedings of the 38th Annual Meeting of the As- sociation for Co mputational Linguistics. R. Barz...
Ngày tải lên: 22/02/2014, 02:20
Báo cáo khoa học: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt
... traffic pass- ing through the main entrance. In mutants Trp83Ala and Trp83Glu, ligands that close the main entrance do not inhibit substrate hydrolysis because the traffic can pass via an alternative ... binding of substrate at the peripheral site (K p ), the rate constant for deacetylation (k 3 ) and the acceleration of deacety- lation (a) . Any other assumption resulted...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: "Predicting Strong Associations on the Basis of Corpus Data" pdf
... predict a larger va- riety of associations. Most computational models of semantic rela- tions aim to model semantic similarity in particu- lar (Landauer and Dumais, 1997; Lin, 1998; Pad ´ o and Lapata, ... expressed as the median and mean rank of the strongest association in this list. Associations absent from the list auto- matically received a rank of 101. Thus, the...
Ngày tải lên: 08/03/2014, 21:20
Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... kinetic para- meters of the PA-catalysed hydrolysis of the studied phenylacetyl arylamides are compared in Table 2. The substrates are arranged in a decreasing order of the ratio of their specificity ... protein chain, acting as a flap, first opening and then closing the groove [45]. The catalytic reaction presumably occurs when the ionized carboxyl group of PG...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx
... reference offset was set at 20%. Data evaluation, integration, analysis, and determination of binding parame- ters were performed using origin 5.0 software (Microcal, Inc.). Final protein and ligand ... in proteins and contains a number of transcription factors as well as protein kinases in its catalog of known substrates. Fundamental to signal transduction research is the under...
Ngày tải lên: 16/03/2014, 22:20