Báo cáo Y học: Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids pot
Báo cáo Y học: Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids pot
... 2213 Heterologous expression of a Rauvol a cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids Irina Gerasimenko, Yuri Sheludko, Xueyan Ma and ... Subcellularlocalization of tryptophan decarboxylase, strictosidine synthaseand strictosidine glucosidase in suspension cultured cells of Catharanthus roseus and Tabernamontana divaricata. Plant ... reverse) and GSP5b (5¢-GTGCATACAACGAAGGCAATCGAGGTCC-3¢, reverse)using MarathonTM cDNA Amplification Kit and Advant-ageÒ cDNA polymerase from Clontech (Heidelberg,Germany) according to the manufacturer’s...
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... which are components of theeukaryotic cytoskeleton. Misfolded actin and tubulinappear to bind to the apical domain of the CCT ring[9,10] in a nearly native conformation [10,11]. Substratebinding ... a multigenic family suggests an ancient paralogy withineukaryotes. The predilection of CCT for cytoskeletalCorrespondence to Sandra Pucciarelli, Dipartimento di BiologiaM.C .A. , Universita`di Camerino, ... were then analyzed in twoseparate lanes of a nondenaturing PAGE. Unlabeled nativepig tubulin was run in a third slot to locate the position of native dimeric tubulin. The autoradiogram of the...
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Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx
... Kodak X-OMAT film for 15–60 s. Dot blotdensity was analysed byTOTALLABgel software (Phonetix,UK). The amount of SC present in each supernatant wascalculated relative to a standard preparation ... USA). The amount of IgA present in each supernatant was calculated relative to a standard preparation with known concentration.Verification of J-chain expression To examine production of J-chain, ... Design AS, Nesoddtangen, Norway;2Department of Pathology, Rikshospitalet, Norway;3Department of Biology,University of Oslo, Norway To establish stable cell lines that produce recombinantmultisubunit...
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Báo cáo khoa học: Heterologous expression of a serine carboxypeptidase-like acyltransferase and characterization of the kinetic mechanism potx
... ester-dependentacyltransferases from hydrolytic enzymes of primarymetabolism [6,8,15]. Although the analyzed SCPLacyltransferases have maintained the nature andconfiguration of the Ser-His-Asp catalytic triad fromhydrolases, ... with isobutyroyl transferase fromwild tomato and AtSCT favor Saccharomyces cerevisi-ae as heterologous host for SCPL acyltransferases [8].Similar approaches with AtSMT in our laboratory,however, ... catalyzed byenzymes of a serine carboxypeptidase-like acyltransfer-ase family? Phytochemistry 66, 1334–1345.34 Dahlbender B & Strack D (1986) Purification and prop-erties of 1-(hydroxycinnamoyl)-glucose:1-(hydroxycinna-moyl)-glucose...
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Báo cáo y học: "The epidemiology of medical emergency contacts outside hospitals in Norway - a prospective population based study"
... of vital (life threatening) danger possibleNACA 5 Acute vital (life threatening) dangerNACA 6 Acute cardiac or respiratory arrestNACA 7 DeathZakariassen et al. Scandinavian Journal of Trauma, ... problem wasclassified (table 2).The NACA score system was chosen because it iseasy to use retrospectively and the air ambulances useZakariassen et al. Scandinavian Journal of Trauma, Resuscitation ... Hedmark, and Opplandcounties, covering a total of 69 581 km2(21% of thetotal area ofNorway) and 816 000 inhabitants (18% of the total population). Data registration was performedprospectively during...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... The activity of puri®ed CBG towards variousnitrophenyl glycosides (a- D-glucopyranoside, a- D-glucopyr-anoside, a- D-galactopyranoside, a- L-arabinopyranoside,b-L-arabinopyranoside, a- L-arabino-furanoside, ... carbohydrates with retention of anomeric con®guration. They are widely present in naturewhere they demonstrate catalytic activity against a broadrange of b-D-glycosides.In humans, sev eral ... a- L-arabino-furanoside, a- D-man-nopyranoside, a- D-mannopyranoside, a- D-fucopyranoside, a- D-xylopyranoside, a- L-rhamnopyranoside) w as deter-mined u sing the same m ethod. Activities towards...
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Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
... Docosahexaenoic acid comprises more than 60% of the total polyunsaturated fatty acids [9]. As polyunsat-urated fatty acids are vulnerable to peroxidation by ROS,and peroxidized lipids and carbonyl compounds ... SDS/PAGE [28] andthen transferred onto Hybond-P (Amersham Pharmacia,Pscataway, NJ, U SA) under semi-dry conditions by means of a Transfer-blot SD Semi-dry transfer cell ( Bio-Rad,Tokyo, Japan). The ... on a 1% agarose gel containing 2.2Mformaldehyde [30]. Thesize-fractionated RNAs w ere transferred onto a Hybond-Nmembrane (Amersham P harmacia Biotech) by capillaryaction. After h ybridization...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... restriction of these inflamma-tory mediators also alleviates further damage to the bone, cartilage and blood supply to necrotic femoral head [39]. Liu et al conducted a phase I clinical trial to evaluate ... was decreased (Figure 5B). At 6 w after MSC trans-plantation, the shape of femoral head was integrity and the articular cartilage largely preserved. The ar-ticular cartilage was glossy and smooth, ... femoral head. The normal femoral head was smooth and round, and the articular cartilage was transparent and glossy (A) . After freezing, the femoral head was pale in the absence of normal glossiness...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... nrdF+gene was sequenced by a primer walkingapproach. For DNA analysis, dnastar software (DNAS-TAR Inc., Madison, WI, USA) and clone manager 5.0(Scientific & Educational Software, Cary, NC, USA) ... successfulpurification of high amounts of the native C. ammoni-agenes R2F as a manganese- and tyrosyl radical-con-taining metallocofactor, which was recently crystallizedas a manganese protein ... Clara, CA,USA). Data acquisition was performed using the Brukersoftware, as described above.Analysis of metalsManganese and iron have been determined by GF-AAS andICP-MS. [As a result of...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... sulfate fraction; lane 4, recombinant NaoA afterSephadex G75 chromatography; lane 5, purified recombinant NaoAafter DEAE-Sepharose Fast Flow chromatography; lane 6, standardmolecular mass markers ... saturmus var. mrakii [14], and the inhibitory effects of various compounds on NaoA activity are also similar to 2-nitropropane dioxygenase. Therefore, NaoA is possibly a nitroalkane dioxygenase-like ... enzymesin organisms may have the physiological significance of inactivating the natural defenses of plants [10]. The reactionmechanisms of several nitroalkane-oxidizing enzymes havebeen analyzed...
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