... the detection of E2F6 mRNA with primers SG122 and SG24, the following conditions were used: 1 cycle: 30 min, 50 °C; 1 cycle: 2 min, 94 °C; 35 cycles: 30 s, 94 °C, 30 s, 55 °C;1min,72°C, followed by 1 cycle ... (reviewed in [1] ). Consequently, deregulation of E2F can promote tumorigenic transformation (reviewed in [2]). In normal cells, E2F activity is regulated by the binding to pRB, the product of the retinoblastoma ... Expression of E2F6 and E2F6b during the cell cycle. Mouse primary fibroblasts were brought to quiescence byincubationfor48hinserumfreemedium,andthenreleasedintothecell cycle by the addition of 10 %...