Báo cáo khoa học: Discriminating between the activities of human cathepsin G and chymase using fluorogenic substrates potx
Báo cáo khoa học: Discriminating between the activities of human cathepsin G and chymase using fluorogenic substrates potx
... No claim to original French government works Discriminating between the activities of human cathepsin G and chymase using fluorogenic substrates Brice Korkmaz 1,2 , Gwenhael Je ´ got 1,2 , Laurie ... G Chymase Ser40 Arg143 Arg41 Lys192 Lys192 Phe41 His57 Ile99 Ile99 Cys42 Ser40 His57 Arg143 Lys40 Cathepsin G Chymase S1’ S2’ S2’ A B S1’ Fig. 1. Structural d...
Ngày tải lên: 28/03/2014, 22:20
Báo cáo khoa học: Relationship between the structure of guanidines and N-hydroxyguanidines, their binding to inducible nitric oxide synthase (iNOS) and their iNOS-catalysed oxidation to NO pptx
... positionings of the N-hydroxy- guanidine moiety of ClPhNOHG and BuNOHG involving: (a) a salt bridge between a glutamate side chain and the two nonhydroxylated nitrogens of the N-hydroxyguanidine, and ... specificity of iNOS FEBS Journal 272 (2005) 3172–3183 ª 2005 FEBS 3175 with l-Arg, with the order l-Arg > homo-l- Arg TFBGua>PentylGua>BuGua>>FPhGua ...
Ngày tải lên: 16/03/2014, 22:20
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... 5¢-gattgccaggctgttgtctcctcccag-3¢, 5¢-GTTGATGTCAGCATTGGCATCAACCCA-3¢ and 5¢-GGGGTGTGTCGATGTCA-3¢ for His120Asn, His145Asn and Asn149Asp, respectively. The corresponding sense muta- genic oligonucleotide primers were 5¢-CTGGGAGGAGA CAACAGCCTGGCAATC-3¢ ... [40], using the QuickChange site-directed mutagenesis kit (Strata- gene, La Jolla, CA, USA). The antisense mutagenic oligo- nucl...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... 1742 α -FlagEGFP + α -Flag Δ KIND2-Flag v-KIND-Flag dendrite axon Δ KIND1-Flag Δ KIND1+2-Flag KIND1-Flag Δ RasN-Flag α -FlagEGFP + α -Flag KIND2-Flag Δ GEF-Flag A B Fig. 1. Domain structure of the ... mor- phology (Fig. 5A). Sholl analysis of the dendrite PD: GST-CD2 IB: -GFP Input IB: -GFP GST-CD2 (CBB stain) EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EG...
Ngày tải lên: 14/02/2014, 19:20
Báo cáo khoa học: Distinguishing between different pathways of bilayer disruption by the related antimicrobial peptides cecropin B, B1 and B3 pptx
... by SPR, and results were collected and analysed using the manufacturer’s software and protocols. A diagram of the construction of the biosensor surface is shown in Fig. 1. Binding of peptides to the ... l M ,and controls consisted of DSC analysis of each peptide in the absence of lipid and the liposomes without peptide. Preparation of GUVs GUVs were used...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase potx
... PGK*MgADP [9]), 1VJC (pig muscle PGK*MgATP [83]), 1HDI (pig muscle PGK*3PG*MgADP [5]), 1KF0 (pig muscle PGK*3PG* MgAMPPCP [80]), 13PK (T. brucei PGK*3PG*MgADP [79]), 1VPE (T. maritima PGK*3PG*MgAMPPNP ... by the two substrates against thermal unfolding The thermal stability of PGK was further increased in the ternary complexes, i.e. in the simultaneous presence of two substrate...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Relationships between the ethanol utilization (alc ) pathway and unrelated catabolic pathways in Aspergillus nidulans pptx
... plasmids harboring the facA and facB genes, Irene Garcia for the prnD probe and Joan Tilburn for the gabA cDNA clone. We are grateful to Sabine Fillinger Fig. 8. Formation of the physiological aldehyde ... panel) andupongrowthon D -galacturonic acid ( D -GAA*)(rightpanel). (B) Northern blot analysis of the response of the alc genes to addition of D -glyceraldehyde and...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khóa học: Interaction between the 2¢)5¢ oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1 potx
... p59 OASL; lanes 2 and 3 (WASH), GST–MBD1 beads; lane 4, pull-down ofp59OASLusingGST–MBD1beads;lanes 5and6 (WASH),GST beads; lane 7 (control), pull-down of p59 OASL using GST beads. The pGEH–GST–MBD1 construct ... a general RNA degradation, leading to the inhibition of viral protein synthesis [10]. In humans, the OAS gene family is composed of four genes located on chromosome 1...
Ngày tải lên: 30/03/2014, 13:20
Báo cáo khoa học: "Reading Between the Lines: Learning to Map High-level Instructions to Commands" ppt
... Winograd. 1972. Understanding Natural Lan- guage. Academic Press. Chen Yu and Dana H. Ballard. 2004. On the integra- tion of grounding language and learning objects. In Proceedings of AAAI, pages ... both the mapping between the text and the commands, and the un- derlying model of the environment. One partic- ularly noteworthy aspect of our solution is the in-...
Ngày tải lên: 30/03/2014, 21:20
Báo cáo khoa học: Insights into the design of a hybrid system between Anabaena ferredoxin-NADP+ reductase and bovine adrenodoxin pot
... 3A), suggesting that the formation of the optimal complex between the two proteins limits the ET process. When analysing the effect of ionic strength on the interaction between FNR rd and Adx ox (Fig. ... multitude of reactions in microorganisms, plants and animals. In the case of Anabaena, a photosynthetic nitrogen-fixing cyanobacte- rium, Fd is involved in the...
Ngày tải lên: 31/03/2014, 07:20