... perturbation of the subunit interactions and is a powerful tool for using to study cavities in proteins [24,25]. The coat protein of bacteriophage MS2 contains two tryptophan residues, thuspermitting ... over the range of appreciable values of F. The values of center of spectralmass of tryptophan were converted into degree of denatur-ation/dissociation (ap), according to the following equation:ap¼ðmpÀ ... quantities of protein utilizing theprinciple of protein–dye binding. Anal. Biochem. 72, 248–254.27. Paladini, A.A. & Weber, G. (1981) Pressure-induced reversibledissociation of enolase....