Báo cáo Y học: Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids pot

... 2213 Heterologous expression of a Rauvol a cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids Irina Gerasimenko, Yuri Sheludko, Xueyan Ma and ... Subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus r...

Ngày tải lên: 24/03/2014, 03:21

... which are components of the eukaryotic cytoskeleton. Misfolded actin and tubulin appear to bind to the apical domain of the CCT ring [9,10] in a nearly native conformation [10,11]. Substrate binding ... a multigenic family suggests an ancient paralogy within eukaryotes. The predilection of CCT for cytoskeletal Correspondence to Sandra Pucciarelli, Dipartimento di Biologia M.C...

Ngày tải lên: 23/03/2014, 21:20

... Kodak X-OMAT film for 15–60 s. Dot blot density was analysed by TOTALLAB gel software (Phonetix, UK). The amount of SC present in each supernatant was calculated relative to a standard preparation ... USA). The amount of IgA present in each supernatant was calculated relative to a standard preparation with known concentration. Verification of J-chain expression To examine prod...

Ngày tải lên: 18/03/2014, 01:20

... ester-dependent acyltransferases from hydrolytic enzymes of primary metabolism [6,8,15]. Although the analyzed SCPL acyltransferases have maintained the nature and configuration of the Ser-His-Asp catalytic triad from hydrolases, ... with isobutyroyl transferase from wild tomato and AtSCT favor Saccharomyces cerevisi- ae as heterologous host for SCPL acyltransferases [8]. Similar approac...

Ngày tải lên: 23/03/2014, 07:20

... of vital (life threatening) danger possible NACA 5 Acute vital (life threatening) danger NACA 6 Acute cardiac or respiratory arrest NACA 7 Death Zakariassen et al. Scandinavian Journal of Trauma, ... problem was classified (table 2). The NACA score system was chosen because it is easy to use retrospectively and the air ambulances use Zakariassen et al. Scandinavian Journal of Trauma,...

Ngày tải lên: 25/10/2012, 09:56

... The activity of puri®ed CBG towards various nitrophenyl glycosides (a- D -glucopyranoside, a- D -glucopyr- anoside, a- D -galactopyranoside, a- L -arabinopyranoside, b- L -arabinopyranoside, a- L -arabino-furanoside, ... carbohydrates with retention of anomeric con®guration. They are widely present in nature where they demonstrate catalytic activity against a broad range of b...

Ngày tải lên: 08/03/2014, 16:20

... Docosahexaenoic acid comprises more than 60% of the total polyunsaturated fatty acids [9]. As polyunsat- urated fatty acids are vulnerable to peroxidation by ROS, and peroxidized lipids and carbonyl compounds ... SDS/PAGE [28] and then transferred onto Hybond-P (Amersham Pharmacia, Pscataway, NJ, U SA) under semi-dry conditions by means of a Transfer-blot SD Semi-dry transfer cell ( B...

Ngày tải lên: 17/03/2014, 17:20

... restriction of these inflamma- tory mediators also alleviates further damage to the bone, cartilage and blood supply to necrotic femoral head [39]. Liu et al conducted a phase I clinical trial to evaluate ... was decreased (Figure 5B). At 6 w after MSC trans- plantation, the shape of femoral head was integrity and the articular cartilage largely preserved. The ar- ticular car...

Ngày tải lên: 25/10/2012, 11:18

... nrdF + gene was sequenced by a primer walking approach. For DNA analysis, dnastar software (DNAS- TAR Inc., Madison, WI, USA) and clone manager 5.0 (Scientific & Educational Software, Cary, NC, USA) ... successful purification of high amounts of the native C. ammoni- agenes R2F as a manganese- and tyrosyl radical-con- taining metallocofactor, which was recently crystallized as a m...

Ngày tải lên: 15/02/2014, 01:20

... sulfate fraction; lane 4, recombinant NaoA after Sephadex G75 chromatography; lane 5, purified recombinant NaoA after DEAE-Sepharose Fast Flow chromatography; lane 6, standard molecular mass markers ... saturmus var. mrakii [14], and the inhibitory effects of various compounds on NaoA activity are also similar to 2-nitropropane dioxygenase. Therefore, NaoA is possibly a nitroalkane dioxy...

Ngày tải lên: 31/03/2014, 08:20