... [10]. The YN11 strain was derived from YPH499 strain, and YN45 strain was prepared by the use of YN11 and BJ5458 strains. Preparation of AACEVAPD1 –6 5 0 RACE and 3 0 RACE products of the respective ... to colony Southern hybridization. The nucleotide sequences of plasmid DNAs were confirmed by restriction mapping and by DNA sequencing. In the cases of AACEVAPD3...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Expression of the aspartate/glutamate mitochondrial carriers aralar1 and citrin during development and in adult rat tissues docx
... kidney and heart, the precise tissue distribution of the two proteins in embryonic and adult tissues is largely unknown. We investigated the pattern of expression of aralar1 and citrin in murine ... predom- inant in brain, heart and skeletal muscle, whereas citrin expression only predominates in kidney. Distribution of AGC isoforms in tissues fr...
Ngày tải lên: 24/03/2014, 04:21
... 3¢,3¢-diaminobenzidine hydrochloride c hromogen (Sigma). The speci®city of the method was tested by the omission of the primary antibody in the staining, and the use of p reimmune serum instead of the ... mitigates development of obesity by both, increasing energy expenditure and d ecreasing in situ lipogenesis. In order to further analyse consequences of...
Ngày tải lên: 31/03/2014, 15:20
Báo cáo Y học: Characterization of the 105-kDa molecular chaperone Identification, biochemical properties, and localization pptx
... protein (APG-1/OSP94) the same as HSP90 in rat testis and brain. Expression time of 105-kDa protein in rat brain In order to clarify the localization of the 105-kDa protein in the brain, the rat ... immuno- histochemical analysis, the protein was mainly detected in the cytoplasm of the nerve and glial cells of the rat brain. Although the 105...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Expression of recombinant murine pregnancy-associated plasma protein-A (PAPP-A) and a novel variant (PAPP-Ai) with differential proteolytic activity pot
... expressed in several tissues. The level of mRNA in the murine placenta does not exceed the levels of other tissues analyzed. Furthermore, the IGFBP-4-proteolytic activity of murine pregnancy serum ... level of intact IGFBP-4 in murine pregnancy was caused by an increase in synthesis along with increased proteolysis. The presence of PAPP-A mRNA in all murine t...
Ngày tải lên: 31/03/2014, 21:21
Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
... omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification and Statistical analysis ... hPMS2 and GTBP/hMSH6 are very important in distinguishing and repairing misparing and slippage errors in DNA synthesis. The mismatch is detected by two complexe...
Ngày tải lên: 03/11/2012, 09:54
Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... an internal control for mRNA integrity and equal loading. The levels of radioactivity incorporated into c-myc product were normalized by comparison with the levels of radioactivity incorporated ... NAMI-A-mediated inhibition of ERK1/2 phosphorylation may be lying upstream of ERK1/2. Using a serine/threonine kinase SPA assay kit, the capability of NAMI-A to affect the ERK1/...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo y học: "Use of the measure your medical outcome profile (MYMOP2) and W-BQ12 (Well-Being) outcomes measures to evaluate chiropractic treatment: an observational study"
... objective was to investigate the validity of the MyMOP2 instrument to detect a change in well-being of patients attending the RMIT chiroprac- tic teaching clinic. The mean baseline MYMOP2 profile score ... study. Patients were invited to participate in the study if they were: over the age of 18 years; had no treatment from any health professional for their complaint in...
Ngày tải lên: 25/10/2012, 10:06
Báo cáo y học: "Impact of the Kenya post-election crisis on clinic attendance and medication adherence for HIV-infected children in western Kenya"
... University School of Medi- cine and Moi Teaching and Referral Hospital (Eldoret, Kenya) and the Institutional Review Board of the Indiana University School of Medicine (Indianapolis, Indiana). Informed ... There was one boy who was being taken care of by the uncle. They stay in Langas. Langas was, let me say, it was the heat of the violence there. This boy is on se...
Ngày tải lên: 25/10/2012, 10:31
Báo cáo y học: "Evaluation of the Prevalence of Concomitant Idiopathic Cyclic Edema and Cellulit"
... on a clinical history and fluid retention throughout the day, in particular difficulty in removing rings on waking in the morning which improves later in the day. All patients with cyclic edema ... edema. The diagnosis of idiopathic cyclic edema was clinical based on the clinical history and fluid reten- tion throughout the day and, in particular, difficulty...
Ngày tải lên: 25/10/2012, 10:51