Báo cáo Y học: Engineering and mechanistic studies of the Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS pot

... were assayed for FAE1 KCS activity. All of the activity was Fig. 2. Hydropathy analysis of FAE1 KCS. (A) Hydropathy plot of FAE1 KCS indicating the presence of several hydrophobic regions. The position ... inactive enzyme. Taken together, the analysis of the decarboxylation activity and characterization of the mutants of the putative catalytic triad st...

Ngày tải lên: 24/03/2014, 03:21

... fluorescence emission of the protein at any concentration o f the denaturant is given by the sum of the contributions by the two states as a ðobsÞ ¼ a N f N þ a U f U ð3Þ where a N and a U are the maximum ... parallel b sheet, and b turns) and 16% of other contributions for both the native and refolded forms, with a rmsd of 1%. The deconvo lution of Mfrutalin...

Ngày tải lên: 17/03/2014, 23:20

... However, the catalytic effi- ciency (k cat ⁄ K m ) of PRTFDC1 towards hypoxanthine and guanine was only 0.26% and 0.09%, respectively, of that of HPRT. This low activity could be explained by the fact ... 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remains unclear and it is uncertain whether it has the cap...

Ngày tải lên: 15/02/2014, 01:20

... parallel the effect of the absence of other homologous regulatory subunits of oxygenase complexes, like T4MOD [33] and component B of methane monooxygenases [38,39]. In these cases the absence of the ... complexes involved in the oxygenation of aromatic compounds [4]. On the basis of homology studies of the coding gene sequence [4] it has been hypothesized th...

Ngày tải lên: 17/03/2014, 10:20

... Nikolay P. Arbatsky 2 , Alexander S. Shashkov 2 and Yuriy A. Knirel 2 1 Department of General Microbiology, Institute of Microbiology and Immunology, University of èodz  , Poland; 2 N. D. Zelinsky Institute ... judged by the ratio of the integral intensities of the signals in the major and minor series, the average degree of glycosylation with Glc II is  75%. Com...

Ngày tải lên: 17/03/2014, 17:20

... across the yeast cell cycle. They rapidly decrease w ith the entry of yeast cells into S phase and increase again during t he following G1 phase with the synthesis of new Cdc6p. In contrast, the ... yeast Cdc6p is phosphorylated and then rapidly destroyed b y ubiquitin-mediated protein degradation. The regulated destruction of Cdc6p effectively prevents the binding of...

Ngày tải lên: 17/03/2014, 23:20

... Kamysz 1 and Wojciech Kamysz 2 1 Faculty of Chemistry, University of Gdan ´ sk, Poland 2 Faculty of Pharmacy, Medical University of Gdan ´ sk, Poland Introduction The search for new drugs and ... Gly3–Arg8 and Cys14– Gly16 regions of IB-367 and in the Gly2–Cys6 and Val12–Val14 regions of BM-1 indicates a significant population of conformers with dihedral angles in...

Ngày tải lên: 22/03/2014, 21:20

... the presence of twofold dilutions of IL-11 (2 lgÁmL )1 ). After 7 days of cultu re, the number o f surviving cells was determined by a c olorimetric assay of hexosaminidase. I n this assay, the ... to the presence within the Flag tag and t he ph osphorylation s ite o f numerous charged residues (1Glu, 5Asp, 2Arg and 2Ly s) (Fig. 1). Biological activity of FPDIL-11 The...

Ngày tải lên: 24/03/2014, 00:21

... for tendency to hyperglycaemia that are the root of hospital acquired hyperglycaemia and in the long term lead to development of diabetes. Although the incidence of hospital acquired hypergly- caemia ... indexes. During the five years of follow-up, 102 (15.2%) patients in the normoglycaemia group and 66 (18.3%) patients in the hyperglycaemia group died. There were 154...

Ngày tải lên: 25/10/2012, 10:02

... Despite these strengths, we acknowledge that the potentially limited generalizability of patterns of care in the VHA system and possible dissimilarity in the RA patients it treats compared to other ... sensitivity and specificity, unlike PPV and NPV, should be less dependent on the prevalence in the population, and more reflective of the test itself, lessening th...

Ngày tải lên: 25/10/2012, 10:45