Báo cáo Y học: SF2/ASF protein inhibits camptothecin-induced DNA cleavage by human topoisomerase I potx

... relaxation catalysed by topoisomerase I. Keywords: topoisomerase I; SF2/ASF; camptothecin. Eukaryotic topoisomerase I (topo I) catalyses DNA relax- ation and plays a key role in DNA replication, transcription and ... incubated for 30 min at 30 °C. Lanes: 1, HindIII; 2, HindIII + htopo I; 3, HindIII + SF2/ASF; 4, HindIII + htopo I + SF2/ASF; 5, RsaI; 6, RsaI + htopo I;...

Ngày tải lên: 24/03/2014, 03:21

... 269)35 phosphorylation levels in the IFN cotreated cells. Based on these experiments it is unlikely that IFN inhibits cell cycle activation by inhibiting the PtdIns3K pathway, however, additional experiments ... Mannerheimintie 166, Helsinki, Finland It has previously been shown that IFN-a is a potent inhibitor of IL-2 indu ced proliferation in p rimary T-lymphocytes, by selectively a b...

Ngày tải lên: 31/03/2014, 15:20

... Ranter 1 1 Laboratory for Analytical Chemistry and Medicinal Physicochemistry, Faculty of Pharmaceutical Sciences, K.U. Leuven, Leuven, Belgium; 2 Laboratory of Physiological Chemistry, Christian de Duve Institute ... Mg 2+ .The sixfold coordinated Mg 2+ ion present in the a ctive site of HPSP under normal physiological conditions, was replaced by a Ca 2+ ion by using a crystallization co...

Ngày tải lên: 16/03/2014, 16:20

... ethidium bromide. Labeling proteins with protein kinase C Fifty picomols of the indicated mutant topoisomerase I proteins were incubated with 20 ng of protein kinase C (Upstate Biotechnology, Inc., ... relaxation [4]. The cleavage religation reaction catalyzed by human DNA topoisomerase I, the prototypical type IB enzyme, is essential for many biological processes, Keywords...

Ngày tải lên: 29/03/2014, 22:21

... in different media, exhibit mild defects in protein synthesis a nd are sensitive to several protein synthesis inhibitors [49]. Multiple copies of SAL6 cause antisuppression of nonsense mutations ... characterized in the budding yeast K luy verom yces l ac tis [40]. The protein is highly similar to that of S. cerevisiae (93% identity) and it has a bro ad role i n modulating multidrug r...

Ngày tải lên: 17/03/2014, 23:20

... no history of Cesarean section but had 2 spontaneous abortions treated by dilatation and curettage. Fluid collection during pregnancy is sometimes considered insignificant if the vital signs ... emergent situation [5]. In conclusion, this report highlights the significance of a history of spontaneous abortion treated by dilatation and curettage in uterine rupture caused by place...

Ngày tải lên: 25/10/2012, 10:56

... levels. Key words: Helicobacter pylori; Reactive Oxygen Species; Mitochondrial DNA; Mutation. Introduction Helicobacter pylori (Hp) are Gram-negative mi- croaerophilic bacteria. Hp infection represents ... key factor in the etiology of various gastrointestinal dis- eases, ranging from chronic active gastritis without clinical symptoms to peptic ulceration, gastric ade- nocarcinoma, and...

Ngày tải lên: 25/10/2012, 11:18

... so we investigated the efficacy of PSL co-administration in this study. Statistical analysis The grade of each histopathological finding was analyzed by the Wilcoxon rank sum test in each ... Women’s University. Drugs Navelbine ® Injection (VNR) and Adriacin ® Injec- tion (DXR) were kindly provided by Kyowa Hakko Kirin Co. (Tokyo, Japan). A 10 mg/mL vial of VNR was diluted with...

Ngày tải lên: 26/10/2012, 09:57

... example of an inhibitory actio n by antibiotics in vivo is the inhibition of prokaryotic protein synthesis [15±18]. For instance, macrolide antibiotics, espe- cially erythromycin (Fig. 1 A), are ... by tetracycline is n egligible (Fig. 2). The sixfold stron ger splicing inhibition by Cl-tetracycline may be explained by the a dditional s ize and hydrophobicity introduced by the sub...

Ngày tải lên: 21/02/2014, 15:20

... gastric lipase, and endothelial lipase [1,2]. The primary function of LPL is triglyceride hydrolysis in triglyceride-rich lipoproteins, such as chylomicron and very low density lipoprotein (VLDL) ... Genetically engineered mice lacking LPL also exhibit hypertriglyceridemia. In addition to lipolytic activity, LPL functions as a ligand for lipoprotein receptors, such as low density lipoprotein...

Ngày tải lên: 21/02/2014, 15:20