Báo cáo Y học: Molecular interactions between nuclear factor kB (NF-kB) transcription factors and a PNA– DNA chimera mimicking NF-kB binding sites doc

... of NF -kB DNA DNA, PNA–PNA and PDP–PDP and GATA-1 and NF-IL2 DNA DNA hybrids on the interaction between crude nuclear extracts from B-lymphoid Raij cells and 32 P-labelled HIV-1 NF -kB DNA DNA target ... prediction of molecular interactions between chimeras and NF -kB nuclear proteins were investigated by molecular dynamics simulations, and interactions be...

Ngày tải lên: 24/03/2014, 04:21

... of transcriptional factors on the pathology of rheumatoid arthritis (RA). Nuclear factor- jB The nuclear factor- jB (NF-jB) proteins are a family of ubiquitously expressed transcription factors that play an ... MINIREVIEW Molecular aspects of rheumatoid arthritis: role of transcription factors Hiroshi Okamoto 1 , Thomas P. Cujec 2 , Hisashi Yamanaka 1 and Naoyuki Kamatan...

Ngày tải lên: 18/02/2014, 18:20

... IRES-mediated translation and polyprotein processing as well as membranous web formation and RNA replication, illustrated here as separate steps for simplicity, may occur in a tightly coupled manner. ... genotype and other factors, this strategy results in a sustained virologic response in 50-80% of patients [2-5]. However, many patients do not qualify for or do not tolerate sta...

Ngày tải lên: 02/11/2012, 10:00

... steady-state rates in presence of Ch4S were significantly different from those in their absence (one-way analysis of variance and Tukey multiple comparison test). One-way analysis of variance also ... endopeptidase elastase-2 from human polymorphonuclear leu- kocytes is associated with physiological remodeling and pathological deg- radation of the extracellular matrix. Glycosaminoglycans b...

Ngày tải lên: 16/02/2014, 14:20

... Chile; 2 Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada Escherichia coli phosphoenolpyruvate (PEP) carboxykinase catalyzes the decarboxylation of oxaloacetate and ... spectroscopy. Escherichia coli phosphoenolpyruvate carboxykinase [PEP carboxykinase; ATP:oxaloacetate carboxylase (trans-phos- phorylating) EC 4.1.1.49] catalyzes the reversible deca...

Ngày tải lên: 21/02/2014, 01:21

... long- and short-chain fatty acid substrates To study the functional impact of the LPL C-terminal domain on catalytic activity, wild-type and mutant proteins R40 5A, K41 3A, K41 3A/ K41 4A, K41 4A, and ... nm was determined at an excitation wavelength of 386 nm. Oleic acid was used as a standard for free fatty acid. Esterase activity was measured using p-nitrophenyl butyrate as a sub...

Ngày tải lên: 21/02/2014, 15:20

... 5) showed that Rab31, Rab32 and Rab1 1A were equally enriched in the granule/mitochondrion and membrane fractions and that no R ab31 or R ab32, and only a s mall amount of Rab1 1A, was present in ... from a platelet Marathon TM cDNA library, using as PCR primers, 5¢-TAGGATCCGCGATACGGGAGC- TCAAAG-3¢ (P31-1) and 5 ¢-ATCTCGAGGATGTGGG- Fig. 1. Nucleotide and deduced amino-acid s...

Ngày tải lên: 08/03/2014, 16:20

... glabra GgbAS1 b-amyrin synthase [6], was prepared by PCR using GgbAS1 as a template, Taq DNA polymerase (Takara Shuzo, Kyoto, Japan), the primers 5 0 -GAAGCATA TCCACTATGAAGATGA-3 0 and 5 0 -TGAATACTCCCGTG ATTTCCTGTTG-3 0 , ... Noboru Hiraoka 2 , Yasumasa Ikeshiro 2 , Kazufumi Yazaki 3 , Shigeo Tanaka 4 , Tetsuo Kushiro 5 , Masaaki Shibuya 5 and Yutaka Ebizuka 5 1 Gifu Pharmaceutical U...

Ngày tải lên: 08/03/2014, 23:20

... EMSA analyses, aside from the shifted band that contained USF1 and USF2, we observed other shifted bands (X and Y) . As bands X and Y were only partially inhibited by 500-fold excess of unlabelled ... for binding factors in nuclear extracts from SKOV3 (an ovarian cancer cell line) and MDA453 (a breast cancer cell line) (Fig. 5, lanes 2 and 7) for EMSA, we detected several...

Ngày tải lên: 08/03/2014, 23:20

... HRP-conjugated secondary Ig [anti-(mouse IgG) Ig or anti-(sheep IgG) Ig; Sigma Chemical Co.] and the ECL system (Amersham-Pharmacia). Enzyme assays and trehalase activation Trehalase activity was assayed ... was calibrated using vitamin B 12 (1.3 kDa), cytochrome c (12.4 kDa), carbonic anhydrase (29 kDa), ovalbumin (43 kDa), BSA (66 kDa), yeast alcohol dehydrogenase (150 kDa), b-amylase (...

Ngày tải lên: 08/03/2014, 23:20