Báo cáo Y học: Expression of the aspartate/glutamate mitochondrial carriers aralar1 and citrin during development and in adult rat tissues docx
Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
... [10]. The YN11 strain was derived fromYPH499 strain, and YN45 strain was prepared by the use of YN11 and BJ5458 strains.Preparation of AACEVAPD1 –650RACE and 30RACE products of the respective ... to colonySouthern hybridization. The nucleotide sequences of plasmid DNAs were confirmed by restriction mapping and by DNA sequencing. In the cases of AACEVAPD3 and 6, one TAA codon in their ORFs, ... fractions. The resultsFig. 2. Constructs for expression of AACEVAPD1–6 in yeast. The whole reading frames of AACEVAPD1 –6 were inserted into the sitebetween the yeast glyceraldehyde 3-phosphate dehydrogenase(GraP...
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Báo cáo Y học: Expression of the aspartate/glutamate mitochondrial carriers aralar1 and citrin during development and in adult rat tissues docx
... kidney and heart, the precise tissue distribution of the two proteins in embryonic and adult tissues is largelyunknown. We investigated the pattern of expression of aralar1 and citrin in murine ... predom-inant in brain, heart and skeletal muscle, whereas citrin expression only predominates in kidney.Distribution of AGC isoforms in tissues from the adult rat In adult rat tissues, the distribution ... This explainswhy citrin deficiency in humans (type II citrullinemia) onlyaffects the liver and suggests that aralar1 may compensatefor the lack of citrin in other tissues. Keywords: aspartate/glutamate...
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Báo cáo Y học: Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo potx
... 3¢,3¢-diaminobenzidinehydrochloride c hromogen (Sigma). The speci®city of the method was tested by the omission of the primary antibody in the staining, and the use of p reimmune serum instead of the ... mitigates development of obesityby both, increasing energy expenditure and d ecreasing in situlipogenesis. In order to further analyse consequences of respiratory uncoupling in white fat, the eects ... HoraÂkovaÂ1, Ivo SyrovyÂ1, Saverio Cinti2 and Jan KopeckyÂ11Department of Adipose Tissue Biology and Center for Integrated Genomics, Institute of Physiology,Academy of Sciences of the Czech...
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Báo cáo Y học: Characterization of the 105-kDa molecular chaperone Identification, biochemical properties, and localization pptx
... protein (APG-1/OSP94) the same as HSP90 in rat testis and brain. Expression time of 105-kDa protein in rat brain In order to clarify the localization of the 105-kDa protein in the brain, the rat ... immuno-histochemical analysis, the protein was mainly detected in the cytoplasm of the nerve and glial cells of the rat brain.Although the 105-kDa protein was localized in all rat brainsegments, the expression ... may cross-react with the 105-kDa protein similar to HSP90 in the rat testis and brain.We have investigated the localization and developmentalinduction of the protein in the rat brain. In the...
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Báo cáo Y học: Expression of recombinant murine pregnancy-associated plasma protein-A (PAPP-A) and a novel variant (PAPP-Ai) with differential proteolytic activity pot
... expressed in several tissues. The level of mRNA in the murine placenta does notexceed the levels of other tissues analyzed. Furthermore, the IGFBP-4-proteolytic activity of murine pregnancy serum ... level of intact IGFBP-4 in murine pregnancy was caused by anincrease in synthesis along with increased proteolysis. The presence of PAPP-A mRNA in all murine tissues analyzed parallels the ubiquitous ... position of the insert of PAPP-Ai,within the proteolytic domain, lies in close proximity to the cysteine residue, which in human PAPP-A forms a disulfidebond with the proform of eosinophil major...
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Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
... omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification and Statistical analysis ... hPMS2 and GTBP/hMSH6 are very important in distinguishing and repairing misparing and slippage errors in DNA synthesis. The mismatch is detected by two complexes, the hMSH2-GTBP/hMSH6 and the ... that 5% of the cases of oral squamous cell carcinoma show diminished expression of hMSH2 protein. In the present study, we demonstrated decreased expression of hMSH2 protein in reticular and atrophic/erosive...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... aninternal control for mRNA integrity and equal loading. The levels of radioactivity incorporated into c-myc product werenormalized by comparison with the levels of radioactivityincorporated ... NAMI-A-mediated inhibition of ERK1/2 phosphorylation may be lying upstream of ERK1/2.Using a serine/threonine kinase SPA assay kit, the capability of NAMI-A to affect the ERK1/2 phosphoryla-tion activity ... activity reported in carcinoma cells suggest that its overexpression may be of critical relevance in the maintenance of tumor cell growth[3]. The finding that the ERK signaling pathway is involvedin...
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Báo cáo y học: "Use of the measure your medical outcome profile (MYMOP2) and W-BQ12 (Well-Being) outcomes measures to evaluate chiropractic treatment: an observational study"
... objective was to investigate the validity of the MyMOP2 instrument to detect a change in well-being of patients attending the RMIT chiroprac-tic teaching clinic. The mean baseline MYMOP2 profile score ... study.Patients were invited to participate in the study if theywere: over the age of 18 years; had no treatment fromany health professional for their complaint in the preced-ing four weeks; and suffered ... deliveredby student chiropractors in a clinical teaching facility. The W-BQ12 was also used as a tool to assess the validity of the well being component of the MyMOP2 against the validated W-BQ12 instrument...
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Báo cáo y học: "Impact of the Kenya post-election crisis on clinic attendance and medication adherence for HIV-infected children in western Kenya"
... University School of Medi-cine and Moi Teaching and Referral Hospital (Eldoret,Kenya) and the Institutional Review Board of the IndianaUniversity School of Medicine (Indianapolis, Indiana).Informed ... There was one boy who was being taken care of by the uncle. They stay in Langas. Langas was, let me say, it was the heat of the violence there. This boy is on second linemedication, and at the ... sampling to iden-tify key informants, including physicians, nurses, and clinical officers, based on their locations and roles during the conflict. A trained facilitator conducted 9 interviewsusing...
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Báo cáo y học: "Evaluation of the Prevalence of Concomitant Idiopathic Cyclic Edema and Cellulit"
... on a clinical history and fluid retention throughout the day, in particular difficulty in removing rings on waking in the morning which improves later in the day. All patients with cyclic edema ... edema. The diagnosis of idiopathic cyclic edema was clinical based on the clinical history and fluid reten-tion throughout the day and, in particular, difficulty to remove rings on waking in the ... the morning which im-proves later in the day. Some patients reported facial edema early in the morning and swelling of the legs at the end of the day. Participants were asked to weigh themselves...
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