Báo cáo khoa học: Flavogenomics – a genomic and structural view of flavin-dependent proteins ppt

... FEBS REVIEW ARTICLE Flavogenomics – a genomic and structural view of flavin-dependent proteins Peter Macheroux 1,2 , Barbara Kappes 3 and Steven E. Ealick 2 1 Institute of Biochemistry, Graz University ... this area has been the subject of numerous review articles that have attempted to fathom and rationalize the capabilities of the flavin cofactor [2 6–3 2]....

Ngày tải lên: 28/03/2014, 22:20

... FEBS REVIEW ARTICLE Flavogenomics – a genomic and structural view of flavin-dependent proteins Peter Macheroux 1,2 , Barbara Kappes 3 and Steven E. Ealick 2 1 Institute of Biochemistry, Graz University ... this area has been the subject of numerous review articles that have attempted to fathom and rationalize the capabilities of the flavin cofactor [2 6–3 2]....

Ngày tải lên: 28/03/2014, 22:21

... & Matsuzawa H (1998) Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis ... X-ray crystallography and structural stability of digestive lysozyme from cow stomach Yasuhiro Nonaka 1 , Daisuke Akieda 1 , Tomoyasu Aizawa 1 , Nobuhisa Watanabe 1,2 , Masakat...

Ngày tải lên: 23/03/2014, 04:21

... roles of these Hbs and on the molecular adaptation mechanisms of these aquatic organisms living under particular environmental conditions. Materials and methods All reagents were of analytical grade. ... conformational state also at low pH. On the contrary, the pH decrease induces similar structural changes, characteristics of ligand dissociation and R fi T transition, both in...

Ngày tải lên: 23/03/2014, 12:20

... functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 Naoko Uekama, Takio Sugita, Masashi Okada, Hitoshi Yagisawa and Satoru Tuzi Graduate ... cyto- skeletal organization, and membrane trafficking are known to be localized at membrane surfaces of the plasma membrane and organelles. For instance, extra- cellular signals...

Ngày tải lên: 30/03/2014, 10:20

... and run on 6% polyacrylamide sequencing gels: extension products were analyzed and quantified on a Fuji Bio-Imaging analyzer BAS-2500 using IMAGE GAUGE V3.3 software. Chromatin and protein–DNA ... used a chloroquine concentration of 60 lgÆmL )1 . Radioactivity scans and quantifications were performed using a Fuji Bio-Imaging analyzer BAS-2500 with IMAGE GAUGE V3.3 software. Anal...

Ngày tải lên: 19/02/2014, 12:20

... the algorithm of Hockenmaier and Steed- man (2005), and similar to GPSG’s slash-passing (Gazdar et al., 1985). These trace categories are appended to the category of the head node (and other arguments ... Buch. (2) a. Gibt Peter Maria das Buch? b. Gib Maria das Buch! (3) a. dass Peter Maria das Buch gi bt. b. das Buch, das Peter Maria gibt. Local Scrambling In the so-called “Mittel...

Ngày tải lên: 08/03/2014, 02:21

... 1.35-m M phosphatase substrate (SIGMA 104Ò, Sigma-Aldrich, Bornem, Belgium) solution was added and the absorbance was measured after 30 and 60 min at 405 nm on a SPECTRAmax PLUS 384 microplate reader system ... was recorded by sensorgrams allowing an association time of 300 s and a dissociation of 180 s under a constant flow rate of 20 lLÆmin )1 at 25 °C. The sensorgram profil...

Ngày tải lên: 08/03/2014, 08:20

... enzymes were used to separate individual domains: D1, MfeI and XhoI; D2, XhoI and AatII; D3, AatII and AvrII. PCR using Ex Taq HS polymerase (Taka- ra USA, Santa Ana, CA, USA) was performed to fill ... in adjacent active sites, essentially allowing only one of a set of interacting active sites to complete a catalytic cycle at a time. Further understanding of catalysis and t...

Ngày tải lên: 16/03/2014, 06:20

... purification, character- ization and structural analysis of charybdin, a novel 29-kDa type 1 ribosome-inactivating protein, from bulbs of the white variety of C. maritima agg. Results Charybdin was ... characterization and cry- stallization of charybdin. The yield of the purified pro- tein was 15 0–2 00 mg protein per 100 g of bulbs. Charybdin appeared as a single band w...

Ngày tải lên: 16/03/2014, 14:20