Báo cáo khoa học: Heme binding to the second, lower-afﬁnity

Báo cáo khoa học: Heme binding to the second, lower-afﬁnity site of the global iron regulator Irr from Rhizobium leguminosarum promotes oligomerization potx

... binding to the second, lower-afﬁnity site of the global iron regulator Irr from Rhizobium leguminosarum promotes oligomerization Gaye F. White 1,2 , Chloe Singleton 1 , Jonathan D. Todd 2 , Myles R. ... insights into the mecha- nisms and consequences of heme binding to Irr. In addition to the HxH motif, Irr binds heme at a second, lower-...

Ngày tải lên : 28/03/2014, 22:21

11
281
0

Báo cáo khoa học: Oxygen binding and its allosteric control in hemoglobin of the primitive branchiopod crustacean Triops cancriformis pdf

... out on the basis of the SSR (Fig. 7). The details of the contour maps vary somewhat with changing number of binding sites for protons and Mg 2+ , but the principal behavior is maintained. The lowest ... describe the data well. The result of this analysis is shown in Table 2 together with the results of the other models. The residual plots of all models are...

Ngày tải lên : 23/03/2014, 09:20

18
460
0

Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc

... Sudlow site I and corresponding to the warfarin -binding pocket) sites show low afﬁnity for FAs, whereas the FA1 (also named the heme site) and the FA2 (corresponding to the regulatory site of the ... at the heme- binding pocket (FA1). Indeed, ibuprofen binding to HSA heme has been reported to induce the hexa-coordination of the heme Fe atom [18,2...

Ngày tải lên : 06/03/2014, 01:20

9
489
0

Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc

... reduces the cytotoxic potential of S23906-1 towards KB-3-1 epidermoid carcinoma cells. The GSH-induced threefold reduction of the cytotoxicity of S23906-1 is attributed to the reduced formation of ... case, the GSH–benzoacronycine adducts reduce the cytotoxic action of the drug. It should be included in the second group of compounds for which the covalent bind...

Ngày tải lên : 20/02/2014, 11:20

12
701
0

Báo cáo khoa học: Nucleotide binding to human UMP-CMP kinase using ﬂuorescent derivatives ) a screening based on afﬁnity for the UMP-CMP binding site potx

... the phosphate group of dCMP. This prevents the transfer of the c-phosphate from ATP. The ﬂexibility of the acyclic part of the tested compounds may prevent these inter- actions. The size of the ... screening of new compounds. Results and Discussion Competitive ﬂuorescence experiments to determine the binding of natural substrates to the donor and the...

Ngày tải lên : 07/03/2014, 09:20

11
468
0

Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... Loss of the LBD abolished the ability of C-Vrp1p 364)817 to restore growth of vrp1D cells at elevated temperature. We next assessed the importance of the LBD for the ability of C-Vrp1p 364)817 to ... C-Vrp1p to membranes and the cortical actin cytoskeleton. Intriguingly, the LBD is sufﬁcient to restore endocytosis and growth at elevated temperature to Vrp1p-d...

Ngày tải lên : 18/02/2014, 16:20

23
679
0

Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx

... 2). The intensity of the circular dichroic band decreases upon addition of calcium ions because calcium displaces the dye bound to calcium -binding sites of the protein. Other proteins of this ... Isothermal titration calorimetry: (A) calcium -binding isotherm of bB2-crystallin. (B) Terbium binding isotherm of bA3-crystallin. The best ﬁt to four -site sequenti...

Ngày tải lên : 18/02/2014, 16:20

13
449
0

Báo cáo khoa học: Relation between domain evolution, speciﬁcity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot

... matches best the a-amylase from Streptomyces griseus, a representative of the a-amylases produced by streptomycetes. The positioning of a Nostoc CGTase in the assumed amylase group of the SBD tree ... to the sequences of a-amylases originating from the streptomycetes than the two Pseudo- monas maltotetraohydrolases (Psesa and Psest) (Fig. 3A). It is worth menti...

Ngày tải lên : 08/03/2014, 08:20

11
615
0

Báo cáo khóa học: Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery–Dreifuss muscular dystrophy pdf

... as binding to other proteins at the inner nuclear membrane. Two of emerin’s known binding partners are lamin A and barrier -to- autointegration factor (BAF) [4,5]. The 40-residue ÔLEM-domainÕ of ... with EcoRI and SalI, and inserted into the p-GBK vector (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the SalIandBamHI sites in the vector. The DNA sequences of a...

Ngày tải lên : 23/03/2014, 12:20

11
377
0

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... concentrations almost mirror each other, although there are quantita- tive differences in the changes of the period within the oscillatory domain: a 2.7-fold decrease (from the high- est to the lowest values) ... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the cri...

Ngày tải lên : 18/02/2014, 11:20

17
512
0