Báo cáo Y học: Herbaspirillum seropedicae signal transduction protein PII is structurally similar to the enteric GlnK potx
Báo cáo Y học: Herbaspirillum seropedicae signal transduction protein PII is structurally similar to the enteric GlnK potx
... (A)AlignmentsoftheHsPIIwithEcPIIand HsPII with EcGlnK amino-acidsequences. The identity (73%) and similarity(86%) of HsPII to EcPII is higher than HsPII to EcGlnK (67% and 76%, respectively).Secondary ... to the EcGlnK protein (Fig. 1B). The structural relatedness ofHsPII and EcGlnK in their C-terminal regions may explainwhy HsPII is functionally similar to the KpGlnK andEcGlnK proteins. HsPII ... does EcGlnK, EcGlnk-ATP and PII- ATP(Fig. 1B). Although the identity (73%) and similarity (83%)of HsPII to EcPII is higher than that of HsPII to EcGlnK(67% and 76%, respectively), HsPII is structurally...
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Báo cáo Y học: Double-stranded RNA-activated protein kinase interacts with apoptosis signal-regulating kinase 1 Implications for apoptosis signaling pathways docx
... deleted of the PKRgene have been shown to resist apoptosis in response to dsRNA, tumor necrosis factor-a (TNF-a) or lipopolysac-charide (LPS) [13]. PKR has been shown to play some rolein the activation ... reported[24]. The signal for PKR-KR was predominantly localizedin the cytoplasm, whereas that for ASK1 distributeddiffusely with relatively intense staining at the periphery of the cells (Fig. ... mutant of ASK1carrying a point mutation of K to M at position 709 (ASK-KM) were kindly provided by H. Ichijo (Laboratory of CellSignaling, Tokyo Medical and Dental University). The plasmid encoding...
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Báo cáo y học: " Post-traumatic glioma: Report of one case and review of the literature"
... hematoma in the right temporal lobe at the site of the subsequent glioblastoma. The intracranial he-matoma was treated conservatively at that time. After the diagnosis of the glioblastoma ... injury. 2. The below must be severe enough to cause brain contusion and a secondary reparative process. 3. The location of the impact and the tumor should correspond exactly one to the other. ... impact and the occur-rence of the tumor corresponded exactly one to the other; and (4) there was a more than one year interval between trauma and the appearance of the tumor. As regards the pathogenesis...
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Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture"
... To test the hypo-thesis that B19-associated liver pathology is related to the direct toxicity of persistent B19 NS1 in hepatocytes of previously infected individuals, we examined the possible ... lead us to examine the potential role of B19 NS1 in direct hepatocyte cytotoxicity. Our laboratory pre-viously demonstrated that B19 NS1 caused apoptosis in liver-derived cells (34, 35). The above ... (Becton-Dickson), and statistical analysis completed by FlowJo software (Tree Star, Inc, Ash-land, OR). Cytotoxicity Assay. Trypan blue dye exclusion was used to determine cell viability....
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Báo cáo y học: " Role of Dietary Soy Protein in Obesity"
... fat by partly inhibiting hepatic fatty acid synthesis in the liver. ACC, the rate-limiting en-zyme that catalyzes the carboxylation of acetyl-Co A to form malonyl-CoA, is the pivotal enzyme ... Genetically obese mice Soy protein isolate and hydrolysate vs milk whey protein isolate and hydrolysate 2 wks Decreased BW and perirenal fat 39 Obese KK-Ay mice Soy protein isolate vs casein protein, ... roasted soybeans, soy flour, and textured soy protein provide the highest concentrations of isoflavones, 0.1-5 mg total isoflavones/g soy protein [35]. Isolated soy protein and other soy protein...
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Tài liệu Báo cáo Y học: Biphasic reductive unfolding of ribonuclease A is temperature dependent pdf
... [10], dynamic analysis of the different processes is necessary to evaluate the different pathways. These studieswill also clarify the key factor(s) controlling the processesand clarify the unfolding ... image analysis,which is established by us recently [4,11], was used to analyze the unfolding dynamics and also was introducedinto the analysis of the thermal transition study by CD and1HNMR.MATERIALS ... properties. The close -to- unfolding state at 313 K of the three-disulfide intermediates may drive the slow process to the unfolded state. The selectivity mechanism maytherefore arise from the different thermodynamic...
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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... by solid-phase synthesis byAnsynth Service B.V. (the Netherlands) using the Fmoc/tert-butyl-based methodology with Rink resin as the solidsupport. The peptides were synthesized manually. The ... in the venom of scorpionsbelonging to the same family, independent of the continentand region where they live. In this study, the biologicalactivity of parabutoporin and opistoporin 1 is comparedwith ... only related to the defensemechanism of higher organisms, but they can also be usedby bacteria as a part of their pathogenicity (e.g. aerolysinfrom the bacterium Aeromonas hydrophila [28]).The...
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
... ofstorage starch synthesis show that amylopectin and amylosesynthesis are partly disconnected and that amylose synthesispersists when the rate of polysaccharide and amylopectinsynthesis become ... amylosesynthesis accounts for most polysaccharide synthesis. Atthis stage the rate of amylopectin synthesis has becomeminimal and it is difficult to say if the residualamylopectin synthesis ... foramylose synthesis but lacks the amylopectin synthesismachinery. This system consists of intact starch granulespurified from Chlamydomonas strains under physiologicalconditions where the synthesis...
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Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
... phosphorylationPhosphorylation of Ser79 is known to inactivate ACC bydecreasing the Vmax[18,23]. In vitro,thissiteisphospho-rylated by AMPK and dephosphorylated by GAPP.Immunoblotting hepatocyte ... anactivation of mTOR by dephosphorylation. Taken together,these data support the hypothesis that two phosphatases areinvolved, one upstream of mTOR, the activation of which is required for the activation ... in the control of protein synthesis and is activated in response to hormones, mitogens and nutrients (reviewed in [1–3]). Itphosphorylates the 40S ribosomal protein S6, which is involved in the...
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Tài liệu Báo cáo Y học: Transactivation domains are not functionally conserved between vertebrate and invertebrate serum response factors potx
... The migration of the fusion proteins containing the myc epitope: human (HsSRF-myc), D. melanogaster (DmSRF-myc) and A. fran-ciscana (AfSRF-myc) SRFs, is indicated to the right. The migration of ... may also compete with inhibitory moleculesbound to the CArG box or to SRF itself, either bound to DNA or in solution. Several inhibitory factors that bind to SRF or compete with SRF-binding to ... activatingcofactors. Alternatively, inhibition could be explained bydiluting of these cofactors by an excess of nonDNA-boundSRF. This would be the more likely mechanism of the inhibition observed by co-expression...
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