Báo cáo Y học: Engineering and mechanistic studies of the Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS pot
Báo cáo Y học: Engineering and mechanistic studies of the Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS pot
... wereassayed for FAE1 KCS activity. All of the activity wasFig. 2. Hydropathy analysis of FAE1 KCS. (A) Hydropathy plot of FAE1 KCS indicating the presence of several hydrophobic regions. The position ... inactiveenzyme.Taken together, the analysis of the decarboxylationactivity and characterization of the mutants of the putativecatalytic triad strongly support the hypothesis that the membrane-bound FAE1 KCS shares ... Engineering and mechanistic studies of the Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS Mahin Ghanevati and Jan G. JaworskiDepartment of Chemistry and Biochemistry, Miami University,...
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Báo cáo Y học: Unfolding and refolding studies of frutalin, a tetrameric D-galactose binding lectin doc
... fluorescence emission of the protein at any concentration o f the denaturant is given by the sum of the contributions by the two states asaðobsÞ¼ aNfNþ aUfUð3Þwhere aN and aUare the maximum ... parallel b sheet, and b turns) and 16% of other contributions for both the native and refolded forms, with a rmsd of 1%. The deconvo lution of Mfrutalin showed 13% of a helix and 12% of b components(antiparallel ... and 348 nm for Ufrutalin, and Rfrutalin was closely similar to Nfrutalin for both kemissmax and intensity. The kemissmax of Mfrutalin was 353 nm and the intensity was similar to that of the...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... However, the catalytic effi-ciency (kcat⁄ Km) of PRTFDC1 towards hypoxanthine and guanine wasonly 0.26% and 0.09%, respectively, of that of HPRT. This low activitycould be explained by the fact ... 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remainsunclear and it is uncertain whether it has the capacityto compensate for a deficiency ... Val143 and Phe194. N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms,whereas the side chain of Lys173 interacts with bothO6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen...
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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
... parallel the effect of the absence of other homologous regulatory subunits of oxygenase complexes, like T4MOD [33] and componentB of methane monooxygenases [38,39]. In these cases the absence of the ... complexes involved in the oxygenation of aromatic compounds [4]. On the basis of homology studies of the coding gene sequence [4] it has beenhypothesized that the gene products of the cluster form ... on the expected molecular size of the polypep-tides, all the proteins of interest were present in the solublefraction of the induced cell in the case of the expression of pET22b(+)/touBEA and...
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Báo cáo Y học: Structural and serological relatedness of the O-antigens of Proteus penneri 1 and 4 from a novel Proteus serogroup O72 pptx
... Nikolay P. Arbatsky2,Alexander S. Shashkov2 and Yuriy A. Knirel21Department of General Microbiology, Institute of Microbiology and Immunology, University of èodzÂ, Poland;2N. D. ZelinskyInstitute ... judged by the ratio of the integral intensities of the signals in the major and minor series, the average degree of glycosylation with GlcIIis 75%.Comparison of the 13C NMR spectra of the initial ... species.These data ®tted well with a marked similarity of the structures 1 and 2 of the O-speci®c polysaccharides of the P. penneri 1 and 4 LPS, respectively. The two O-antigensshare the major...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... across the yeastcell cycle. They rapidly decrease w ith the entry of yeast cellsinto S phase and increase again during t he following G1phase with the synthesis of new Cdc6p.In contrast, the ... yeastCdc6p is phosphorylated and then rapidly destroyed b y ubiquitin-mediated protein degradation. The regulateddestruction of Cdc6p effectively prevents the binding of Mcm proteins, and therefore ... at the beginning (0–2 h afterthymidine-block) and in the middle of S phase (4–6 h), aswell as at the e nd of mitosis and during the early G1 phase(12–14 h) of the next cycle (Fig. 2A).Cytoplasmic...
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Báo cáo khoa học: Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide docx
... Kamysz1 and Wojciech Kamysz21 Faculty of Chemistry, University of Gdan´sk, Poland2 Faculty of Pharmacy, Medical University of Gdan´sk, PolandIntroduction The search for new drugs and ... Gly3–Arg8 and Cys14–Gly16 regions of IB-367 and in the Gly2–Cys6 and Val12–Val14 regions of BM-1 indicates a significantpopulation of conformers with dihedral angles in the b-strand region of ... tachyplesin I and polyphemusin I share two highly hydrophilic and positively charged poles in the N- and C-terminalregions and in the turn. Androctonin also has a highlyhydrophilic and positively charged...
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Báo cáo Y học: Engineering and use of 32P-labeled human recombinant interleukin-11 for receptor binding studies docx
... the presence of twofold dilutions of IL-11(2 lgÁmL)1). After 7 days of cultu re, the number o fsurviving cells was determined by a c olorimetric assay of hexosaminidase. I n this assay, the ... to the presence within the Flag tag and t he ph osphorylation s ite o f numerous charged residues(1Glu, 5Asp, 2Arg and 2Ly s) (Fig. 1).Biological activity of FPDIL-11 The availability of the ... the regulation of production of pro-in¯ammatory cytokinessuch as TNF-a,IL-1b,IFN-c and I L-12 by monocytes[55,56], and that the IL-11 receptor analysis on human celllines by ¯ow cytometry using monoclonal...
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Báo cáo y học: "A prospective observational study of the relationship of critical illness associated hyperglycaemia in medical ICU patients and subsequent development of type 2 diabetes"
... fortendency to hyperglycaemia that are the root of hospitalacquired hyperglycaemia and in the long term lead todevelopment of diabetes.Although the incidence of hospital acquired hypergly-caemia ... indexes.During the five years of follow-up, 102 (15.2%) patientsin the normoglycaemia group and 66 (18.3%) patients in the hyperglycaemia group died. There were 154 patientsin the normoglycaemia group and ... 0.05. The study was approved by the Ethics Committee of the University Hospital Centre. All patients included in the study signed an informed consent form. The study wasnot funded or supported by...
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Báo cáo y học: " Derivation and preliminary validation of an administrative claims-based algorithm for the effectiveness of medications for rheumatoid arthritis"
... Despite these strengths, we acknowledge that the potentially limited generalizability of patterns of care in the VHA system and possible dissimilarity in the RA patients it treats compared to other ... sensitivity and specificity, unlike PPV and NPV, should be less dependent on the prevalence in the population, and more reflective of the test itself, lessening the impact of any unique features of ... The extent of bias resulting from misclassification of our algorithm is described in Table 6. Varying a hypothetical response rate as measured by the algorithm from 30 and 60%, the amount of...
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Từ khóa: báo cáo y họcbáo cáo y học cổ truyềnbáo cáo khoa học y họcbáo cáo y tế học đườngmẫu báo cáo y tế học đườngbáo cáo y tế học đường cuối nămBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ