Báo cáo Y học: Pyruvate decarboxylase from Kluyveromyces lactis An enzyme with an extraordinary substrate activation behaviour pptx
... decarboxylase from brewer’s yeast; KlPDC, pyruvate decarboxylase from Kluyveromyces lactis; PsPDC, pyruvate decarboxylase from Pisum sativum;ScPDC, pyruvate decarboxylase from Saccharomyces cerevisiae;ZmPDC, pyruvate ... Pyruvate decarboxylase from Kluyveromyces lactis An enzyme with an extraordinary substrate activation behaviour Florian...
Ngày tải lên: 24/03/2014, 04:21
... Piotrowski 2 , Steffen Reinbothe 2, * and Gerhard Link 1 1 Plant Cell Physiology & Molecular Biology and 2 Plant Physiology, University of Bochum, Germany The plastid transcription kinase (PTK), a ... were separated by SDS/PAGE and transferred to nitrocellulose membranes. They were then probed with purified cpCK2a primary antibody at 4 °C for 12 h, followed by incubation with anti-(rab...
Ngày tải lên: 24/03/2014, 04:21
... sub- strate activation process of Kluyveromyces lactis pyruvate decarboxylase. Abbreviations KlPDC, pyruvate decarboxylase from Kluyveromyces lactis; PDC, pyruvate decarboxylase; ScPDC, pyruvate decarboxylase ... July 2006) doi:10.1111/j.1742-4658.2006.05415.x The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis has been determined to 2...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo y học: "Does switching from oral extended-release methylphenidate to the methylphenidate transdermal system affect health-related quality-of-life and medication satisfaction for children with attention-deficit/hyperactivity disorder&
... Feedback from teachers and parents about my child with ADHD has been generally positive • My child was able to deal well with everyday hassles and frustrations with friends and siblings • My child ... KE, Gnagy EM, Hoffman MT, Onyango AN, Fabiano GA, Lopez-Williams A, Wymbs BT, Caserta D, Chronis AM, Burrows-Maclean L, Morse G: A dose- ranging study of a methylphenidate transdermal s...
Ngày tải lên: 25/10/2012, 10:06
Tài liệu Báo cáo Y học: Caged O2 Reaction of cytochrome bo3 oxidase with photochemically released dioxygen from a cobalt peroxo complex doc
... (1994) Energy transduction by cytochrome complexes in mitochondrial and bacterial respiration: the enzymology of coupling electron transfer reactions to transmembrane proton translocation. Annu. Rev. ... in FT-IR type sample cuvette with 500-lm thickness. Yields were determined from the absorbance changes at 293 nm. Fig. 4. Reaction of cytochrome bo 3 fully reduced by the substrate dur-...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Ornithine decarboxylase-antizyme is rapidly degraded through a mechanism that requires functional ubiquitin-dependent proteolytic activity pot
... binds to antizyme with higher affinity than ODC thus it may release active ODC from the inactive antizyme–ODC heterodimer [20]. While it is clear that interaction with antizyme is absolutely required ... how interaction with antizyme inhibitor [19,20,41–43] affects antizyme degradation. The observation that antizyme may be degraded inde- pendently of ODC raised the possibility t hat an...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo Y học: Fluorescent analogs of UDP-glucose and their use in characterizing substrate binding by toxin A from Clostridium difficile pdf
... commonly used to probe the chemistry of ATP-dependent enzymes. The reaction of excess methylisa- toic anhydride with UDP-Glc in aqueous solution yields primarily the 2¢ and 3¢ isomers of methylanthraniloyl-UDP- Glc ... available methylanthraniloyl (mant) derivatives of ATP and GTP commonly used in mechanistic studies of ATPases and GTPases. Here, we report the synthesis, purification and cha...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo Y học: Cytochrome c from a thermophilic bacterium has provided insights into the mechanisms of protein maturation, folding, and stability potx
... J., Yoshida, T., Yamazaki, T. & Sambongi, Y. , Yu, Y. , Igarashi, Y. , Kodama, T., Yamazaki, K., Kyogoku, Y. & Kobayashi, Y. (1998) Solution structure of thermostable cyto- chrome c-552 from ... quintuple mutant, and HT c-552. The heat capacity curves obtained by differential scanning calorimetry of wild-type PA c-551, the quintuple mutant, and HT c-552 at pH 5.0 with 1.5 M g...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
... endo-type hydrolysis. Consequently, this glucanase should be classified as an exo-glucohydrolase (Fig. 2). The enzyme displayed only very low activity against 1,3-1,4-b-glucans, lichenan, and barley ... exo-1,3-b-glucanases of fungal origin typically display optimal enzymatic activity in the pH range between 4 and 6 [19,23,43]. Similarly the enzyme from T. viride AZ36 displayed optimal...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo y học: "Pre-hospital intubation by anaesthesiologists in patients with severe trauma: an audit of a Norwegian helicopter emergency medical service"
... patients with severe trauma was much better than those reported from many non-physician-staffed EMS systems [4,5] and similar to other physician-manned EMS systems [17,18]. The safety of pre-hospital ... pre-hospital emergency medical systems (EMS), including the national helicopter emergency system (HEMS) in Norway, have used anaesthesiologists as pre- hospital emergency physicians for m...
Ngày tải lên: 25/10/2012, 09:56