Báo cáo khoa học: Characterization of monomeric substates of ascorbate oxidase docx

... desensitization of the responses. (Lower) Responses of the three xTRHR subtypes to different concentrations of TRH. The maximum current amplitude of each recording was meas- ured and reported as a function of ... Amplification of cDNA templates with a set of primers corresponding to the Xenopus EF1a elongation factor cDNA (280 bp) as internal control of the poly(A) + RNA. (C) Ti...

Ngày tải lên: 21/02/2014, 03:20

... JHIII in any of the cell fractions. Transcriptional activation of DmEH with clofibrate Northern blot analysis revealed that transcription of DmEH in larvae was enhanced within 5 h of clofibrate ... Alignment of the five microsomal EH sequences. The deduced amino acid sequence of DmEH was compared with those of four other microsomal residential EHs. The abbreviations and accession n...

Ngày tải lên: 07/03/2014, 21:20

... 2007 FEBS Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima Mark Levisson, John van der Oost and Serve ´ W. M. Kengen Laboratory of Microbiology, ... The model covered most of the C-terminal part of EstD. The structure showed an a ⁄ b-hydrolase fold and indicated the presence of a typical catalytic triad consisting of a se...

Ngày tải lên: 23/03/2014, 09:20

... napus. The stability of AtNIT1 could be signi®cantly i mproved by t he addition of dithiothreitol and E DTA. The substrate range of AtNIT1 diers considerably from those of bacterial nitrilases. Aliphatic ... with 3-indolylacetonitrile, the rate of hydrolysis of 3-phenyl- propionitrile is increased by a factor of 330, and the K m value is reduced by a factor of 23. With the ex...

Ngày tải lên: 23/03/2014, 21:21

... region of the cardosin A gene allowed the identification of a region of approximately 500 bp essential for gene expression in transgenic flowers. Additionally, the relevance of the lea- der intron of ... regions of the genes fused to the reporter. Each row of panels represents independent flow- ers of plant lines transformed with the same construct, at different stages of devel...

Ngày tải lên: 30/03/2014, 09:20

... Characterization of monomeric substates of ascorbate oxidase Almerinda Di Venere 1,2, *, Eleonora Nicolai 1,2, *, Nicola Rosato 1,2 , ... properties of ascorbate oxidase. FEBS J 273, 5194–5204. 9 Avigliano L & Finazzi-Agro’ A (1997) Biological Func- tion and Enzyme Kinetics of Ascorbate Oxidase. In Multi-copper Oxidase (Messerschmidt ... high- pressure cell. The...

Ngày tải lên: 28/03/2014, 23:20

... 10 6 mL )1 . Next, 0.5 mL of propidium iodide staining solution (10 mg of propidium iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ P i ) was added to 0.5 mL of cell suspension. The ... were analysed in terms of production ⁄ consumption of TG. In mammalian cells, TG may be deleted to meet the needs of cell metabolism, either as a source of energy or as a res...

Ngày tải lên: 18/02/2014, 13:20

... C-terminal sequence of about 90 amino acids of unknown function. The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden- tity) argues for a conservation of the tertiary ... multiplicity of CLPs in humans and insects [14], we hypothesized that homologues of the previously characterized Cg-Clp1 remain to be found in C. gigas. In this article, we report t...

Ngày tải lên: 19/02/2014, 00:20

... followed by mixture with 6 lL of Lipofectamine Plus reagent at room temperature for 10 min. Four microliters of Lipofectamine Plus reagent were diluted in 100 lL of Opti-MEM I-reduced serum med- ium. ... Department of Legal Medicine and Medical Genetics, Gunma University, Japan 2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan 3 Division of Legal Medici...

Ngày tải lên: 19/02/2014, 06:20

... the mechanism of action of PPI1. The analysis of the pH dependence of PPI1- induced activation of the H + -ATPase showed that stimulation decreases dramatically with the increase of pH above pH ... with PPI1 588 His 6 . The analysis of the effect of PPI1 588 His 6 on the dependence of PM H + -ATPase activity on the concen- tration of MgATP (Fig. 3) showed that stimulation...

Ngày tải lên: 19/02/2014, 07:20