Báo cáo khoa học: Ablation of Sax2 gene expression prevents diet-induced obesity docx

... 2010 FEBS Ablation of Sax2 gene expression prevents diet-induced obesity Ruth Simon 1,2 , Stefan Britsch 2 * and Andrew Bergemann 1 * 1 Department of Pathology, Mount Sinai School of Medicine, ... 14; Sax2 ) ⁄ ) , n = 16; Sax2 + ⁄ + ⁄ Sax2 ) ⁄ + , P < 0.05; Sax2 + ⁄ + ⁄ Sax2 ) ⁄ ) , P < 0.0005 and Sax2 ) ⁄ + ⁄ Sax2 ) ⁄ ) , P < 0.005; males: Sax2 +...

Ngày tải lên: 28/03/2014, 23:20

... inducer of uPA gene expression [16]. Decma-induced uPA gene expression is dependent on Erk activation We and others have shown that activation of Erk plays an important role in uPA gene expression ... phos- phorylation. Moreover, expression of silent mutants of Shc isoforms showed that only p46 Shc and p52 Shc res- cued the effect of the siRNA. Overexpression of p66...

Ngày tải lên: 19/02/2014, 05:20

... on ASS gene expression via a decrease in the nuclear amount of NF-jB [44]. This illustrates that glutamine may regulate expression of the same gene via different pathways as a function of cell ... Analysis of gene expression in homocysteine- injured vascular endothelial cells: demonstration of GRP78/BiP expression, cloning and characterization of a novel reducing agen...

Ngày tải lên: 07/03/2014, 00:20

... nucleus. Downregulation of STAT3 expression resulted in reduction of the activation of the hsp70 promoter by Hsp105b. Furthermore, downre- gulation of Hsp105b reduced the expression of Hsp70 in heat-shocked ... suffi- cient for the Hsp105b-induced expression of the hsp70 gene. The promoter region between )206 and )187 bp of the hsp70 gene is essential for activation of...

Ngày tải lên: 18/02/2014, 06:20

... measure fold change of gene expression in knockout mice. Expression values were calculated using the Robust Multi-Array Analysis (RMA) plugin [13] of the genespring 7.3 software (Agilent Technologies, ... microarray analysis of gene expression in liver comparing wild-type and Tm7sf2 () ⁄ )) mice has been performed. Results Identification of mouse Tm7sf2 gene Genomic clones...

Ngày tải lên: 23/03/2014, 06:20

... involvement of the O-glycosylation of Sp1 in the induction of SREBP-1a transcription by glutamine. The gene expression of SREBP-1c, which represented less than 20% of the SREBP-1a gene expression ... in the induction of SREBP-1a gene expression. When endogenous Sp1 expression was reduced to  20% of normal with gene- specific small interfering RNA (siRNA), the gene...

Ngày tải lên: 28/03/2014, 22:21

... for in vivo imaging of gene expression of endoge- nous pri-miR9 during neurogenesis. To image in vivo the endogenous expression of the pri-miR9 in small animals, 2.5 · 10 6 of P19 cells bear- ing ... level of mature miR9 than of mature miR9*. In vivo visualization of unbalanced expressions of mature miR9 and miR9* during neurogenesis To monitor in vivo the endogenously u...

Ngày tải lên: 18/02/2014, 17:20

... because of the lack of Ab central region. In the case of Tk-RNase HII m–n – Ab 10)24 , the proteins are not aggregated efficiently, because of the absence of an Ab C-terminal region. Conformation of ... Corp., Poway, CA, USA). A total of 180 images were recorded with an exposure time of 5 s per image and an oscillation angle of 1°. Processing of the diffraction images and s...

Ngày tải lên: 07/03/2014, 12:20

... detachment of the metal from the protein has been achieved only after the addition of excess potassium cyanide. The implications of the present results for the pharmacolo- gical activity of these ... and were the subject of further biochemical and pharmacological inves- tigations [1]. Studies of the interactions of these gold(III) complexes with DNA, the classical target of pl...

Ngày tải lên: 07/03/2014, 21:20

... negative controls, instead of cell lysate, 10 lL of 0.10 lm E-64 or 10 lL of 0.01% Brij-35 was used. The mixture was allowed to incubate for 10 min before the addition of 7 lL of substrate (Z-Phe-Arg-AMC; 200 ... for 10 min before the addition of 7 lL substrate (Z-Phe-Arg- AMC; 200 lm). As positive and negative controls, instead of cell lysate, 5 lL of 0.10 lm E-64 or 5 lL of...

Ngày tải lên: 16/03/2014, 06:20